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Impact of selected probiotic bacteria on juvenile host growth in gnotobiotic mouse model
Drgoňová, Veronika ; Janďourek, Ondřej (advisor) ; Konečná, Klára (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Pharmacy Author: Veronika Drgoňová Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Consultant: Mgr. Martin Schwarzer, Ph.D. Title of diploma: Impact of selected probiotic bacteria on juvenile host growth in gnotobiotic mouse model This thesis expands understanding of the ability of Lactiplantibacillus plantarum WJL bacteria to support the growth of gnotobiotic hosts in models of chronic malnutrition. It was investigated whether other bacteria also have similar capabilities and whether these traits are species- and strain-specific. Strains of Bifidobacterium longum ssp. longum and Bifidobacterium adolescentis were tested in a gnotobiotic juvenile mouse model of chronic malnutrition and their impact on the host's somatotropic axis was monitored. Concurrently, it was analysed how malnutrition affects the bacteria in the host's gut and the level of its colonization. Germ-free C57BL/6J mice were mono-colonized via intragastric gavage with the aforementioned bacterial strains. The mice were mated after confirming stable colonization and F1 male offspring were weaned on the 21st day after birth onto an experimental diet with low protein and fat content. Their growth was monitored weekly by...
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Molecular identification of selected species of lactic acid bacteria and bifidobacteria in food additives
Riegelová, Kristýna ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor)
Probiotic lactic acid bacteria and bifidobacteria are natural part of microflora of gastrointestinal tract. In the present, day they are grossly exploited in food processing industry. The aim of the work was molecular identification of bacteria of genus Lactobacillus and Bifidobacterium in complex matrices of two food additives. Total DNA was isolated from crude cell lysates by magnetic particles P(HEMA-co-GMA). Isolated DNA was amplified in genus-specific and species-specific PCRs. Amplicons were detected by agarose gel electrophoresis. Results were compared with declared specification given by producers in three different batches.
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Impact of Probiotic Bacteria on Allergic Sensitization in Type I Allergy Model
Schwarzer, Martin ; Kozáková, Hana (advisor) ; Prokešová, Ludmila (referee) ; Nevoral, Jiří (referee)
The main goal in reversing the allergy epidemic is the development of effective prophylactic strategies. Early life events, such as exposures to microbes, have a major influence on the development of balanced immune responses. Due to their ability to interact with host immune system and to modulate host immune responses probiotics, mainly bifidobacteria and lactobacilli have been used with some success in prevention of allergic disease. In order to be referred to as probiotic, bacterial strain has to undergo rigorous testing. We have selected three new Lactobacillus (L.) strains out of 24 human isolates according to their antagonistic activity against pathogenic bacteria, resistance to low pH and milieu of bile salts. Safety of these strains was proven upon intragastric administration to mice; moreover, we have shown their ability to shift cytokine Th1 - Th2 balance towards non-allergic Th1 response in isolated splenic cells. Allergen specific prophylaxis using probiotics as vehicles for mucosal delivery of recombinant allergen is an attractive concept for development of well-tolerated and effective allergy vaccines. We have shown that neonatal mono-colonization of germ-free mice with the L. plantarum NCIMB8826 strain producing the major birch pollen allergen Bet v 1 attenuates the development of...
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Molecular identification of selected species of lactic acid bacteria and bifidobacteria in food additives
Riegelová, Kristýna ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor)
Probiotic lactic acid bacteria and bifidobacteria are natural part of microflora of gastrointestinal tract. In the present, day they are grossly exploited in food processing industry. The aim of the work was molecular identification of bacteria of genus Lactobacillus and Bifidobacterium in complex matrices of two food additives. Total DNA was isolated from crude cell lysates by magnetic particles P(HEMA-co-GMA). Isolated DNA was amplified in genus-specific and species-specific PCRs. Amplicons were detected by agarose gel electrophoresis. Results were compared with declared specification given by producers in three different batches.
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