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	Studium genů determinujících degradaci fenolu a naftalenu v Rhodococcus erythropolis CCM2595 a Rhodococcus sp. NCIMB12038 Knoppová, Monika ; Kulakov, L. A. ; Larkin, M. J. ; Pátek, Miroslav The strain R. erythropolis CCM2595 is able to degrade phenol by the beta-ketoadipate pathway and the strain Rhodococcus sp. NCIMB12038 is able to degrade naphthalene via gentisate. Enzyme studies of these strains indicate that strain NCIMB12038 also contains enzymes necessary for phenol utilization and strain CCM2595 can also degrade naphthalene. Pulse field gel electrophoresis was used and southern hybridisation experiments were performed to reveal localization the genes involved in phenol and naphthalene degradation in both strains Detailed record
	Vyhledávání promotorů Corynebacterium glutamicum různě regulovaných v různých fázích růstu Phensaijai, M. ; Knoppová, Monika ; Pátek, Miroslav ; Nešvera, Jan Promoters of the amino acid-producing bacterium Corynebacterium glutamicum regulated differently in various phases of growth have been searched for. It was found that activity of promoter of the sigE gene coding for alternative sigma factor of RNA polymerase was strongly increased when the culture entered the stationary phase of growth Detailed record
	Nepřekládaná RNA indukovaná teplotním šokem u Corynebacterium glutamicum Zemanová, Martina ; Pátek, Miroslav ; Nešvera, Jan The first case of chromosomally encoded, untranslated, regulatory RNA in the amino acid-producing bacterium Corynebacterium glutamicum is described. The possible role of this novel regulatory RNA of C. glutamicum in control of cell response to external stress factors is in agreement with observed induction of various small untranslated RNAs in Escherichia coli by a variety of environmental stress conditions Detailed record
	Modulace genové exprese mutagenezí v promotorech Corynebacterium glutamicum Holátko, Jiří ; Elišáková, Veronika ; Prouza, Marek ; Phensaijai, M. ; Nešvera, Jan ; Pátek, Miroslav Studies of structure and function of promoters in C. glutamicum were used in graded expression of genes involved in biosynthetic pathway of valine (ilvBNC, ilvD, ilvE) and in competing or branching-off pathways (ilvA, leuA). Combination of particular promoter mutants resulted in tuning the whole pathway to overall higher flux of the intermediates through the pathway and in high production of valine Detailed record

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