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APPUCATION OF A SHORT-TIME HYDROPONICS FOR SELECTION OF APPROPRIATE DECIDUOUS WOODY PLANTS SPECIES FOR REMEDIATION PURPOSES
Malá, J. ; Cvrčková, H. ; Máchová, P. ; Dostál, J. ; Soudek, Petr ; Šíma, P.
A rapid trial of accumulation capacities for heavy metals Pb, Cd, and Cr of selected fast-growing deciduous woody plant species is presented. The Sorbus aucuparia, Populus tremula x P. tremuloides, Salix x blanda, S. viminalis, S. miyabeana, and S. elbursensiswere clonally micropropagated by induction of organogenesis. Samples of roots and aboveground parts from plantlets grown in aseptic conditions in agar semisolid substrate and hydroponics media supplemented by the heavy metal salts were taken at 2, 4, and 8 days of cultivation.The concentrations and distributions of the accumulated metals were determined using the ICP-OES method and verified by autoradiography. Practically, amounts of accumulated heavy metals increased linearly up to the end of the experiment and were higher in the roots than in aboveground parts in both, the agar cultures and hydroponics. It could be concluded that all tested species displayed a high efficacy of metal uptake and could represent prospective trees for phytoremediation of polluted soils.
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EVALUATION OF EFFECTIVITY OF PHYTOREMEDIATION METHODS
Soudek, Petr ; Petrová, Šárka ; Podlipná, Radka ; Fialová, Zuzana ; Malá, J. ; Benešová, Dagmar ; Kočí, V. ; Kafka, Z. ; Vaněk, Tomáš
Phytoremediation methods are very dynamically booming segment of remediation technologies in present time. These methods used plants and microorganisms for extraction, and in case of organics for degradation, of xenobiotics. Ability of plants involved in these detoxification reactions is depend not only on ability to uptake of xenobiotics and on enzymatic aparatus of tested plant species, but also on toxicity of accumulated contaminant and on toxicity of potential byproducts.
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STUDY OF ACCUMULATION AND TRANSPORT OF HEAVY METALS BY PLANT CULTURES OF POPULUS TREMULA X TREMULOIDES AND CANNABIS SATIVA IN LABORATORY CONDITIONS AND ON REAL LOCALITY
Benešová, Dagmar ; Soudek, Petr ; Petrová, Šárka ; Malá, J. ; Najman, M. ; Najmanová, P. ; Kafka, Z. ; Vaněk, Tomáš
In this research the cultures of hemp and aspen as a representative of herbs and woody species were selected. The experiments were focused on study of ability of plants to accumulated heavy metals (Pb, Cd, Zn, As a Cu) and translocated them to upper part of plants.
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