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Diagnostické biosenzory pro encefalopatie způsobené priony
Šobrová, Pavlína
Prion diseases are fatal transmisible neurodegenerative and infectious disorders (TSEs) of humans and animals, characterized by structural transition of the host-encoded cellular prion protein (PrPC) into the aberrantly folded pathologic isoform PrPSc. The main aim of this work is to summarize present information about prion diseases and their possibilities of determination pointed to electrochemical techniques. For this purpose cyclic voltammetry (CV), differential pulse voltammetry, differential pulse voltammetry Brdicka reaction and chronopotentiometric stripping analysis (CPSA) were used. The estimated detection limits were 32 ug/ml by CV, 16 ug/ml by DPV, 16 ug/ml by DPV -- Brdicka reaction and 8 ug/ml by CPSA. Subsequently, the influence of heat denaturation was observed. It clearly follows from the obtained results that signals of prion decreased linearly depending on the duration of the heat treatment at 99°C for various time intervals 0, 15, 30, 45, and 60 min. Moreover, we aimed our attention on studying of prion protein interaction with CdTe quantum dots (QDs) using electrochemistry. Primarily, we characterized electrochemical properties of QDs and the detection limit at 100 fg/ml was estimated. Further, electrochemical study of prion and QD interactions was carried out to find the most suitable conditions for sensitive detection of prion proteins. Detection limit (3 S/N) was estimated as 1 fg in 5 ul. This makes labeling of proteins with QDs of great importance due to easy applicability and possibility to use in miniaturized devices, which can be used in situ. This should open new possibilities how to determine the presence of these proteins on surgical equipment and other types of materials, which could be contagious.
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Modifikované peptidy ve spojení s kvantovými tečkami
Janů, Libor
In this study, water soluble CdTe QDs of different emission wavelengths were prepared. Microwave synthesis was used to prepare CdTe QDs coated with mercaptopropionic acid (MPA). MPA ensures biocompatibility and good water solubility of QDs. Therefore QDs can be used directly in bioanalytics. Further, QDs modified with biotinylated glutathione (GSH) were prepared. In this case mercapto group (--SH) ensures a bonding of GSH to QDs surface and biotin serves as high specific linker for streptavidin, avidin or neutravidin modified analyte. Finally, peptide mediated immuno-quantum dots were prepared. CdTe QDs were coated with heptamer HWRGWVC which is able to bind Fc region of human immunoglobulin G.
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New sensor for DNA mutation detection
Datinská, Vladimíra ; Klepárník, Karel ; Minárik, M. ; Foret, František
We present a design and synthesis of a new sensor intended for the genomic analysis in molecular cancer research. This sensor is based on the Förster resonance energy transfer (FRET) and can be used for the detection of complementary oligonucletide chains based on probe hybridization. Quantum dots (QDs) with their unique optical properties serve as suitable donors of energy in FRET.
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Electronic excitation energy transfer between quasi-zero-dimensional systems
Král, Karel ; Menšík, Miroslav
Electronic excitation energy transfer is studied theoretically within a prototype system of quantum dots using the excitonic representation of the electronic states of two quasi-zero dimensional subsystems, between which the excitation energy is transferred in a process treated as an irreversible kinetic phenomenon. The electron-phonon interaction is used to circumvent the energy conservation problem, especially when considering the uphill and downhill excitation transfer processes. The theory is studied upon utilizing a simplified model of two interacting quantum dots, both coupled to their environment. The theoretical approach is documented by numerical calculations. The results could be relevant to various cases of the electronic excitation energy transfer between quasi zero dimensional nanostructures.
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Sensor based on Förster resonance energy transfer with quantum dot
Lišková, Marcela ; Datinská, Vladimíra ; Klepárník, Karel ; Foret, František
Förster resonance energy transfers (FRET) are based on the nonradiative transfer of energy between a donor and acceptor dye in the distance 1-10 nm. With respect to this, quantum dots (QDs) with a high absorbance can serve as suitable donors of energy in FRET. Here, we present architectures of two sensors based on QD conjugates useful in imaging and diagnostics of cancer. One sensor, based on an oligonucleotide conjugate, can be used for the detection of complementary oligonucletide chains. The other was designed for the detection of enzymes inside cells.
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