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Visualization of insect morphological structures using x-ray microtomography and other modern 3D techniques
Rosová, Kateřina ; Prokop, Jakub (advisor) ; Janšta, Petr (referee)
In my bachelor thesis I describe techniques currently used for 3D visualization of morphological structures of insects. Since X-ray microtomography is currently the most attractive option for visualizing internal insect structures, more space is dedicated to its description, but the work includes more innovative techniques. Traditional techniques used in the past decades are not neglected, since they do not lose their importance in the competition of new methods. In the first part the individual methods are presented in terms of their simplified functional mechanisms and the basic steps of creation of 3D reconstructions are described. Techniques are further compared in the text in terms of their usability on different types and sizes of samples, according to the desired final resolution of the visualization, the time required to complete the observation and final visualization and in terms of invasiveness of the techniques. The last chapter summarizes the possibilities of using these innovative methods in entomology based on available studies. Keywords: Insecta, morphology, 3D visualization, SEM, CLSM, MRI, Micro-CT
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Paleofluid chemistry of orogenic gold deposits: novel analytical methods and case studies from the Bohemian Massif
Hrstka, Tomáš ; Zachariáš, Jiří (advisor) ; Koděra, Peter (referee) ; van den Kerkhof, Alfons (referee)
PALEOFLUID CHEMISTRY OF OROGENIC GOLD DEPOSITS: NOVEL ANALYTICAL METHODS AND CASE STUDIES FROM THE BOHEMIAN MASSIF Tomáš Hrstka1 1 Charles University in Prague, Faculty of Science, Institute of Geochemistry, Mineralogy and Mineral Resources, Albertov 6, Praha 2, CZ-128 43 Abstract of the Ph.D. Thesis Fluid inclusions represent a unique tool for understanding the processes leading to the formation of mineral deposits and fluid-rock interactions in general. Orogenic gold deposits in the central part of the Bohemian Massif (Libčice and Kasejovice deposits) were studied in order to provide a better understanding of their genesis. A multifaceted approach was adopted including a broad spectrum of micro-analytical methods. While traditional methods were used as the basis of this study (e.g., microthermometry, SEM and optical microscopy), the application and improvement/development of modern analytical methods (e.g., LA-ICP- MS and Raman spectroscopy) or introduction of alternative innovative techniques (CLSM, Nano- tomography, QEMSCAN) constituted a significant part of this study. This study reveals the importance of the HCO3 - species in hydrothermal fluids (i.e., >100 řC to ~350 řC). Previously, the prevalence of Cl- and other anions was reported for hydrothermal paleofluids and the majority of studies suggested...
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