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Methods for viability evaluation of Xanthomonas vesicatoria after low temperature plasma treatment
ZEMANOVÁ, Marta
The thesis deals with methods for viability evaluation of the phytopathogenic bacterium Xanthomonas euvesicatoria after low-temperature plasma treatment. Low-temperature plasma produced by Gliding Arc experimental device was used for treatment of X. euvesicatoria. The viability of the bacterial cells was assessed using a scanning electron microscope (SEM) and by measuring of the fluorescence in the Smart-DART device using PrestoBlue chemical reagent. Methodology has been optimised for the sample preparation for the treatment by low temperature plasma and used for evaluation of applied methods. Lethal effect of gliding arc plasma to this gram-negative bacteria was verified by SEM which showed. There is significant structural changes on the cell surface. Viability assessment of X. euvesicatoria using Smart-DART device is a fast, time-saving and inexpensive evaluation of cell viability. The great advantage of this device is its ability to measure the fluorescence in real time. The disadvantage of this method is lower reliability in current stage of research.
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Plasma Chemical Surface Treatment of Polymeric Materials for Biomedical Applications
Mikušková, Radka ; Kozáková, Zdenka (referee) ; Krčma, František (advisor)
The theoretical part of bacalor thesis summarizes a plasma application for surface modification of materials used for biomedical applications. The main focus is on polymeric materials. Practical part focuses on specific surface modifications. An aim is to obtain from the initially hydrophobic surface hydrophilic by plasma chemical surface treatment and to optimize the method. Hydrophilicity is achieved by treating the sample in low temperature low pressure plasma generated in air based gaseous mixtures. The surface treatment efficiency is characterizad by water leak through the material. Structural changes of material surface are confirmed by X-ray photoelectron spectroscopy.
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Nano-structured multicomponent plasma polymers for controlled immobilization of biomolecules
Melnichuk, Iurii ; Shukurov, Andrey (advisor) ; Čech, Vladimír (referee) ; Pavlík, Jaroslav (referee)
Title: Nano-structured multicomponent plasma polymers for controlled immobilization of biomolecules Author: Iurii Melnichuk Department / Institute: Department of Macromolecular Physics/Charles University Supervisor of the doctoral thesis: Doc. Ing. Andrey Shukurov, Ph.D. Abstract: The aim of this thesis is to highlight the feasibility of tailored nano- structures in functionalizing surfaces for biointerfacial interactions. Development of new techniques for the production of nanoscaled biomaterials can be of use in a variety of medical and biological applications, e.g. biosensors, microarrays, drug sensors, implants, blood-contacting devices. This thesis first examines the early stages of nano-structured thin film growth fabricated by vapor phase deposition of poly(ethylene). We discuss island growth within a framework of rate equation theory, dynamic scaling theory and capture zone distribution. In a second stage, we test dielectric barrier discharge to activate PE nano-pattern for covalent immobilization of proteins. Finally, we assess cell behavior on surfaces in dependence on morphology and the presence of cell adhesive protein tropoelastin. We employ plasma polymerization to produce ultrathin hydrocarbon layer capable of protein anchoring. The thesis findings for the first time manifest the critical...
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Monitoring of vitamin E content in the samples of cereals
Cibulcová, Pavla ; Borkovcová,, Ivana (referee) ; Benešová, Karolína (advisor)
Thesis deals with the analysis of vitamin E in cereal samples, namely barley and wheat and malt produced from them. Selected samples were treated with low-temperature plasma, fungicide and biological treatment to elimination fungus and mycotoxins. The aim of the thesis was to find out the influence of the treatment, the combination of several types of treatment on vitamin E content in grain and malt. If the content of health-positive substances is not reduced and grain can be used for food, malt, or for feed purposes. The theoretical part focuses on the properties, occurrence, biochemistry and importance of vitamin E for living organisms. It also focuses on the properties of cereals that are a significant source of vitamin E. Malt production and sample treatment are also described. The experimental part describes the field experiment, individual types of treatment, extraction of vitamin E from the samples and determination of vitamin E content by high performance liquid chromatography with fluorescence detection. The results are compared with the current literature. It was found that the treatment did not have a significant negative effect on vitamin E content.
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Methodology of using low-temperature plasma to sterilize the wound
Dejneka, Alexandr ; Kubinová, Šárka ; Moravec, J. ; Bezděk, D.
The main aim of the methodology is sterilization of skin surface and wounds in veterinary medicine based on low-temperature plasma. Low-temperature plasma has a non-specific antimicrobial effect and therefore can be safely applied to the living tissue without causing any damage. The methodology describes a solution for sterilization of tissues and wounds with bacterial, fungal or yeast contamination. The method can also be used for antimicrobial decontamination as well as for healing of chronic and non-healing wounds. Methodology presents solutions sterilization of tissues and wounds with potential bacterial, fungal or yeast contamination. The method can also be used for antimicrobial decontamination and promote healing of chronic and non-healing wounds.
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Study of the properties of cement composites with polypropylene fiber modified low-temperature plasma
Žižková, Lucie ; Herka, Petr (referee) ; Bodnárová, Lenka (advisor)
Plasma treatment of polypropylene fibers presents a new progressive method, how to increase the utility properties of these fibers. The thesis is focused on verifying the effect of surface treatment of polypropylene fibers in concrete with low-temperature plasma discharge in the normal atmosphere. The paper describes the procedure for treatment of polypropylene fibers with low-temperature plasma and evaluate the impact of this adjustment on the volume changes of cement composites. It should also be emphasized that the thesis is focused on the initial volume changes, ie volume changes in the early stages of solidification and only for your own mixture, which is not considered an external load. Subsequently, the experimental verification of the effect of the addition made commercially available fibers and fibers treated plasma volume changes to a selected physico-mechanical properties of the test compounds.
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Cement composite materials with polypropylene fibers
Preisner, Martin ; Hela, Rudolf (referee) ; Bodnárová, Lenka (advisor)
This thesis deals with summarizing the possible effects of the addition of polypropylene fibers, especially low temperature plasma treated on the properties of cement composites and concrete. They are given basic information and distribution of polypropylene fibers. It describes the finish polypropylene fiber low-temperature plasma. The experimental part focuses on the effect of dispersion of polypropylene fibers treated plasma in the cement matrix.
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Properties of cementitious composites with plasma treated polymer fibers
Počekajlo, Václav ; Hela, Rudolf (referee) ; Bodnárová, Lenka (advisor)
Modification of polymer fiber low temperature plasma is one of the ways to enhance the properties of modified fibers as follows. This work aims to evaluate the effect of plasma treated polypropylene fibers on the rheological properties of cement composites. The theoretical part describes the treatment and the effect of plasma treatment on polymer fibers. The influence of polypropylene fibers treated by low-temperature plasma on the workability of concrete. The practical part is described and evaluated experimental verification of the influence of the addition of commercially available fibers and fibers treated plasma on the rheological properties of fresh cement composite.
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Fibre reinforcement with plasma in cement composites
Žižková, Lucie ; Hela,, Rudofl (referee) ; Bodnárová, Lenka (advisor)
The usage of polymer fibres can be increased by the new progressive method of the plasma treatment. This thesis is focused on verification of the influence of the low-temperature treatment on polymer fibres´ surface used in concrete. The treatment of polypropylene fibres in low-temperature plasma is described in the theoretical part. The impact of the treatment on the volume differences of cement composites is also evaluated. The influence of the addition of commercially available fibres and plasma treated fibres on the volume differences of cement composites is also experimentally verified.
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Monitoring of phytopathogenic bacteria viability after low-temperature plasma treatment
ZEMANOVÁ, Marta
The bachelor´s thesis is focused on the monitoring of phytopathogenic bacteria´s viability after low-temperature plasma treatment. Gliding Arc device has been used for the plasma treatment of phytopathogenic bacteria. Evaluation was carried out using chemical reagent PrestoBlue which was followed by spectrophotometric evaluation and calculating CFU/ml from the regression equation derived from the calibration curve. The effect of the low-temperature plasma on the viability of plant pathogenic bacteria was prooved. Gram-negative bacteria Erwinia amylovora and Rahnella aquatilis were the most susceptible to the plasma treatment. These phytopathogenic bacteria belong to the family Enterobacteriaceae. Representatives of gram-positive bacteria showed lower sensitivity to the low-temperature plasma treatment. Suitable method has also been designed for the treatment of phytopathogenic bacteria by the low-temperature plasma at atmospheric pressure.
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