|
|
Characterization of eukaryotic translation initiation factor 3 subunits (eIF3) in A. thaliana male gametophyte
Linhart, Filip ; Hafidh, Said (advisor) ; Retzer, Katarzyna (referee)
From RNA-to-protein, translation initiation and protein synthesis is mediated by trans-acting factors that recognize mRNA features common to almost all eukaryotes. Eukaryotic translation initiation factor 3 complex (eIF3) is a highly conserved protein complex that recognizes 5'-CAP elements of the mRNA to initiate translation. eIF3 consists of nine subunits, three of them having two isoforms: eIF3A, eIF2B1, eIF3B2, eIF3C1, eIF3C2, eIF3D, eIF3E, eIF3F, eIF3G1, eIF3G2, eIF3H and eIF3K. This work deals with functional characterization, expression and subcellular localization of eIF3B1, eIF3B2 and eIF3E in Arabidopsis thaliana male gametophyte and interaction of eIF3E with the Constitutive photomorphogenesis 9 (COP9) complex as a regulatory complex of eIF3E post-translational control. Here we show that depletion of eif3b1 or eif3b2 is not gametophytic lethal and that the two protein might function redundantly, whereas, knockout of eIF3E causes male gametophyte lethality. Interestingly, eif3b1 show post-fertilization defects during embryogenesis, suggesting that its redundancy with eIF3B2 is restricted to the gametophyte. Gene expression studies revealed high expression of eIF3 subunits in actively dividing zones of leaf primordia, root meristem and root elongation zones as well as in the vegetative...
|
|
|
Pollen charakterisation in size segregated aerosol
Kolpakova, Anna ; Hovorka, Jan (advisor) ; Zíková, Naděžda (referee)
This bachelor thesis, in literature review, deals with bioaerosol issue, describes meaning of pollen, ways of pollen spreading and its influence on human health, summarize methods of pollen sampling from the air and common ways of pollen grains identification. In the experimental part, the use of the first stage of a High Volumetric Cascade Impactor (Hi-Vol) BGI-900 for bioaerosol sampling and subsequent SEM analysis was proved. Method of pollen separation from the impaction substrate, polyurethane foam, into homogeneous deposit on Nylon filter was optimized. Representative portion of the deposit was analyzed by SEM. There were taken 485 SEM pictures from 12 samples in 3 localities in the ČR. Pollen grains were identified in 295 SEM and determined into 9 genus and 4 families and grain deformities were quantified to be 24, 18, 50% for Prague, Brezno and Láz localities. Number of pollen increased with total aerosol mass in Prague locality only. Thre aleo identified insect secrction products borochomomes in the samples. Key words: pollen, pollen grain, bioaerosol, cascade impactor, SEM.
|
|
| |
|
|
Relationship between pollen diversity and floristic diversity - utilization for study of the past
Koďousková, Lucie ; Kuneš, Petr (advisor) ; Prach, Jindřich (referee)
This thesis deals with the relationship between palynological diversity and floristic diversity. Diversity is complex quantity, which consists of a number of species, the number of individuals and relative frequency of species known as evenness. It characterizes the diversity at the species level, genetic level or within communities. Its relationship with palynological diversity is problematic, especially because of lack of taxonomic precision in palynology, species-specific differences in pollen production and dispersal and sample size, since the number of taxa increases with sample size. The thesis also focuses on the studies which tried to prove positive correlation between palynological and floristic diversity, including techniques used. The second part describes methodology of collecting vegetational and palynological data and the treatment of sampes.
|
|
|
Evaluation of the attractiveness of individual plants and types of pollen for honey bees in depending on their action radius range in Mladá Boleslav area (Central Bohemia).
Kroupa, David ; Farkač, Jan (advisor) ; Vítězlav, Vítězlav (referee)
The aim of my bachelor thesis was to create a summary of pollen pasture of the European honey bee in the Mladá Boleslav area.
In theoretical part, I have put together basic information related to the European honey bee and pollen (bee colony, body structure, bee evolution, bee communication, pests and diseases, bee and plant relationship, harvesting and consumption of pollen, pollen load and bee products).
In the practical part, I have determined the pollen load's origin by analyzing pollen. This method was carried out with an illuminated binocular microscope. The plants are sorted into families. This data was then used in statistics, graphs and charts.
Altogether, It was collected 4 721 pollen loads between the years 2015 and 2016. I gathered 2 553 samples in 2015 and 2 168 samples in the following year. The biggest pollen pasture was offered by the Brassicaceae family in 2015 and by family Lamiaceae in 2016.
|
|
|
Abundance of honeybee
Brandejsová, Jana ; Titěra, Dalibor (advisor) ; Marcela, Marcela (referee)
Determining place thesis Abundance of bee colonies in the country and the carrying capacity of the land register. Establishing a Connection in Úvaly near Prague with three beekeepers. For these three beekeepers were required to determine reserves of pollen with three visits in the period of May, June and July. Each measurement was carried out always at the three strongest hives. Proven reserves of pollen was performed using photographs of each honeycomb in the hive. After calculating reserves of pollen, to assess the value of honey to the surrounding plants. The conclusion was to determine whether a load for the site Úvaly near Prague is present or not enough bees and suggest possible solutions.
|
|
|
Effect of grazing diversity on bee colony health status
ŠEBESTA, Tomáš
The Bachelor thesis aims at establishing the impact that diversity of bee forage has on the state of health of the bee colony. Honeybees are important for the life on this planet. The first part of the thesis presents a description of honeybees and of what is natural for, and indispensable to, their life. It presents plants that offer high- or low-quality pollen and nectar in our climate conditions during the year according to the phenological calendar, and also discusses changes in our landscape in the course of history. The second part focuses on substances that may help bees to fight against diseases and pests.
|
|
|
The application of pollen as bioindicator of the environmental state
Marečková, Kateřina ; Vávrová, Milada (referee) ; Čáslavský, Josef (advisor)
Pesticides and their excessive use lead to environmental pollution. Violation of the guidelines for their use disposal of empty containers could lead to contamination of water, soil and poisoning of animals and beneficial insects. Honey bee is useful creature on our planet. Good farming depends entirely on the pollination, but whole vegetal kingdom couldn’t exist it form known and used by mankind. Therefore, rules that protect these useful creatures against inadequate use of pesticides have been developed. This study focuses on the evaluation of the possibility to use bee products as bioindicators of the state of environment. Five active substances which are components of pesticides used in the treatment of agricultural field around Tasovice village were analysed in the pollen and honey. For sample preparation QuEChERS and SPE methods were used, gas chromatography with to mass spectrometric detection was employed as final analytical technique.
|
|
|
Utilization of bees products in cosmetics
Kobza, Martin ; Bednář, Michal (referee) ; Zemanová, Jana (advisor)
This bachelor thesis is focused on the problem of utilization of bees products in cosmetics. The aim of this thesis was to elaborate a literature review focused on individual bee products, their properties and their utilization in cosmetics. Honey, beeswax, propolis, pollen, royal jelly, apisin are said to be the bee products. Properties, utilization, determination of kvalitative properties and aplication into cosmetic products of individual bee products are mentioned in this thesis. Beeswax is used in production of lipsticks, creams and for basis of smears (oily creams). Royal jelly is aplicated into lotions, face masks, lipsticks, night creams, hair waters, hydration preparations etc. Honey is used for preparation of shower gels, soaps, body milks, hydration creams. Propolis is added into creams, body milks, body lotions, shampoos, lipsticks, soaps and mouth sprays. Pollen has significant content of vitamins, aminoacids and other substances in nature form for cosmetics. These substances are important for nutrition, regeneration and hydration of skin. Apisin is used in pharmacologic preparations, like healing creams and smears.
|
|
|
Městské parky a jejich vztah k rozvoji pylových alergií
Nedorost, Pavel
This bachelor thesis focuses on problematique of pollen allergy. In the first section this work offers overview about pollen allergies. Elements that conditions start of allergyand reaction of organism and imunity system. That is followed by possibilities to cure such conditions and overview of used medicaments. The next section is dealing with survey of people suffering from pollen allergies about their awareness about their conditon, furthermore it maps one particular town park. Finally in the annex is offered overview of selected common woody plants often found in parks in Europe.
|
guest ::
