"CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./" - Výsledky hledání - Digitální repozitář

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	Oriented immobilization of PNgase F on a porous polymer monolith for rapid N-glycan release Szekrényes, A. ; Křenková, Jana ; Keresztessy, Z. ; Foret, František ; Guttman, A. We demonstrate a simple and rapid method for the oriented immobilization of peptide-N4-(Nacetyl- glucosaminyl) asparagine amidase F (PNGase F) on a porous polymer monolith. The oriented immobilization is based on the affinity of glutathione-S-transferase (GST) tagged PNGase F towards glutathione modified monolith prepared in the capillary format. This approach allows the oriented and easily replaceable immobilization of PNGase F for rapid and efficient release of N-linked glycans. The reactor was tested by deglycosylation of several native glycoproteins such as ribonuclease B, fetuin or human immunoglobulin G. The proteins were effectively deglycosylated in several minutes and the released N-linked glycans were analyzed using off-line MALDI/MS or CE/LIF. Úplný záznam
	Thin metal films in resistivity-based chemical sensing Podešva, Pavel ; Foret, František Measurement of basic electric properties (current, voltage, resistance) is the most frequent technique in practically all scientific disciplines. Direct electric measurements are simple, precise and provide very large dynamic range. Since the related equipment is simple and inexpensive sensors based on electric measurements are very attractive for many applications in chemistry, environmental sciences or medicine. In this work, we present resistivity sensor for direct measurement of thiols in liquid samples. Úplný záznam
	Filtration microcartridge and capillary isoelectric focusing for the analysis low number of microorganisms in real samples Kubesová, Anna ; Horká, Marie ; Šalplachta, Jiří ; Horký, J. At present, detection and identification of microorganisms in real biological samples needs very sensitive methods for correct diagnosis and therapy in medical practice, biotechnology, agriculture, food safety and quality etc. Accordingly, it is essential to develop new cheap techniques for pathogen identification, particularly for pathogen in complex biological samples. Pre-concentration, separation, and sensitive detection of whole cells in one step are great potential and advantage for detection of pathogens in low concentration. Combination of filtration microcartridge and capillary isoelectric focusing was developed for pre-concentration and pre-separation of microorganisms from real suspensions and the possibility its application to real samples was verified. Úplný záznam
	Differences in fingerprints of biofilm-positive and biofilm-negative Candida strains exploitable for clinical practice Vykydalová, Marie ; Horká, Marie ; Šalplachta, Jiří ; Růžička, E. ; Kahle, Vladislav In this study, we have presented differences in fingerprints of Candida parapsilosis and Candida metapsilosis as well as their biofilm-positive and biofilm-negative forms. Fingerprints of yeasts were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis, capillary isoelectric focusing, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Samples for analyses were prepared by treatment of yeasts by boiling water or ethanol. Fingerprints of tested yeasts cells have differed in all cases. This knowledge can be used for obtaining rapid and simple method which distinguishes above mentioned yeasts in standard biochemical laboratories. Úplný záznam
	The efficient approaches for enzymatic digestion of plant proteins Dyčka, Filip ; Bobáľ, P. ; Bobálová, Janette In this work the fast and efficient methods applying ultrasound and infrared radiation were employed for in-gel digestion of proteins extracted from Hordeum vulgare. These techniques can be used instead of time consuming conventional protocol prior mass spectrometry analysis. The comparative study indicates that the examined protocols are able to enhance digestion efficiency and protein identification. Úplný záznam
	Proteomic evaluation of MCF-7 human breast cancer cells after treatment with retinoic acid isomers: Preliminary insights Flodrová, Dana ; Benkovská, Dagmar ; Macejová, D. ; Bialešová, L. ; Brtko, J. ; Bobálová, Janette The effects of 9-cis retinoic acid and all-trans retinoic acid in human MCF-7 breast cancer line have been investigated. The total cell proteins were extracted and separated on 1D SDS-PAGE. The proteins were subsequently in-gel digested by trypsin and identified by MALDI-TOF/TOF. Úplný záznam
	Capillary electrophoresis with contactless conductivity detection of cationic-derivatized oligosaccharides Partyka, Jan ; Foret, František The oligosaccharides were derivatized by quaternary ammonium label and separated by CE with contactless conductivity detection. Úplný záznam
	MALDI-TOF MS of Dickeya and Pectobacterium species - Characterization of plant pathogenic bacteria Šalplachta, Jiří ; Kubesová, Anna ; Horký, J. ; Horká, Marie MALDI-TOF MS was applied to reliable characterization of Dickeya and Pectobacterium species in this study. Our results confirm suitability of suggested analytical approach for efficient bacterial differentiation of species based on species specific mass fingerprints. MALDI-TOF MS of intact bacterial cells require minimum sample handling and is less time-consuming compared to conventional methods. Úplný záznam
	Seasonal variations of metals and ions in PM1 aerosol in Brno and Šlapanice Mikuška, Pavel ; Křůmal, Kamil ; Vojtěšek, Martin ; Kubátková, Nela ; Večeřa, Zbyněk Contribution compares the concentrations of metals and ions in atmospheric aerosols fraction PM1 in Brno and Šlapanice and identifies emission sources of aerosols. Úplný záznam
	Sub-, near- and supercritical water in analytical separations and instrumentation development Karásek, Pavel ; Šťavíková, Lenka ; Hohnová, Barbora ; Planeta, Josef ; Moravcová, Dana ; Horká, Marie ; Šlais, Karel ; Roth, Michal Applications and prospects of subcritical and supercritical water in analytical separations and separation technologies are discussed and illustrated with several examples. Úplný záznam

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