National Repository of Grey Literature 4 records found  Search took 0.01 seconds. 
Comparative transcriptomics of Ixodes ricinus tick life stages
VĚCHTOVÁ, Pavlína
The proposed thesis describes stage-specific transcription of Ixodes ricinus tick. Based on the transcriptome assemblies of I. ricinus tick life stages this work describes processes typical for each I. ricinus life stage and infers the significance of methylation and glycosylation for ticks.
"DNA" barcoding is of limited value for identifying adelgids (Hemiptera: Adelgidae) but supports traditional morphological taxonomy
VĚCHTOVÁ, Pavlína
The presented study deals with adelgids (Hemiptera: Adelgidae) identification based on the sequence divergence of part of mitochondrial cytochrome ?c oxidase I (COI) gene used for ?DNA barcoding?. Analysis evaluates the DNA barcoding ability to discriminate adelgids on the genera level and it supports species identification based on morphological taxonomy. However, it failed to recognize species within species complexes.
Isolation and characterization of highly repetitive fraction of codling moth, \kur{Cydia pomonella}
VĚCHTOVÁ, Pavlína
Repetitive DNA comprises substantial part of the eukaryotic genome. ?Junk DNA?, as it was originally understood at the beginning of its discovery has attracted a lot of attention lately due to many studies proving its functional perspectives. Analysis of its dynamics, characteristics and distribution has been widely studied in organisms with monocentric chromosomes. Holokinetic system, however, was left behind in these efforts and whole image of repetitive DNA distribution and dynamics in this system remains to be elucidated. In this thesis various approaches were used to isolate and characterise repetitive DNA in the genome of the codling moth, Cydia pomonella. Satellite DNA CPSAT-1 was successfully isolated, characterised with Dot blot and Southern blot and mapped with FISH in the genome of C. pomonella. 17 microsatellite probes were used to localize microsatellite arrays in the genome of C. pomonella. Method of microsatellite FISH revealed distribution of all tested microsatellites in C. pomonella complement.
Identification of species within \kur{Adelgidae} by molecular markers
VĚCHTOVÁ, Pavlína
Four molecular markers (nuclear ITS, EF1 alpha and mitochondrial AT-Rich and COI) were tested for use in species identification of Adelgidae family. Markers EF1 alpha and COI were successfuly amplified and sequenced. Aligned sequences of those two markers were used for reconstruction of dendrogram by Minimum Evolution method with Kimura 2-Parameter model. Comparison of dendrogram with assumptions based on morfology and biology of Adelgids confirmed suitability of chosen molecular markers for reliable resolution of taxonomic relationship within Adelgidae, although further testing and markers will be required to delineate sister species with higher precision.

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