Home > Search Results: Stehlíková, Kristýna
Search:
 
Search Tips :: Simple Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Validation of HPLC Method for Determination of some Vitamins in a Multivitamin Product
 Stehlíková, Kristýna ; Mokrý, Milan (advisor) ; Kastner, Petr (referee)
Charles University in Prague Faculty of pharmacy in Hradec Králové Department of pharmaceutical chemistry and drug analysis Candidate: Mgr. Kristýna Stehlíková Consultant: RNDr. Milan Mokrý, CSc. Title of Thesis: Validation of HPLC method for determination of some vitamins in a multivitamin product. A method for simultaneous determination of concentrations of vitamins B1 (thiamine), B3 (nicotinamide), B6 (pyridoxine) and vitamin C (ascorbic acid) in a multivitamin product by means of HPLC was validated. Linearity, precision (carried out as repaetibility), accuracy, specificity and robustness were used as validation parameters. Linearity was carried out with the assistance of method of dilution of standard solutions of vitamins in range 50 - 150% of expected concentrations of particular vitamins. Solution of a multivitamin was prepared six-times for the precision experiment and then relative standard deviation was calculated. The relative standard deviation stayed in the range of 0.9 -1,61%. Accuracy was verified by analysis of model samples prepared by addition of known amount of particular vitamins to placebo. Yield varied between 97-105%. During the specificity experiments it was not found any interefence among the other compounds presented in the multivitamin product. Necessity of keeping given...
Detailed record
Validation of HPLC Method for Determination of some Vitamins in a Multivitamin Product
 Stehlíková, Kristýna ; Mokrý, Milan (advisor) ; Kastner, Petr (referee)
Charles University in Prague Faculty of pharmacy in Hradec Králové Department of pharmaceutical chemistry and drug analysis Candidate: Mgr. Kristýna Stehlíková Consultant: RNDr. Milan Mokrý, CSc. Title of Thesis: Validation of HPLC method for determination of some vitamins in a multivitamin product. A method for simultaneous determination of concentrations of vitamins B1 (thiamine), B3 (nicotinamide), B6 (pyridoxine) and vitamin C (ascorbic acid) in a multivitamin product by means of HPLC was validated. Linearity, precision (carried out as repaetibility), accuracy, specificity and robustness were used as validation parameters. Linearity was carried out with the assistance of method of dilution of standard solutions of vitamins in range 50 - 150% of expected concentrations of particular vitamins. Solution of a multivitamin was prepared six-times for the precision experiment and then relative standard deviation was calculated. The relative standard deviation stayed in the range of 0.9 -1,61%. Accuracy was verified by analysis of model samples prepared by addition of known amount of particular vitamins to placebo. Yield varied between 97-105%. During the specificity experiments it was not found any interefence among the other compounds presented in the multivitamin product. Necessity of keeping given...
Detailed record

Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English