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Preparation of nanoparticles and nanofibers with antimicrobial components
Sosková, Simona ; Veselá, Mária (referee) ; Skoumalová, Petra (advisor)
The presented diploma thesis is focused on the preparation of new materials with antimicrobial effect. Liposomes and nanofibers from polyhydroxybutyrate containing clotrimazole and natural extracts with good antifungal and antioxidant effects were prepared. The theoretical part contains examples and short description of using nanoparticles and nanofibers in cosmetics and medicine and the description of plants which have positive and potential antimycotic effects. Moreover, methods for particles and fibers characterisation were shortly described. In the experimental part, natural water and lipid extracts were prepared and spectrophotometrically characterised for the content of polyphenols, flavonoids and the antioxidant activity. Liposomes and liposomes containtng PHB were prepared from selected extracts and the encapsulation effectivity, shortterm and longterm stability via determination of polyphenols were determined. Prepared particles were characterized with DLS method (size) and zeta- potential (stability). PHB nanofibers functionalised with selected lipid extracts and clotrimazole were prepared via electrospinning and forcespinning, and examined via FLIM and FTIR-ATR methods and spectrophotometry was used for antioxidant activity and release of active substances determination. Antifungal properties of prepared particles, extracts and fibers using the test system Candida glabrata were studied. Finally, cytotoxicity of selected samples was tested with MTT assay using human keratinocytes.
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|Preparation and characterization of micro- and nanoparticles with selected drugs
Sosková, Simona ; Bokrová, Jitka (referee) ; Márová, Ivana (advisor)
The presented bachelor thesis is focused on the way to prepare combined micro- and nanoparticles containing some natural extracts with antioxidant effects, ibuprofen as the anti-inflammatory and analgetic agent and clotrimazole as the agent with an antifungal effect. The theoretical part contains an introduction of the methods of liposome preparation and a review of encapsulation techniques, methods of particles characterization, and analysis of active substances content. Furthermore, the yeasts of the genus Candida and the methods of the antimicrobial activity determination are described. The mentioned components were encapsulated into liposomes, alginate and chitosan particles. The antioxidant activity of the extracts was determined spectrophotometrically. The encapsulation effectivity of active substances into particles and the long-term stability of encapsulated components were determined and compared too. The HPLC method was used for measuring of the concentration of drugs and spectrophotometric method was used to quantify the content of polyphenols. Prepared particles were characterized with DLS method (size) and zeta- potential (stability). Finally, antibacterial properties of prepared particles and extracts using the test system Candida glabrata were studied. The antimycotic effect of particles, disturbed liposomes and extracts was compared. Finally, potential utilization of prepared liposomes in a cosmetic or pharmaceutical product is proposed.
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Preparation of nanoparticles and nanofibers with antimicrobial components
Sosková, Simona ; Veselá, Mária (referee) ; Skoumalová, Petra (advisor)
The presented diploma thesis is focused on the preparation of new materials with antimicrobial effect. Liposomes and nanofibers from polyhydroxybutyrate containing clotrimazole and natural extracts with good antifungal and antioxidant effects were prepared. The theoretical part contains examples and short description of using nanoparticles and nanofibers in cosmetics and medicine and the description of plants which have positive and potential antimycotic effects. Moreover, methods for particles and fibers characterisation were shortly described. In the experimental part, natural water and lipid extracts were prepared and spectrophotometrically characterised for the content of polyphenols, flavonoids and the antioxidant activity. Liposomes and liposomes containtng PHB were prepared from selected extracts and the encapsulation effectivity, shortterm and longterm stability via determination of polyphenols were determined. Prepared particles were characterized with DLS method (size) and zeta- potential (stability). PHB nanofibers functionalised with selected lipid extracts and clotrimazole were prepared via electrospinning and forcespinning, and examined via FLIM and FTIR-ATR methods and spectrophotometry was used for antioxidant activity and release of active substances determination. Antifungal properties of prepared particles, extracts and fibers using the test system Candida glabrata were studied. Finally, cytotoxicity of selected samples was tested with MTT assay using human keratinocytes.
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|Preparation and characterization of micro- and nanoparticles with selected drugs
Sosková, Simona ; Bokrová, Jitka (referee) ; Márová, Ivana (advisor)
The presented bachelor thesis is focused on the way to prepare combined micro- and nanoparticles containing some natural extracts with antioxidant effects, ibuprofen as the anti-inflammatory and analgetic agent and clotrimazole as the agent with an antifungal effect. The theoretical part contains an introduction of the methods of liposome preparation and a review of encapsulation techniques, methods of particles characterization, and analysis of active substances content. Furthermore, the yeasts of the genus Candida and the methods of the antimicrobial activity determination are described. The mentioned components were encapsulated into liposomes, alginate and chitosan particles. The antioxidant activity of the extracts was determined spectrophotometrically. The encapsulation effectivity of active substances into particles and the long-term stability of encapsulated components were determined and compared too. The HPLC method was used for measuring of the concentration of drugs and spectrophotometric method was used to quantify the content of polyphenols. Prepared particles were characterized with DLS method (size) and zeta- potential (stability). Finally, antibacterial properties of prepared particles and extracts using the test system Candida glabrata were studied. The antimycotic effect of particles, disturbed liposomes and extracts was compared. Finally, potential utilization of prepared liposomes in a cosmetic or pharmaceutical product is proposed.
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