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Morphogenesis of multicellular bodies in bacteria Serratia marcescens
Rieger, Tomáš
Bacterial colonies and other macroscopic formations grown on solid nutrient media surface are generally taken to be haphazard aggregations of cells localized on a single place growing and multiplying squeezed and crowded hustling and competing for place and nutrients. Little attention is paid to their appearance which is regarded just a mass of single individuals molded by their immediate surrounding. Their size, structure, shape, coloration etc. are at most noticed separately - as handy identification markers resulting from he summation of single cells' properties. A closer look soon reveals that these macroscopic properties are not cumulative mass phenomena but actually features of a unified autonomous structure resulting from controlled and sophisticated morphogenesis We present here an introductory study on the morphogenesis of colonies and similar in the bacterium Serratia marcescens. We focused our attention not only to colonies - i.e. clonal structures arising from a single cell; we also studied structures originating from cell suspensions, regenerating after mechanical disruption, resulting from close contacts between bodies, interference of close area to the final phenotype and even structures resulting from suspensions originating of different strains. Our observations and simple experimental...
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Morphogenesis of the bacterial colonies and their mutually influencing
Rieger, Tomáš ; Markoš, Anton (advisor) ; Rulík, Martin (referee) ; Kuthan, Martin (referee)
This thesis follows previous works of our group (Rieger T. et al., 2008; Cepl J. et al., 2010 and Patkova I. et al., 2012), where we focused on the morphology of the bacterial colonies Serratia marcescens and its variety caused by changing of the inoculation conditions on nutrient agar. When bacterial colonies S. marcescens are grown on nutrient agar enriched with glucose isolated enough from other colonies in its living space, it can form coloured structured colonies, which we named morphotype "fountain" (F). This morpotype becomes ideal for following studies of mutual influencing of the bacterial colonies, because of its ability of pigmentation change or structure loss caused by altering surrounding inoculation conditions. We noticed in normal sowed agar plates, that bacterial colonies, which grows in the close distance with other colonies develop their pigmentation sooner, than colonies, that grows more isolated. We studied how is this influencing happening and what are the necessary conditions for it. We proved, that different species of bacterial macrocolonies (S. marcescens - morphotype (M), S. rubidea and E. coli) emits into the nutrient agar informative signal, which makes the recipient colonies S. marcescens reacts on this signal with the same manner (X structure). It looks, that this is...
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Morphogenesis of multicellular bodies in bacteria Serratia marcescens
Rieger, Tomáš
Bacterial colonies and other macroscopic formations grown on solid nutrient media surface are generally taken to be haphazard aggregations of cells localized on a single place growing and multiplying squeezed and crowded hustling and competing for place and nutrients. Little attention is paid to their appearance which is regarded just a mass of single individuals molded by their immediate surrounding. Their size, structure, shape, coloration etc. are at most noticed separately - as handy identification markers resulting from he summation of single cells' properties. A closer look soon reveals that these macroscopic properties are not cumulative mass phenomena but actually features of a unified autonomous structure resulting from controlled and sophisticated morphogenesis We present here an introductory study on the morphogenesis of colonies and similar in the bacterium Serratia marcescens. We focused our attention not only to colonies - i.e. clonal structures arising from a single cell; we also studied structures originating from cell suspensions, regenerating after mechanical disruption, resulting from close contacts between bodies, interference of close area to the final phenotype and even structures resulting from suspensions originating of different strains. Our observations and simple experimental...
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