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Green chromatography method development for red dyes determination in soft drinks
Boháčová, Tereza ; Ramos, María Carolina Fernández (advisor) ; Nováková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Boháčová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D., Dr. Maria Carolina Fernandez Ramos Title of diploma thesis: Development of green chromatography method for red dyes determination in soft drinks Green chromatography HPLC method was used and validated for the simultaneous determination of water-soluble red dyes - Carmoisine E 122, Amaranth E 123, Ponceau 4R E 124, Erythrosine E 127, Red 2G E 128, Allura Red AC E 129. The method was applied for direct determination of Amaranth E 123 and Ponceau 4R E 124 in drinks Syrup black currant (Neli, a.s.), energy drink Kamikaze (Power Life Energy), sparkling wine Bestivo & spritzzante (not shown), Syrup Raspberry light (VESETA spol. s.r.o), and beer Skalák Malina (Pivovar Malý Rohozec). Analytical column Chromolith Performance CN 100 x 4.6 mm was used and the mobile phase contained water / 1% (v/v) acetic acid buffer with addition of amonia for pH adjustment to value 7.0. Successful separation was obtained for all the compounds using an optimized gradient elution within 7 min. Method development was performed at a temperature of 45řC and flow rate 2ml/min. The diode-array detector (DAD) was used to monitor the colorants at the...
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Column switching chromatography for mycotoxines determination in Czech beer samples
Lhotská, Ivona ; Ramos, María Carolina Fernández (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Ivona Lhotská Supervisor, consultant: María Carolina Fernández Ramos, Ph.D, Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: Column switching chromatography for mycotoxines determination in Czech beer samples A new fast and sensitive method of high performance liquid chromatography for simultaneous determination of mycotoxins ochratoxin A and citrinin using column- switching system for on-line sample pretreatment was developed. 100 µl of beer was injected directly into the chromatographic system. Isolation of analytes was performed on guard column Ascentis Express RP-C18 (5 x 4.6 mm, 2.7 µm) washing out with methanol - water solution of acetic acid 0.5% (30:70, v/v), at a flow rate 2 ml/min; time of valve switch was set on 2 minutes. The separation was performed on Ascentis Express Phenyl-Hexyl column (100 x 4.6 mm, 2.7 µm) with mobile phase of composition acetonitril - water solution of acetic acid 0.5% at a flow rate 1 ml/min. A gradient was increasing up to 75:25 linearly in time 3 - 5.5 min. The temperature 50řC was set up for column separation. Wavelengths of fluorimetric detection were set at Ex 335 nm, Em 497 nm. Analysis of one sample including on-line pretreatment was...
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Green chromatography method development for red dyes determination in soft drinks
Boháčová, Tereza ; Ramos, María Carolina Fernández (advisor) ; Nováková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Boháčová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D., Dr. Maria Carolina Fernandez Ramos Title of diploma thesis: Development of green chromatography method for red dyes determination in soft drinks Green chromatography HPLC method was used and validated for the simultaneous determination of water-soluble red dyes - Carmoisine E 122, Amaranth E 123, Ponceau 4R E 124, Erythrosine E 127, Red 2G E 128, Allura Red AC E 129. The method was applied for direct determination of Amaranth E 123 and Ponceau 4R E 124 in drinks Syrup black currant (Neli, a.s.), energy drink Kamikaze (Power Life Energy), sparkling wine Bestivo & spritzzante (not shown), Syrup Raspberry light (VESETA spol. s.r.o), and beer Skalák Malina (Pivovar Malý Rohozec). Analytical column Chromolith Performance CN 100 x 4.6 mm was used and the mobile phase contained water / 1% (v/v) acetic acid buffer with addition of amonia for pH adjustment to value 7.0. Successful separation was obtained for all the compounds using an optimized gradient elution within 7 min. Method development was performed at a temperature of 45řC and flow rate 2ml/min. The diode-array detector (DAD) was used to monitor the colorants at the...
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Column switching chromatography for mycotoxines determination in Czech beer samples
Lhotská, Ivona ; Ramos, María Carolina Fernández (advisor) ; Chocholoušová Havlíková, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Ivona Lhotská Supervisor, consultant: María Carolina Fernández Ramos, Ph.D, Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: Column switching chromatography for mycotoxines determination in Czech beer samples A new fast and sensitive method of high performance liquid chromatography for simultaneous determination of mycotoxins ochratoxin A and citrinin using column- switching system for on-line sample pretreatment was developed. 100 µl of beer was injected directly into the chromatographic system. Isolation of analytes was performed on guard column Ascentis Express RP-C18 (5 x 4.6 mm, 2.7 µm) washing out with methanol - water solution of acetic acid 0.5% (30:70, v/v), at a flow rate 2 ml/min; time of valve switch was set on 2 minutes. The separation was performed on Ascentis Express Phenyl-Hexyl column (100 x 4.6 mm, 2.7 µm) with mobile phase of composition acetonitril - water solution of acetic acid 0.5% at a flow rate 1 ml/min. A gradient was increasing up to 75:25 linearly in time 3 - 5.5 min. The temperature 50řC was set up for column separation. Wavelengths of fluorimetric detection were set at Ex 335 nm, Em 497 nm. Analysis of one sample including on-line pretreatment was...
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