|
|
Analysis of the Municipality Management Čejč
Martinková, Monika ; Peš, Radek (referee) ; Kopřiva, Jan (advisor)
The bachelor´s thesis deals with the issue of management and finance territorial autonomy units and the evaluation of the municipality management in Čejč. The first part of the work is focused on teoretical resoures of the community, budgetary system and the budget of the municipality. The second part deals with basic charakteristic municipality Čejč and analytics her managament. In the third part there are distcribed ideas for the economy improvements of situation and oppotrunities, where the municipality could obtain grants for further development.
|
|
| |
|
|
Influences on the Price of Building Land
Dittrichová, Zuzana ; Martinková, Mária (referee) ; Doležalová, Monika (advisor)
The aim of the diploma thesis is to find out and evaluate the influences affecting the price of building lands in the given region. Furthermore, the results are confronted with the valuation decree. In the theoretical part are given the definitions of building lands according to different legal regulations. In the practical part, thirty parcels are valued, divided into two localities and subsequently compared with the purchase price of the individual lands.
|
|
|
Evaluation of the Financial Situation of a Company and Proposals for its Improvement
Martínková, Marie ; Pálka, Ladislav (referee) ; Bartoš, Vojtěch (advisor)
The bachelor´s thesis deals with the evaluation of the financial situation of the company C SYSTEM CZ, a.s. within the years 2008 - 2012 on the basic of selected methods of financial analysis. The thesis is devided into four parts, the first one gives basic information about the company, the theoretical concepts of the second part is then brought into practice in the following part of the thesis. The interpretation of the results of the financial analysis is presented in the last part of the thesis. The thesis aims to evaluate the present finantial situation of the company to find proposals for its improvement.
|
|
|
The gelation of hydroxyethylcellulose and hyaluronan using citric acid
Martinková, Martina ; Krouská, Jitka (referee) ; Hurčíková, Andrea (advisor)
This master's thesis deals with gelation of hydroxyethylcellulose and hyaluronan crosslinked with nontoxic crosslinking agent – citric acid. First of all, the optimalization of gelation process of hydroxyethylcellulose took place. After obtaining the product insolube in the ultrapure deionized water, a reaction mixture of hyaluronan and hydroxyethylcellulose in two different weight ratios were prepared. There was prepared solution containing only hyaluronan as the polymeric part too. Citric acid was used in concentrations of 5 %, 10 % and 20 % (w/w of polymer). These solutions of polymers and citric acid were gelated under the same conditions and the products were compared to each other using Differential Scanning Calorimetry, Thermogravimetry and Fourier Transform Infrared Spectroscopy. The products containing hydroxyethylcellulose and hyaluronan with hydroxyethylcellulose in weight ratio equal to 3 : 7 were considered crosslinked.
|
|
|
Analysis of chipped industry from Late Neolithic site at Lochenice
Martinková, Michaela ; Stolz, Daniel (advisor) ; Popelka, Miroslav (referee)
The presented diploma thesis presents the procedures and results of the analysis, carried out on an unpublished set of 3158 pieces of chipped industry, which has not yet been part of the rescue archaeological research, carried out on the area of the D11 highway near Lochenice (Hradec Králové district). The examined area had the designation D11-06-6B within the sectional division. Currently, it is the most extensive processed collection from the entire Czech basin, which belongs to the IV. phase of Stroked Pottery culture.
|
|
|
Bias correction of regional climate model outputs: statistical transformations of precipitation series from the climate model ALADIN in the project PERUN
Martínková, Marta
The outputs of regional climate models are biased. Regarding the bias correction of outputs from a climate model, the two fundamental approaches exist. First approach (e.g., Delta change method) gets the information on climate signal from comparison of the model control and future periods. Such information (change factor) is then applied to modify the observational data. Bias correction method gets the information on model bias from comparison of observational data and model outputs for the control period. The model outputs for future period are than corrected using this information on the model bias. This contribution is focused on the possibilities for bias correction of the model ALADIN (CNRM-ESM2-1) in the project PERUN and the precipitation series in daily time step for SSP5-8.5 scenario. Different statistical transformations are compared: methods based on statistical distribution, parametric transformations and non-parametric transformations (empirical quantiles method).\n
|
|
|
Phosphorylation of eukaryotic initiation factor 2α by alternative phosphate sources catalyzed by heme-regulated kinase: kinetic analysis
Ovad, Tomáš ; Martínková, Markéta (advisor) ; Heidingsfeld, Olga (referee)
master thesis Ing. Bc. Tomáš Ovad Phosphorylation of eukaryotic initiation factor 2α by alternative phosphate sour- ces catalyzed by heme-regulated kinase: kinetic analysis This master thesis focuses on the phosphorylation of the eukaryotic initiation factor 2α (eIF2α) by the heme-regulated inhibitor (HRI), a key reaction in the regulation of eukary- otic protein synthesis. Kinetic parameters of this reaction in the presence of ATP as the phosphate donor were determined previously. However, there are no reports on the potential of alternative NTPs (GTP, UTP, CTP) to serve as the sources of phosphate for this reaction. In this thesis, the wild-type HRI enzyme was produced by heterologous expression in E. coli and its kinase activity was assayed in the presence of ATP, GTP, UTP, and CTP. Kinetic parameters and heme half-maximal inhibitory concentrations for the HRI kinase reaction with the use of each NTP as a phosphate donor were determined. To validate these kinetic experiments, contaminations by ATP in the solutions of GTP, UTP, and CTP were excluded with the aid of ion-pair reversed-phase high-performance liquid chromatography. It has been shown that ATP is the most efficient phosphate donor for the HRI kinase reaction, although the remaining NTPs (GTP, UTP, CTP) may be utilized as the sources...
|
|
|
The two-component signal transduction system in bacteria utilizing heme analogues
Bečková, Alexandra ; Martínková, Markéta (advisor) ; Prošková, Veronika (referee)
6 Abstract Hemoproteins are essential components of both prokaryotic and eukaryotic organisms, where they play a role in fulfilling many significant life functions. According to their function, hemoproteins can be divided into several types. One specific type of hemoproteins, which was also the object of interest in this bachelor thesis, is the heme sensor proteins. The function of part of these sensor hemoproteins is the ability to detect diatomic gas molecules and subsequently bind them to the heme molecule in the protein. This process leads to changing the properties of the sensor hemoprotein and the signal then becomes part of the signalling pathways. An example of sensor hemoprotein is the histidine kinase, with a globin structure of the sensor domain, from Anaeromyxobacter sp. Fw 109 (AfGcHK), which was studied during the experimental part of the bachelor thesis. First, a transformation of E. coli BL-21 (DE3) cells with the pET21c(+)/AfGcHK plasmid was performed, followed by cell cultivation and expression of the AfGcHK protein in the cells. The expressed AfGcHK protein was isolated in two forms. One form represented AfGcHK protein containing heme, that is, with iron incorporated in its structure. The second form represented AfGcHK proteins containing a heme analogue, where nickel was incorporated...
|
|
|
Heme sensor proteins as potential biomarkers of cellular and oxidative stress processes induced by ionizing radiation
Vávra, Jakub ; Martínková, Markéta (advisor) ; Souček, Pavel (referee) ; Tichý, Aleš (referee)
[IN CZECH] Ionizing radiation is a potential inducer of the oxidative stress processes in cells. As a result, reactive radicals are formed in the intracellular space modifying the essential biomolecules. Ionizing radiation has either direct (radiation sickness) or indirect (malignant processes) effect on the organism. Therefore, a fast determination of the dose is required when suspected irradiation of the organism occurs. However, a method routinely applicable, fast enough and at the same time suitable for dose estimation based on biomarkers has not been developed so far. The aim of this thesis is to describe new properties of the selected heme sensor proteins and discuss their potential importance for the cellular adaptation to oxidative stress conditions. Specifically, the thesis is focused on two eukaryotic proteins, heme regulated inhibitor (HRI) and transcription factor p53. The study of functional regulation as well as the conformational changes of these proteins induced by heme is greatly emphasized. Besides, the optimization of the key experimental methods was conducted. Specifically, Phos-tag electrophoresis was applied for the kinetics study of HRI wild type and its Gly202Ser mutant form, which is a characteristics of lung cancer development. Unsurprisingly, for both HRI forms studied,...
|
guest ::
