|
|
Flow cytometry used in study of man`s infertility.
Kutová, Radka ; Jílek, Petr (advisor) ; Drahošová, Marcela (referee)
Background: Currently infertility of couples is a major problem, whereas the share of man and woman in infertility disorder is alike. It is important to discover the cause of infertility as soon as possible. The diagnostic possibilities of male infertility detection include the examination of sperm by flow cytometry and microscopic examination of semen. Aims: The aim of our work is to draw up an overview of the possible causes of male infertility and a summary of possible examination of semen using flow cytometry. Next aim is to evaluate the data measured by flow cytometry obtained from men over the years 2008, 2009 and 2010 at the Institute of Clinical Immunology and Allergology University Hospital Hradec Králové, Czech Republic, and determine interdependencies between the measured sperm parameters. Further, the work deals with the methodology optimization in examination of semen using flow cytometry. Methods: Determination of sperm parameters (sperm count and leukocyte count, sperm vitality, acrosom status and presence of intra-acrosomal protein) was performed using flow cytometer Cytomics FC 500 with CXP analytic software. The method used is based on the test SpermFlow from the company Exbio. Semen samples were obtained from 163 men. Results: Statistical analysis of our data set showed that men...
|
|
| |
|
| |
|
|
Flow cytometry used in study of man`s infertility.
Kutová, Radka ; Drahošová, Marcela (referee) ; Jílek, Petr (advisor)
Background: Currently infertility of couples is a major problem, whereas the share of man and woman in infertility disorder is alike. It is important to discover the cause of infertility as soon as possible. The diagnostic possibilities of male infertility detection include the examination of sperm by flow cytometry and microscopic examination of semen. Aims: The aim of our work is to draw up an overview of the possible causes of male infertility and a summary of possible examination of semen using flow cytometry. Next aim is to evaluate the data measured by flow cytometry obtained from men over the years 2008, 2009 and 2010 at the Institute of Clinical Immunology and Allergology University Hospital Hradec Králové, Czech Republic, and determine interdependencies between the measured sperm parameters. Further, the work deals with the methodology optimization in examination of semen using flow cytometry. Methods: Determination of sperm parameters (sperm count and leukocyte count, sperm vitality, acrosom status and presence of intra-acrosomal protein) was performed using flow cytometer Cytomics FC 500 with CXP analytic software. The method used is based on the test SpermFlow from the company Exbio. Semen samples were obtained from 163 men. Results: Statistical analysis of our data set showed that men...
|
guest ::
