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The different extraction techniques using for HPLC evaluation of some drugs
Kračmarová, Alžběta ; Mokrý, Milan (advisor) ; Pohanka, Miroslav (referee)
In this study the reversed-phased HPLC method for determination of an atypical antipsychotic drug amisulpride in a rabbit plasma was developed. The amisulpride is a benzamide derivative, which antagonizes human dopamine D2/D3 receptors. The main clinical efficacy of this drug is the therapy of both negative and positive symptoms of schizophrenia and low propensity to produce extrapyramidal sight effects. A Separon SGX C18 (size of particles 7 μm) as a stationary phase, a mixture of an acetonitrile - 0,1M amonium acetate (pH 5,6) 40:60 (v/v) as a mobile phase and an ambroxol as an internal standard were used for the analysis. Before the determination a solid-phase extraction was made. The method is linear in concentrations between 200 and 800 ng/ml (y=0,0065x-0,0695, r2 =0,9930), selective and robust. The analysis time was 11 min. A teoretical part of this study is approached to methods of an extraction, which is a very important step in an analysis of a biological material.
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The impact of an injection drug administration on an oxidative stress development in laboratory Guinea pig model
Kračmarová, Alžběta ; Mokrý, Milan (advisor) ; Holas, Ondřej (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate Msc. Alžběta Kračmarová Consultant assoc. prof. Miroslav Pohanka, RNDr, PhD. Title of Thesis The impact of an injection drug administration on an oxidative stress development in laboratory Guinea pig model Injections of drugs are considered to be standard procedures used in experimental work with laboratory animals. Laboratory rodents are sensitive to a way of handling. For this reason stress caused by the injection leads to alteration of various physiological processes. Rise of body temperature, tachycardia, elevated or declined blood glucose level or oxidative imbalance may influence results in disparate experiments. Aim of our experiment was to clarify an impact of intramuscular injection on development of an oxidative imbalance. The laboratory Guinea pigs were sacrificed one hour after the intramuscular injection of saline solution. After that, blood and selected organs were collected. Ferric reducing antioxidant power (FRAP), thiobarbituric acid reactive substances (TBARS), carbonylated proteins, superoxide dismutase activity and glutathione reductase activity as markers of oxidative state and caspase 3 activity as marker of apoptosis were measured in the tissue homogenates....
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The different extraction techniques using for HPLC evaluation of some drugs
Kračmarová, Alžběta ; Mokrý, Milan (advisor) ; Pohanka, Miroslav (referee)
In this study the reversed-phased HPLC method for determination of an atypical antipsychotic drug amisulpride in a rabbit plasma was developed. The amisulpride is a benzamide derivative, which antagonizes human dopamine D2/D3 receptors. The main clinical efficacy of this drug is the therapy of both negative and positive symptoms of schizophrenia and low propensity to produce extrapyramidal sight effects. A Separon SGX C18 (size of particles 7 μm) as a stationary phase, a mixture of an acetonitrile - 0,1M amonium acetate (pH 5,6) 40:60 (v/v) as a mobile phase and an ambroxol as an internal standard were used for the analysis. Before the determination a solid-phase extraction was made. The method is linear in concentrations between 200 and 800 ng/ml (y=0,0065x-0,0695, r2 =0,9930), selective and robust. The analysis time was 11 min. A teoretical part of this study is approached to methods of an extraction, which is a very important step in an analysis of a biological material.
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