|
|
Plasmid constuction for expression of reductase from Haemonchus contortus
Klouček, David ; Matoušková, Petra (advisor) ; Laštovičková, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: David Klouček Supervisor: doc. Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Plasmid constuction for expression of reductase from Haemonchus contortus Haemonchus contortus is the most important pathogenic nematode of small ruminants, mostly sheep and goats. It causes haemonchosis leading to massive deaths of infected animals. Pharmacotherapy by approved anthelmintics is the main solution to the disease. Currently, the growing resistance to available antihelmintics is still bigger and bigger problem, leading to economical and ecological issues. There are many causes of resistance. Among the non-target site mechanisms of resistance belongs the increased activity of enzymes from the first biotransformation phase mediating carbonyl-reduction; specifically aldo/ketoreductases and dehydrogenases/reductases. Increased activity of these enzymes leads to faster drug metabolism, formation of inactive metabolites and therapy failure. The aim of the thesis was to prepare a plasmid for the expression of selected reductase, which can be used for further study of the activity and possibly design of new targeted drugs. Method, which were used during my experimental work, involved isolation of RNA, reverse...
|
|
|
The limits of the anthropological language in the works of Eduardo Viveiros de Castro and Marilyn Strathern
Klouček, David ; Grygar, Jakub (advisor) ; Tesárek, Jan (referee)
This thesis aims to compare the work of Eduardo Viveiros de Castro and Marilyn Strathern in terms of their meta-anthropological argumentation. To this end, an interpretation of the key moments of both authors' work is undertaken, with a focus on the theory of anthropology. For Eduardo Viveiros de Castro, the text focuses mainly on his conceptual work associated with the ontological turn. The concepts of perspectivism and multinaturalism and their thought context are introduced. For Marilyn Strathern, attention is focused on her melanesianist monographs in order to present the specifics of her argument, concepts such as the dividual and relationality are briefly introduced, and the author's critique of certain social science frameworks of analysis is mentioned. The intersection of the works is seen in the motif of perspective, but the work also draws attention to the limitations associated with the search for intersection. In the comparison, the author tries to create a contrast in the authors' approach by reconstructing selected parts of their arguments, which are always viewed through the logic of the other's work. The resulting confrontation shows the limits of both authors' works in terms of their concreteness and points of contention. Thus, through the optics of Strathern, de Castro does not...
|
guest ::
