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Membrane potential measurement with voltage sensitive dyes in confocal microscopy
Heczková, Monika ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
The aim of this work is to make a literature search in the measurement of membrane voltage using the voltage-sensitive dyes and suggest a method of measuring the membrane voltage on the available cells using the voltage-sensitive dye Di-4-ANEPPS and RH237. The work contains an introduction to electrophysiology of cell, explains fluorescence and typical fluorescence characteristics. The thesis contains the description of a fluorescence microscope. The document was largely devoted to characterization and distribution of voltage-sensitive dyes. The output of a design solution is a real experiment.
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Znaky krajiny a jejich vnímání veřejností
Heczková, Monika
Bachelor thesis on topic : "Landscape features and their perception by community" Several important concepts required for understanding of the presented topics and, therefore, for the development of this thesis, are clarified in the literature overview. The aim of this work was to define and photographically document landscape features of the village of Návsí as the model area. The community perception ascept was determined based on a questionnaire created with the appropriate methodology and presented to general public. Answered questionnaires were evaluated and the results used to determine the public perception of selected landscape features, in particular, to ascertain how the public perceives them, what opion the public has regarding them, which features the community considers as characteristic of the village of Návsí and which features the community wishes to be preserved.
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Education of extremely intellectualy talented pupils at primary schools
Heczková, Monika ; Kucharská, Anna (advisor) ; Zemková, Jaroslava (referee)
In present the main educational trend in The Czech Republic is integration and inclusion of pupils and students with special educational needs and pupils and students extremely talented into common primary schools. This trend affects pedagogues of primary schools as well as pupils themselves. From the date 1.1. 2005 entered into force new school legislation in which is used the legislative term "extremely talented student" for the first time. The extremely talented students should have adapted 4 educational curriculum regarding their educational needs so that they could develop their intellectual potential and fully make use of their skills and knowledge and gain educational goal without external restrictive interventions (from side of school, etc.). In all countries of Europe even elsewhere is still one of the discussed questions if extremely talented children should mainly be educated by segregated form or should be integrated into common primary schools. Pedagogues have own point of view on this problem because these are just they who have to adapt educational policy of given country. Intended research was aimed at pedagogues of primary schools. Firstly, basic information on present circumstances of education was analysed. Another stage of research was aimed at getting opinions about education of...
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Floristická obchodní jednotka - plánování, realizace a organizace prodeje
Heczková, Monika
Literary review of this thesis is focused on the typology of floristic business units. The author also deals with the definition of terms related to business and describes the establishment, implementation and sales organization in the business unit. In the work the author focuses on sales psychology and work with the customer. The work also includes its own business plan, which the author established as a basis for the company, which will be actually realized in the future. As a tool that should determine the popularity of individual types of florist business units among the citizens of the catchment area was used survey in the form of questionnaires. The evaluation of survey and its own suggestions of business plan are summarized in the results of diploma thesis.
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Education of extremely intellectualy talented pupils at primary schools
Heczková, Monika ; Kucharská, Anna (advisor) ; Zemková, Jaroslava (referee)
In present the main educational trend in The Czech Republic is integration and inclusion of pupils and students with special educational needs and pupils and students extremely talented into common primary schools. This trend affects pedagogues of primary schools as well as pupils themselves. From the date 1.1. 2005 entered into force new school legislation in which is used the legislative term "extremely talented student" for the first time. The extremely talented students should have adapted 4 educational curriculum regarding their educational needs so that they could develop their intellectual potential and fully make use of their skills and knowledge and gain educational goal without external restrictive interventions (from side of school, etc.). In all countries of Europe even elsewhere is still one of the discussed questions if extremely talented children should mainly be educated by segregated form or should be integrated into common primary schools. Pedagogues have own point of view on this problem because these are just they who have to adapt educational policy of given country. Intended research was aimed at pedagogues of primary schools. Firstly, basic information on present circumstances of education was analysed. Another stage of research was aimed at getting opinions about education of...
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Membrane potential measurement with voltage sensitive dyes in confocal microscopy
Heczková, Monika ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
The aim of this work is to make a literature search in the measurement of membrane voltage using the voltage-sensitive dyes and suggest a method of measuring the membrane voltage on the available cells using the voltage-sensitive dye Di-4-ANEPPS and RH237. The work contains an introduction to electrophysiology of cell, explains fluorescence and typical fluorescence characteristics. The thesis contains the description of a fluorescence microscope. The document was largely devoted to characterization and distribution of voltage-sensitive dyes. The output of a design solution is a real experiment.
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|
|
Znaky krajiny a jejich vnímání veřejností
Heczková, Monika
Bachelor thesis on topic : "Landscape features and their perception by community" Several important concepts required for understanding of the presented topics and, therefore, for the development of this thesis, are clarified in the literature overview. The aim of this work was to define and photographically document landscape features of the village of Návsí as the model area. The community perception ascept was determined based on a questionnaire created with the appropriate methodology and presented to general public. Answered questionnaires were evaluated and the results used to determine the public perception of selected landscape features, in particular, to ascertain how the public perceives them, what opion the public has regarding them, which features the community considers as characteristic of the village of Návsí and which features the community wishes to be preserved.
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