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The biomechanical effects of levator ani muscle laceration injuries after vaginal delivery
Křepelka, Petr ; Šifta, Petr (advisor) ; Otáhal, Jakub (referee)
Title: The biomechanical effects of levator ani muscle laceration injuries after vaginal delivery Objectives: The aim of this thesis is to sum up current knowledge about the normal structure and function of levator ani muscle and findings about dysfunctions of a pelvic floor. By means of biomechanical instruments to analyse how the lower part of levator ani muscle closes (puborectalis muscle) during unilateral avulsion lesion. On the basis of computer simulation of biomechanical properties of the pelvic floor during avulsion lesion to describe the theoretical solutions for the therapy of the main types of dysfunctions of the pelvic floor. Methods: Biomechanical analysis was used with a help of computer model of muscular pelvic floor with a help of the ABAQUS model. This model with matched properties of muscular tissue was influenced by the standard pressure which influences the pelvic floor for a person of 80 kilos standing at rest. This model simulated unilateral avulsion lesion and biomechanical variables were observed during compensatory activation of uninjured parts of levator with the activity 100%, 50% and 10%. The map of the muscular tone and the rate of movement of the muscles of the pelvic floor were evaluated. Results: Only minimal movement is observed in the intact pelvic floor during...
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Myodynamics of the support phase during different take-off tasks in human locomotion
Hojka, Vladimír ; Jelen, Karel (advisor) ; Otáhal, Jakub (referee) ; Kampmiller, Tomáš (referee)
Title: Myodynamics of the support phase during different take-off tasks in human locomotion Objectives: Six types of take-off movement were analyzed in terms of support limb kinematics, take-off dynamics and muscle activation, in order to identify differences in motor control. Methods: 14 male athletes (22.6 ± 4.4 years; 182.4 ± 5.3 cm; 74.7 ± 6.2 kg) took part in laboratory experiment. Each athlete performed six different take-off movements (running, acceleration - first and second step, long jump take-off, high jump take-off and take-off to the hurdle). System Qualisys was used to analyze kinematics of the support limb. Dynamic of the suport phase was measured with Kistler 9281 EA force- plate. ME6000 apparatus was used to measure the muscle activation. Results were processed and statistically evaluated in Matlab (MathWorks, Inc) environment. Pair ANOVA, T-test and Friedmann test were performed to identify differences between take-off movements. regression analysis was introduced to find the relationship between parameters. Results: Significant differences in take-off dynamics are realized with not so significant differences in kinematic and electromyographic parameters. high jump and long jump take-offs acted most specifically in comparison with other types of take-offs. Two typically...
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Evaluation of surgical treatment of hip joint with diagnosis of femoroacetabular impingement syndrom.
Zahradník, Petr ; Otáhal, Jakub (advisor) ; Chládek, Petr (referee)
Title: The evaluation of surgical treatment of the hip in the diagnosis of femor- acetabular impingement syndrome. Objectives: The main objective of this work is to evaluate the effect of the surgery of the hip in diagnosis of femor-acetabular hip impingement. Methods: In our work we have cooperated with 103 patients who have been operated on hip with the diagnosis of femor-acetabular hip impingement (FAI). We used standardized questionnaires of WOMAC and NAHS, which assesses functional self- care, limitations in motion and painfulness. Patients completed questionnaires twice. For the first time before surgery and second time at least one year after the surgery. The specimens of questionnaire are attached in the Annex. Results: We found out that postoperative condition is significantly different from the preoperative state in terms of improved self-care, increased range of motion and reduction of painfulness. Keywords: Femor-acetabular impingement, hip surgery, WOMAC, NAHS
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The role of nitric oxide during in pathophysiology of neurodegenerative diseases
Sikora Marečková, Věra ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
Title: The role of nitric oxide in the pathophysiology of neurodegenerative diseases Objectives: The main objective of this thesis is to evaluate the effect of nitric oxide on the formation and development of neurodegenerative diseases. Another objective was to determinate, whether NO affects by its impact processes involved in apoptosis in the CNS. Methods: The thesis is prepared in the form of research, drawing from available relevant resources. Results: Nitric oxide is widely applied in the pathophysiology of selected neurodegenerative diseases, either directly or through other reactive nitrogen and oxygen. It also affects other factors that are involved in apoptosis in the CNS. Keywords: Nitric oxide, NMDA receptors, neurodegenerative diseases, excitotoxicity, apoptosis
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Tactile discrimination and excitability of α-motoneurons
Světlíková, Tereza ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
Title of diploma thesis: Tactile discrimination and excitability of alpha motoneurons Objectives: The aim of this thesis is to detect whether tactile discrimination tasks affect the excitability of the alpha motoneurons. Methods: Seven volunteers aged between 20 and 26 years participated in this study. The H reflex, (M wave) were recorded during three control and three experimental conditions. The control conditions preceded each experimental condition. By stimulating the tibialis nerve in the popliteal fossa the H reflex was elicited and its amplitude and latency measured at rest (control) and during tactile discrimination tasks (experimental). As tactile discrimination tasks, three separate tasks were chosen-tactile stimulation, escape reaction to tactile stimulation, and two-point discrimination. We used an EMG stimulator with a constant voltage output and monophasic squared pulses, with a 0,5 ms interval. The stimulation was switched on manually every 3-5 seconds. To detect the electrical potential of the soleus muscle, we used a surface EMG device, a GrassTelefactor, with galvanic isolation complying with EU standards. The parameters measured were the latency and amplitude of the H reflex and M wave during the tactile discrimination tasks and these were then compared to the values at rest. The...
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ROS and its role in neurophysiology
Šedivcová, Pavlína ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
Name of the thesis Reactive oxygen species and their neurophysiological role Aim of the thesis Aim of the theoretical part is to provide overview of reactive oxygen species (ROS) and show their place in organism - posstive and necessary effects for the organism on the one hand and source of serious diseases on the other hand. Futhermore, the theoretical part deals with the restoration balance between the production of free radicals and antioxidants protection. Theoretical part also deals with substance FeTTPS. Aim of the experimental part is to determinate whether the application of FeTTPS affects cerebral blood flow during trancallosal stimulation with increasing frequency. Futhermore, to determinate whether the applicatication of this substance affects the slope of the curve and threshold of evoked potencials and number and duration of afterdischargers. Research method The research took place at the premises of Onstitue of Physiology, Academy of Sciences, Czech Republic. Experiments were carried out on laboratory albine Wistar rats. General anesthesia was performed to rats, stimulating and sensing electrodes were implated in epidural area of sensorimotoor cortex and Laser Doppler flow probe was implated into the contralateral hemisphere. In the first part of experiment included 11 animals. We...
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Activation of neuroglia after status epilepticus induced by intracerebroventricular application of 4-aminopyridine in the rat.
Uttl, Libor ; Otáhal, Jakub (advisor) ; Stuchlík, Aleš (referee)
Epilepsy is a chronic disease characterized by spontaneous epileptic seizures. One percent of the world population is affected by the epilepsy. The existence of proper models is crucial for study of distinct types of epilepsy. We decided to deeply describe the model of status epilepticus (SE) induction in an adult rat by the intracerebroventricular application of 4-aminopyridine (4-AP). For description of this model we used the histology and immunohistochemistry methods, the western blot analysis focused on the microglial activation markers, and the series of behavioral tests to reveal the functional influence of SE in chronical experiment (8 months). We described the ability of the 4-AP to induce SE in this model. After SE, neuron degeneration and microglial activation appeared. The areas of degenerated neurons strongly corresponded to the regions with activated microglias. By the western blotting we demonstrated the oxidative stress by an increase of 3-nitrotyrosine already 2 hours after SE. Microglial activation detected by the immunohistochemistry correlated to the observed increase in IL-1β and CD68 production after SE. Considering these results we verified the functional influence of the SE in chronical experiment. We did not observed any significant changes of cognitive and motoric functions...
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The role of nitric oxide in CNS pathologies
Uttl, Libor ; Otáhal, Jakub (advisor) ; Novotný, Jiří (referee)
Nitric oxide (NO) is significant intercellular messenger, widely used in nervous system. It is used to modulate large number of physiological functions and participates in immunity responses; however in pathological state it can be responsible for wide variety of harmful effects. The goal of my bachelor thesis is to illustrate the topic of nitric oxide in CNS pathology in the form of literature research. First part of this thesis shows the formation of NO, physiological functions influenced by NO, describes the main signaling pathway NO-cGMP and deals with the use of produced NO. Second part of this thesis briefly shows the research methods: the use and distribution of NOS inhibitors, genetically modified mice and detection of NO and its products. The last part is dedicated to selected pathological states in CNS.
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Signal decomposition in surface electromyography
Vodhánilová, Edita ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
This thesis describe decomposition of electromyographic signal into its constituent motor unit action potential trains. A size and a shape of the action potencials can provide an important information about neuromuscular function. There are described the basics of neurophysiology and physiology of muscle in the theoretic section. Mainstay part of the thesis is description of electromyography and chapter about decomposition of electromyogprahy signal, where is introduced a method of decomposition and algorithms used for decomposition. In practical part is tested a robustness of algorithms, which are contained in program called EMGLAB. The trial is performed with three different signal and results are assessed and compared by the statistical methods ANOVA and paired t-test. Key words: action potencial, EMG signal, electromyography, decomposition, EMGLAB
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