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název v anglickém jazyce není uveden
Glogarová, Kateřina ; Syková, Eva (advisor) ; Langmeier, Miloš (referee) ; Rokyta, Richard (referee)
Magnetic resonance imaging (MRI) provides a useful noninvasive method to study the long-term migration and fate of transplanted stem cells in the central nervous system in vivo. Grafted adult as well as embryonic stem cells (ESCs) labeled with superparamagnetic nanoparticles survive in the host organism and migrate preferentially into a lesion site, where they populate the damaged nervous tissue. The migration is not affected by the route of administration; the lesion is populated with the same number of cells after intracerebral grafting as after intravenous injection. Less than 3 % of transplanted mesenchymal stem cells (MSCs) in a cortical photochemical lesion differentiated into neurons and none into astrocytes, while most ESCs (70 %) differentiated into astrocytes and only 5 % into neurons. The intravenous injection of MSCs or of the mononuclear fraction of the bone marrow, which includes hematopoietic and nonhematopoietic stem cells, progenitors and lymphocytes (BMCs), as well as the mobilization of endogenous BMCs with G-CSF (granulocyte colony stimulating factor) significantly improved the recovery of hind limb motor function and sensitivity in rats with a spinal cord compression lesion and significantly increased the spared white matter volume in the center of the lesion. The recovery was most...
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Nanotechnologies in regenerative medicine
Syková, Eva
Magnetic resonance imaging provides a noninvase method to study the fate of transplanted cells in vivo. Various biocomatible scaffolds based on non-woven nanofibres (products of ELMARCO or Technical University Liberec), have been developed for bridging tissue defects and for use as 3D stem cell careiers.
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