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Evaluation of cell adhesion molecules expression in mice aorta in animal model of non-alcoholic steatohepatitis.
Matějková, Barbora ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Consultant: prof. PharmDr. Petr Nachtigal, Ph.D. Candidate: Barbora Matějková Title of Thesis: Evaluation of Cell Adhesion Molecules Expression in Mice Aorta in Animal Model of Non-alcoholic Steatohepatitis Background: The purpose of the thesis was to observe changes in the expression of ICAM-1 and VCAM-1 in mice aorta endothelium. The study compares results of a group of mice after the administration of a NASH inducing diet and a group of mice fed by a chow diet. Method: Two groups of C57BL/6 mice were used for this experiment. At the age of ten weeks the experimental group was transferred to a NASH inducing diet which contains 40 % of calories from fat and 0.2 % from cholesterol. In addition to this diet, the experimental group was fed by fructose and glucose dissolved in drinking water with the concentration of 42 g/l (called "FFC-type diet" - high saturated fat, high saturated fructose, high saturated cholesterol). This diet lasted for 25 weeks. The control group was fed by a standard diet for rodents (called "chow-type diet"). The obtained samples were analysed by immunohistochemical methods. For ICAM-1, the indirect three-step avidin- biotin complex method was used. VCAM-1 molecules were...
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Genetic diseases of the liver
Šepsová, Marika ; Doleželová, Eva (advisor) ; Tripská, Katarína (referee)
Author: Marika Šepsová Title: Genetic diseases of the liver Form: Bachelor Thesis University: Charles University, Faculty of Pharmacy in Hradec Králové Degree: Medical Laboratory Technician Liver function may be affected by various factors including genetic diseases. The aim of this bachelor thesis is to collect and summarize current information about genetic diseases of the liver. Diseases with the highest incidence in the population are Dubin-Johnson syndrome, Rotor syndrome, Crigler-Najjar syndrome and Gilbert's syndrome. They are known as inherited hyperbilirubinemia characterized by an impairment in bilirubin metabolism. These genetic diseases are very rare with exception of Gilbert's syndrome. However, despite low prevalence and incidence it is necessary not to prolong their diagnosis. Most of them do not have any complications and do not require any treatment. The exception is Crigler-Najjar's syndrome, as untreated can have fatal consequences. Wilson's disease and hereditary hemochromatosis are inherited disorders of metal metabolism. Wilson's disease is a rare disease caused by an impairment in copper metabolism. Inherited hemochromatosis causes excessive iron deposition in the liver and other organs. Alpha-1 antitrypsin deficiency is characterized by impaired alpha-1 antitrypsin protein...
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Endoglin and livers
Jozefčeková, Nikola ; Doleželová, Eva (advisor) ; Prašnická, Alena (referee)
Author: Nikola Jozefčeková Title: Endoglin and liver Form: Diploma Thesis University: Charles University, Faculty of Pharmacy in Hradec Králové Degree: Pharmacy This diploma thesis concludes the available information about endoglin, its isoforms in the liver and its impact on the liver during various pathological conditions. In the first part of the thesis describes morphology and physiology of the liver, its structure, histology and metabolic functions. Second part contains an information about endoglin, its isoforms and role in TGF-β signaling complex, expression of endoglin and regulation of the expression. In the third part are described liver diseases during which is expression of endoglin changed. This part deals with the significance of endoglin as a diagnostic and prognostic marker. Endoglin (CD105, TGF-β receptor III) is a homodimeric transmembrane glycoprotein and a membrane co-receptor TGF-β with high expression in endothelial cells. Endoglin plays an important role in the vascular development. Endoglin is involved in the processes of angiogenesis, vascular homeostasis and TGF-β signalization. It affects activity of TGF-βRII, ALK1 and ALK5 receptors. Due to the interactions with TGF-β complex, modulation of activity of ALK receptors and Smads, endoglin controls fibrotic and anti-...
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Enterobius vermicularis
Exnerová, Andrea ; Vejsová, Marcela (advisor) ; Doleželová, Eva (referee)
Andrea Exnerová Enterobius vermicularis Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical laboratory technician Background: The aim of this thesis is to create an overview of the current knowledge about Enterobius vermicularis and to perform a questionnaire study among parents. The aim of this survey is to find out informations about the progress, diagnosis and treatment of this infection and public awareness of this disease. Methods: Questionnaire study among parents, whose children have ever been infected with Enterobius vermicularis. Results: Pinworm infection is the most common among children aged 4-7 years. Half of the children who visited a pediatrician were examined. In almost 25 % of the cases, the stool sample was collected. The children were treated mostly with prescribed medication, the parents rarely chose home-treatment. In one third of the cases, the other members of the household were infected too. In the half of the cases the whole family was treated preventively. The most common symptom is rectal itching (83 %). Complications are exceptional. 60 % of the respondents incorrectly think that it is possible to get infected with pinworms from animals. The respondents most commonly called enterobiasis a disease of dirty hands. Conclusions: The...
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The effect of dietary prebiotic supplementation on neonatal development in rats
Medveďová, Martina ; Doleželová, Eva (advisor) ; Kovařík, Miroslav (referee)
Charles University, Faculty of Pharmacy in Hradec Králové, Department of Biological and Medical Sciences University of Barcelona, Faculty of Pharmacy and Food Science, Department of Biochemistry and Physiology Student: Martina Medveďová Supervisor: PharmDr. Eva Doleželová, Ph.D. Consultant: Dr. Francisco José Pérez Cano, Ph.D. Study program: Pharmacy Title of diploma thesis: The effect of dietary prebiotic supplementation on neonatal development in rats Human breast milk is considered a gold standard in infant nutrition due to its multiple evident benefits on human health. However, breastfeeding is not always possible for various reasons; thus, it is a huge challenge to provide infant formulae which best mimic the composition and effects of human milk. Prebiotics added to infant formulae may greatly contribute to these prospective notable improvements in infant nutrition. The aim of this study was to evaluate the effect of a novel dietary prebiotic supplementation (under patent) on growth, fecal consistency, and immune maturation in suckling rats. G14 Lewis suckling rats were distributed into 2 experimental groups (n = 21/group): reference and prebiotic group. Vehicle or the prebiotic was administered by oral gavage daily from day 2 to 16 of life. Animals were weighed daily and their size and...
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Effect of soluble endoglin on bile acids transport in livers
Kvasnicová, Lucia ; Doleželová, Eva (advisor) ; Jirkovský, Eduard (referee)
Lucia Kvasnicová Effect of soluble endoglin on bile acids transport in livers Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Background: Increased plasma levels of soluble form of endoglin (sEng) were observed in patients with hypercholesterolemia, cystic fibrosis associated liver disease, or hepatocellular carcinoma combined with cirrhosis. As there are not available data focused on functional aspects of sEng in the liver, the aim of this diploma thesis was to describe the effect of sEng on the mechanism of bile formation and transport of bile acids in the liver. Methods: In our study were used six months-old transgenic male mice overexpressing human sEng on CBAxC57BL/6J background (sEng) and control mice (n = 8, in each group). Expressions of transport proteins responsible for bile acids homeostasis in the liver at mRNA and protein level were assessed by qRT-PCR and Western blot. Results: High plasma levels of sEng led to decreased plasmatic levels of bile acids. mRNA and protein expression analysis revealed, that this change occurs due to posttranscriptional induction of canalicular transporters, Bsep and Mrp2. Expression of an efflux Mrp4 protein localized at the basolateral membrane of hepatocyte was decreaced, which explains the decreased plasma...
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Dynamometry as a prognostic and prediction marker
Drobná, Zuzana ; Kovařík, Miroslav (advisor) ; Doleželová, Eva (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Zuzana Drobná Supervisor: PharmDr. Miroslav Kovařík, Ph.D. Title: Dynamometry as a prognostic and prediction marker Dynamometry is a method for measuring muscle strength. It is one of the methods of biomechanical motion analysis. There are two types of dynamometry. Isokinetic dynamometry is based on isokinetic muscle contraction. In this contraction, the muscles change their length. Isometric dynamometry is based on isometric muscle contraction. The length of the muscle does not change during the contraction. In my master thesis, 22 studies were included. Of this, 18 were engaged in a dynamometer, which measured muscle strength. Studies have been focused on prediction of short and long-term mortality, prognosis of hospitalized and severely ill patients, prediction of disease symptoms (cancer, chronic obstructive pulmonary disease, liver disease). I searched for studies according to the PubMed database. In 16 studies, it was confirmed that dynamometry can be used in the present time as a reliable diagnostic, predictive and prognostic method. Dynamometry is characterized as a simple, objective, non-invasive, easily transposable and reproducible method. Keywords: dynamometer, muscle...
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Effect of soluble endoglin to endothelial cells in vitro
Klingová, Rebeka ; Fikrová, Petra (advisor) ; Doleželová, Eva (referee)
Charles Univerzity in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Rebeka Klingová Supervisor: PharmDr. Petra Fikrová, Ph.D. Title of diploma thesis: Effect of soluble endoglin to endothelial cells in vitro Aim: We determined the effects of soluble endoglin on endothelial cells, by the means of inflammatory markers. The aim of the study was to point out the possible association of the soluble endoglin with endothelial dysfunction. Methods: For our study we have selected the human endothelial cells from umbilical vein - HUVEC. We have influenced the cells with soluble endoglin in two concentrations and at two different time intervals. The results were evaluated in the statistical program, in which we have compared the control group with influenced cells and two concentrations between each other. The expressions of inflammatory markers were analyzed on the level of mRNA, using the real-time PCR method. Results: Significant changes in the expression of markers were observed on the vascular and intracellular adhesion molecule at both concentrations compared to the control group. Increased values of transcription were available also for cyclooxygenase 2 and decreased values for cadherin 5 compared to the control group. Conclusion: Changes in...
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Diagnostics of exanthematic childhood diseases
Slámová, Zuzana ; Doleželová, Eva (advisor) ; Prašnická, Alena (referee)
Zuzana Slámová Diagnostics of exanthematic childhood diseases Bachelor thesis Charles University, Faculty of Pharmacy in Hradec Králové Health bioanalytics Background: The aim of the bachelor thesis was to determine the morbidity, incidence and prevalence of selected exanthematic childhood diseases (measles, scarlet fever, rubella, fourth disease, fifth disease and chickenpox) in the period 2013 - 2016 at the Department of Clinical Microbiology and Immunology of Regional Hospital Liberec, a. s. including a comparison with occurance of diseases in the whole Czech Republic. Methods: The methodical part was focused on the methods which are the basis of specific analyzers at the Department of Clinical Microbiology and Immunology of the Regional Hospital Liberec, a. s. These methods are ELISA, CLIA, KFR, PCR and cultivation. Analyzers used to diagnose antibodies against selected diseases are Alegria® (ORGENTEC), LIAISON® XL (DiaSorin) and Chorus Trio (DIESSE). For the direct quick detection of the disease was used quantitative PCR (qPCR) method on the CFX96 Touch ™ Real-Time PCR Detection System (BioRad). Detection of bacterial agents was performed by cultivation. Results: The results were processed over the period 2013 - 2016 from data provided by the Department of Clinical Microbiology and Immunology...
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Progression of endothelial dysfunction in heart in a mouse model of atherosclerosis.
Filipová, Petra ; Němečková, Ivana (advisor) ; Doleželová, Eva (referee)
Charles University in Prague, Faculty od Pharmacy in Hradec Králové Department of Biological and Medical Sciences Progression of endothelial dysfunction in heart in a mouse model of atherosclerosis Author of diploma thesis: Petra Filipová Supervisor: RNDr. Ivana Němečková, Ph.D. Backround: Atherosclerosis is a disease when atherosclerotic lesions are formed in vessels. It represents the major cause of the heart failure and stroke. The endothelial dysfunction, which has been recorded in a large number of animals, is considered one of the main causes of atherosclerosis. In our work we have focused on the detection of the endothelial dysfunction markers in the heart of the ApoE/LDLR double knock-out mouse model. Methods: We detected changes in the expression of eNOS, p-eNOS and HO-1 in two-months and eight-months old ApoE/LDLR double knock-out mice, which were fed standard chow diet. For the semiquantitative evaluation of the observed markers we used the Western blot analysis of the homogenated left ventricle. Results: According to our results there is no difference in the expression of eNOS, p-eNOS and HO-1 in the studied mouse model of atherosclerosis in relation to age. This might mean, that the endothelial dysfunction in the coronary vessels of these mice doesn't develop. However, for further...
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