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Production of secreted aspartic proteinases by pathogenic yeasts.
Šlajchová, Andrea ; Buchta, Vladimír (advisor) ; Konečná, Klára (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Andrea Šlajchová Supervisor: Doc. RNDr. Vladimír Buchta, CSc. Title of diploma thesis: Production of secreted aspartic proteinases by pathogenic yeasts The objective of this thesis was to identify the effect of farnesol as a quorum sensing molecule, which facilitates intracellular communication of Candida albicans, on the production of secreted aspartic proteinases (Sap). The theoretical section summarizes the morphology of Candida, the virulence factors, the illnessess cause by Candida and its treatment. A separate chapter focuses on secreted aspartic proteinases and on farnesol. The strains tested came from clinical isolates belonging to the Clinical Microbiology Institute of FN Hradec Kralove; two further strains were collected as well (10231 and 25188). The ATCC 10231 strain, which did not show farnesol production but the production of farnesol acid, was included in the test. Special media contained a source of bovine hemoglobin as the only source of nitrogen and had differing pHs. The strains were first pre-incubated to exogenous farnesol and later transfered to test media at various different concentrations of farnesol. The results were read after seven days of...
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. Changes in gene expression of B cell line Ramos RA-1 after infection with Francisella tularensis.
Matyášová, Simona ; Konečná, Klára (advisor) ; Kubelková, Klára (referee)
Francisella tularensis is a facultative intracellular bacterium causing disease tularemia of different species of mammals, including humans. It is able to infect phagocytotic as well as non-phagocytotic cells. We focused on the study of B cells, that role in the infection of F. tularensis have been, until recently, regarded as not significant. Over the last 10 years several studies showed important role of B cells in innate immunity to F.tularensis infection. The aim of this thesis was to idenify changes in the expression of genes that encode molecules of cytokines produced by B cells, after F. tularensis infection, due to stimulation with bacterial recombinant protein IglC and lipopolysaccharide. These changes were examinated on B cell line Ramos RA-1. Using reverse transcriptase polymerase chain reaction, we detected these cytokines: IFN-γ, IL-10 a TGF-β. Key words: Francisella tularensis, B cells, cytokines, reverse transcriptase polymerase chain reaction
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Change monitoring of the coagulation during physiological pregnancy.
Ptáčková, Veronika ; Hronek, Miloslav (advisor) ; Konečná, Klára (referee)
This project monitored changes of coagulation during physiological pregnancy by metering of choice coagulation parameters. Includes also the summary of coagulation changes near gravid women and complications that are may their owing to come. For change monitoring was measured value of Protrombin test (PT), Activated parcial tromboplastine test (APTT) and level of fibrinogen (FBG) and D-dimer (DDI) near healthy of gravid women in time up to 20., 30. and 40. week of pregnancy. Metering was effected on Dade Behrings analyzer in haematology laboratory CHL and TC General faculty hospital Prague. Outcomes were statistically processed. Was found, that the value of coagulation tests PT (P=0,0793) and APTT (P=0,1232) not show during pregnancy any relevant changes, happens only to their mild fall in terms of physiological divide. On the contrary, level FBG (P<0,0001) and DDI (P=0,0311) during pregnancy increased. Values of all parameters depend on a week pregnancy. Pregnancy is hypercoagulation state, which is effected above all decrease fibrinolytic activities and products of placenta. Imbalance among for- and anticoagulative action is able to make many complications, threating mother also fetus. Hypercoagulation conditions bear to also found increased levels of FBG and DDI.
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