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Analysis and characterization of antimicrobial peptides by capillary electrophoresis
Tůmová, Tereza ; Monincová, Lenka ; Čeřovský, Václav ; Kašička, Václav
Capillary electrophoresis has been employed for quantitative and qualitative analysis, separation and characterization of antimicrobial peptides – halictines. In addition to purity degree of synthetic peptides, also their effective and ionic mobilities and charges within a wide pH range were determined.
Real time in vivo monitoring of cytotoxic activity of two different antimicrobial peptides lasioglossin III and lasiocepsin
Tůmová, Tereza ; Lovecká, P. ; Čeřovský, Václav ; Slaninová, Jiřina
Real-time cell analyzer dual-plate (RTCA DP) can be used to dynamically test the cytotoxic activity of potent antimicrobial peptides. In this study, we tested the activity of two different antimicrobial peptides isolated from venom reservoirs of a wild bee against normal mammalian cell line – rat intestinal epithelial cells. The cytotoxic effect monitored by RTCA DP was expressed as IC50 and compared with the results of standard cytotoxic tests.

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