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The role of immune system factors in IVF/ET success
Růžičková, Žaneta ; Madar, Jindřich (advisor) ; Pěknicová, Jana (referee)
5 Abstract The aim of this study was to evaluate the presence of receptors (CD16, CD161, NKG2D, KIR2DL4 and CD85j) on the surface of immunocompetent cells and whether their presence is involved in the in vitro fertilization (IVF) and following embryotransfer (ET) success. We observed significant difference in the age of successful versus unsuccessful patients, which is confirmed by former studies on age-dependence of IVF success. The result from cytotoxic assay confirmed, that neither the stimulation protocol, nor the PBMC source have any effect on effector function. Afterwards, we performed a large-scale analysis of surface markers, starting with healthy donors, compared to patients. Both groups of patients have lower counts of HLA-G binding KIR2DL4 or CD85j receptors bearing cells. Next we observed the expression of these two receptors on various subpopulations of cells (CD56+dim , CD56+bright , T helpers and cytotoxic T cells) and their counts. Here we observed differences in CD56 surface expression on CD56+ bright NK cells, the levels of CD56+dim NK cells in PBMC (CD16 expression on this population), levels of CD56+dim Next step was to compare the levels of cell populations between the successful and unsuccessful patients. We observed an increased surface expression of KIR2DL4 on lymphocytary PBMC of...
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Thyroid gland disorder and female reproductive dysfuntion
Melicharová, Lucie ; Madar, Jindřich (advisor)
My thesis I called Thyroid disease and reproductive disorders women. It is a subject standing at the interface between gynecology, immunology and endocrinology. Reproductive interested me during the compulsory optional courses: Immunology female reproduction and reproductive immunology. His seminary work I was writing to topic antibodies against sperm. Now I have decided to address the topic of the thyroid gland. A reason - in the future I like to pay in gynecology and obstetrics, with which the topic is very closely related. In most developed countries, it is still a current issue declining birth rate. One reasons for the shift of family planning in higher age categories, then it is lower number of children in families, but also is a big contributor of reduced reproductive ability pairs. According to statistics of infertility in economically developed countries is increasing and at present currently affects 10-15% of couples, ie every 7 to 10 pair (Ventruba, 2001). The causes of infertility is involved in 30-35% only man and woman only 35-40%. A combination of factors of both partners is present in 25% of infertile couples, and it is necessary to note that in both men and women can occur more causes simultaneously. Only 5% of couples to seek medical help to detect apparent cause sterility (Ventruba, 2001)....
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Detekce transferinu v placentách z normálních a patologických těhotenství
Králová, Alena ; Světlíková, M. ; Madar, J. ; Ulčová-Gallová, Z. ; Bukovský, A. ; Pěknicová, Jana
The placenta is one of the important sites for iron metabolism in human. It transfers the iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to distinguish placental dysfunction. Differences of transferrin expression were foundbetween normal and abnormal placentae. Variations of its expression were detected in villous syncytiotrophoblast, which is in direct contact with maternal blood. In normal placentae, the expression of transferrin in syncytium was lower comparing to placentae with distinct pathologies. The results indicate that in the case of abnormal pregnancies, the fetus may require higher levels of transferrin in order to prevent iron depletion due to the stress from the placenta dysfunction
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Antitrophoblast cell-mediated immunity /AT-CMI/ in recurrent miscarriage and ectopic pregnacy
Madar, J. ; Kučera, E. ; Šůla, K. ; Chaloupková, A. ; Pěknicová, Jana ; Tolarová, M. ; Nouza, K. ; Kinský, R.
Specific mechanisms operate in decidua nad trophoblast to establish and maintain immunological tolerance of the embryo in mother's uterus. due to the lack of this tolerance, disturbances of pregnancy may result. In this presentation, we have tested the hypothesis that anti-trohhoblast cell-mediated immunity (AT-CMI) is elevated in habitually aborting women and that extra-uterine localisation of the embryo leads to subsequent elevation of AT-CMI.
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