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Design of the selected parts of the concrete object
Hrvolová, Markéta ; Horák, David (referee) ; Švaříčková, Ivana (advisor)
The aim of the bachelor´s thesis is the static solution of the selected parts of the reinforced concrete object with drawing documentation included. Project describes the design and assessment of the monolitic slab structure and stairway slab by the first and second limit state. As the part of the thesis is the attachment describing the process of modeling the truss structure. For calculation of the internal forces was used software Scia Engineer.
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The use of magnetic microparticles for DNA isolation
Jelínek, Zdeněk ; Horák, Daniel (referee) ; Rittich, Bohuslav (advisor)
The effectiveness of magnetic microparticles in isolation of DNA from Lactobacillus rhamnosus CCM 1825T and DNA from chicken erythrocytes were studied in diploma thesis. Magnetic HEMA based microparticles coated by carboxylic groups and hyperbranched styrene-divinylbenzene particles (IMC AS ČR, Prague, Czech Republic) were used for DNA isolation. Magnetic microparticles Dynabeads® DNA DIRECT™ Universal (Dynal, Norway) based on polystyrene and MPG® Uncoated (PureBiotech, USA) based on magnetic glass were used as a control. The dependence of amount of eluted DNA on concentration of DNA in the base solution and the dependence of amount of eluted DNA on concentration of magnetic microparticles were studied. The affinity of magnetic microparticles to RNA for various concentrations of RNA solution was studied, too. The ability of tested particles to isolate DNA from real samples was validated using milk product Actimel. The quality of isolated DNA of Lactobacillus genus was proved using genus specific PCR.
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Planar antenna arrays for RFID applications
Pochobradský, Jakub ; Horák, David (referee) ; Lukeš, Zbyněk (advisor)
This thesis describes the basic principles of radio frequency identification, basic characteristics of patch antennas, the possibility of their feeding and design of planar antenna series. Are discussed, impedance matching options. The selected antenna arrays were realised, measuring their parameters was down and measured parameters was compared with simulation results.
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Design of the Timber-Concrete Composite Ceiling Structure
Válek, Jakub ; Horák, David (referee) ; Švaříčková, Ivana (advisor)
The reconstruction of existing houses is currently a frequent issue. This work deals with the possibilities of reinforcing wooden ceilings with the focus on ceiling reinforcement using an over concrete slab. This suggested ceiling reconstruction work is desirable from the point of view of the rigidity of the object in the horizontal direction. The work includes the proposition for the composite, according to various norms and their comparison regarding the economic side of the design. The work further provides a description of the experiment, its preliminary results and its comparison with the theoretical calculation.
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OpenSource SW for data traffic inspection
Horák, Daniel ; Stančík, Peter (referee) ; Hajný, Jan (advisor)
This work deals with a usage of free and open software for filtering of network traffic and its installation in network gateway in small and middle-sized networks. Main focus is on filtering the http protocol on the level of a application layer. At the start a problems of free and open software is analyzed along with its advantages that result from its usage.There are as well given reasons for choosing the Debian GNU/Linux distribution. Further there are discussed possibilities of filtering network traffic on packets level and status filter along with the soft- ware iptables. Main part focuses on a filtering of http traffic on the application layer level using program Squid and DansGuardian. In a practical part the installation and configuration of operating system Debian GNU/Linux is presented including base services (ssh, DNS and DHCP server). Further more there are installations and configurations of softwares Squid and DansGuardian demonstrated. For DansGuardian there is a web configuration interface created.
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Structural laminated wood reinforced with composite materials
Kovács, Pavel ; Horák, David (referee) ; Žítt, Petr (advisor)
This master’s thesis describes an experimental program investigating the strengthening of beams of glued laminated timber with composite reinforcement. The work compares the behavior of beams reinforced with composite reinforcement with unreinforced beam and with solid timber beam. The work also deals with identifying and evaluating the physic-mechanical properties of materials.
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Plasmid DNAs interactions with lanthanoide compounds
Budko, Kateryna ; Horák, Daniel (referee) ; Rittich, Bohuslav (advisor)
Recently much attention is given to lanthanides and their complexes as excellent catalysts for cleavage of nucleic acids. The thesis has been focused on the cleavage of plasmid and bacterial DNA by ions Nd3+ and Y3+ and by different carriers containing the lanthanide compounds. The creation of single-stranded nicks and double-stranded ones in the plasmid DNA molecules was studied by agarose gel electrophoresis. Verification of the cleavage of bacterial DNA was made by polymerase chain reaction using primers specific for the domain Bacteria and genus and species-specific primers. The results will be used in the development of the method that will allow perfect carriers`s coverage verification with the magnetic perovskit nucleus and other carriers with the lanthanide compounds.
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Software Design of Single Purpose Machine for Visual Inspection
Horák, Daniel ; Krejsa, Jiří (referee) ; Štarha, Pavel (advisor)
This master’s thesis deals with the fundamentals of machine vision application and its practical implementation. The research part is focused on the basic possibilities of image acquisition and image processing in different dimensions. The practical part describes the design of the dimension control algorithm using a 3D camera. This algorithm is then implemented in a single-purpose machine for optical dimension control.
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Preparation and Characterization of Magnetic Carriers from Hypercrosslinked Polystyrene Microspheres and their Application in a Biosensor.
Šálek, Petr ; Šňupárek, Jaromír (referee) ; Šafařík,, Ivo (referee) ; Horák, Daniel (advisor)
With the aim to develop and characterize a functionalized highly magnetic polymer carrier of micrometer size and of a narrow particle size distribution that will be suitable for biological application, hypercrosslinked microspheres were prepared. Simultaneously, the relation between structure and properties of product was observed. Condition of dispersion polymerization were optimized to obtain starting monodisperse poly(styrene-co-divinylbenzene) [P(St-DVB)] microspheres. The P(St-DVB) microspheres of different degree of crosslinking were prepared and effect of some polymerization parameters such as type of solvent, initiator, concentration and mode of DVB addition on morphology, size and particle size distribution were investigated. The starting microspheres were hypercrosslinked to obtain microporous inner structure. Hyperosslinked particles had very large specific surface area (> 1000 m2/g) and a high content of micropores (ca. 0.6 ml/g). First, P(St-DVB) microspheres were chloromethylated using three different chloromethylation agents to regulate their porous properties. Hypercrosslinking was achieved by the addition of stannic chloride as a catalyst and by increasing a temperature. The hypercrosslinked microspheres were then functionalized with sulfo- or aminogroups. The functional groups captured precipitated iron oxide inside the porous structure of the microspheres and also served as a reactive site for intended immobilization of the protein. A solution of ferrous and ferric chloride was imbibed under vacuum into the porous structure and the iron oxide was precipitated by an aqueous ammonia solution. Finally, the magnetic functionalized hypercrosslinked micropsheres were integrated into a biosensor for qualitative detection of ovalbumin.
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Isolation of bacterial DNA from foods using magnetic carriers
Bubeníková, Lucia ; Horák, Daniel (referee) ; Španová, Alena (advisor)
The aim of the work was the selective isolation of bacterial DNA with help of magnetic carriers covered by streptavidine (PGMA-NH2-STV, MPG® Streptavidin). Conditions of functionalisation of carriers using two biotinylated probes were optimized: the amount of carrier, the amount of probe, binding of biotinyled probe to streptavidine. Purified DNA Lactobacillus was used for hybridization. DNA binding to the probe (DNA/DNA hybridization) and nospecific adsorption of DNA to the carrier were tested. Target DNA eluted from the carrier was identified using PCR with primers R16-1 and LbLMA1-rev and with primers P_eub and F_eub. The amount of probe bound to the carrier was estimated using UV spectrophotometry. It was estimated that biotinyled probe can be used for functionalisation in concentration 5 pmol/µl added to the carrier in the ration carrier : probe 1:1. It was shown that nonspecific DNA adsorption to the MPG® Streptavidin is significantly lower than to the carrier PGMA-NH2-STV.Using DNA/DNA hybridization and the MPG® Streptavidin, DNA from pure culture Lactobacillus was isolated. Procedure was applicated for DNA isolation from milk products.
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