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Analysis of the Yeast Colony Structure: Peroxisomes and Autophagy
Štěpánek, Luděk
Analysis of yeast colony structure: peroxisomes and autophagy Abstract This study is a part of the project "Molecular biology and physiology of yeast communities". Yeast colonies grown on solid complex medium communicate by periodical release of volatile ammonia (PALKOVÁ et al. 1997), which is accompanied by periodical alkalization of colonies and growth substrate. Transition of colonies to the ammonia producing period of their life is accompanied by global changes of gene expression, as was shown using RNA microarrays (PALKOVÁ et al. 2002). Changes in many metabolic genes were observed, indicating activation of peroxisomal fatty acids β-oxidation, activation of glyoxylate cycle enzymes and inhibition of mitochondrial oxidative phosphorylation. These data suggest a significant switch of cellular metabolism during early alkali phase, based on peroxisomal induction and mitochondrial decline. This switch could be facilitated by autophagy, the process which can selectively or non-selectively remove unnecessary cellular components, providing the cell with energy and removing parts interfering with the new metabolism. Saccharomyces cerevisiae strain expressing peroxisomal GFP was constructed in order to visualise peroxisomes and strain containing GFP-tagged autophagic protein Atg8p was prepared to monitor...
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Interference of selected DNA viruses with apoptotic processes
Sauerová, Pavla ; Forstová, Jitka (advisor) ; Štěpánek, Luděk (referee)
This work is focused on selected DNA viruses and some of their mechanisms used for inhibition or induction of the apoptotic processes. The selected DNA viruses are Hepatitis B virus, polyomaviruses, papillomaviruses and herpesviruses. Viruses developed different strategies for fighting the host defense mechanism during their evolution. One of the host defense mechanisms that reacts against virus infection is apoptosis. In case of viruses we can observe the phenomenon of inhibition or induction of apoptosis (which both depend on the life cycle phase of the virus). The purpose of these "fighting" strategies is to ensure successful replication, virus releasing from the cell and finally to let it spread in an organism or among them. Some "fighting" strategies are similar e.g. targeting and manipulation on p53 oncosupresor level or production of Bcl-2 homologs; other strategies are very specific. Certain viruses have mechanisms which allow them to survive in a host organism for a long time.
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Swimming and the Disabled People in the Context of History
Štěpánek, Luděk ; Miler, Tomáš (advisor) ; Parkan, František (referee)
title Swimming and the disabled in the Context of History Aim: Studying thematically relevant historical publications, objective unloading acquired facts and their interpretation of the relevant angles various fields of work. A method: Historical studies results: Submissions total and comprehensive view on swimming as physical activity individuals disabilities mainly European historical context of relationship companies with individuals with disabilities, swimming activities of individuals without disabilities and health physical activity, including current perspective. Keywords: history, swimming, disability, sports games, physical education, sport, society Powered by TCPDF (www.tcpdf.org)
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