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Effect of M1043 on the expression of inflammatory markers in a mouse model of NASH - immunohistochemical analysis
Hušková, Eliška ; Němečková, Ivana (advisor) ; Kovařík, Miroslav (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Biological and Medical Sciences Study program: Laboratory diagnostics in healthcare Author: Eliska Huskova Supervisor: RNDr. Ivana Nemeckova, Ph.D. Title of thesis: The impact of M1043 on the expression of inflammatory markers in a mouse model of NASH - immunohistochemical analysis Introduction and aim of the study: The liver is an organ with many important functions in the body, such as detoxification and regulation of lipid metabolism. Impairment of these processes can lead to NASH, a disease associated with obesity and metabolic syndrome, characterized by inflammation and accumulation of fat droplets in the liver. Studies focused on NASH are important for understanding the pathophysiology and developing new therapeutic strategies. The use of mouse models allows the investigation of pathogenesis and testing of different therapeutic approaches. This study examines the effect of the anti-endoglin antibody M1043 on the expression of inflammatory markers in a mouse model of NASH. Methodology: Mice were fed a diet inducing NASH and divided into two groups, one receiving rat IgG antibody and the other one, receiving the anti-endoglin antibody M1043. The livers were collected and processed using immunohistochemical...
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Immunohistochemical analysis of the effect of M1043 on endoglin expression in a mouse model of NASH
Kubíčková, Adéla ; Němečková, Ivana (advisor) ; Kudláčková, Zděnka (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Biological and Medical Sciences Candidate: Adéla Kubíčková Supervisor of Bachelor thesis: RNDr. Ivana Němečková, Ph.D. Title of Bachelor thesis: Immunohistochemical analysis of the effect of M1043 on endoglin expression in a mouse model of NASH The morphological and functional changes of the endothelial cells of the hepatic sinusoids and endothelial dysfunction occur during nonalcoholic steatohepatitis (NASH). It has been shown that the expression of adhesion molecules (VCAM-1, ICAM-1) and endoglin increase in NASH. Endoglin affects the activation of hepatic stellate cells, which attenuate the process of fibrotization. The aim of this bachelor thesis was to detect the expression of endoglin and the adhesion molecule VCAM-1 in a mouse model of NASH after 8 weeks of CDAA diet (choline-deficient and L-amino acid-defined diet). Mice were divided into 2 groups (n=8). The control group was injected with rat IgG antibody (CDAA+rat IgG group) for 4 weeks and the CDAA+M1043 group had anti-endoglin antibody M1043. The analysis of selected proteins expression was performed by immunohistochemical method using avidin-biotin method. The results of this thesis show that there was not any reduction in the expression of VCAM- 1 or...
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Western blot analysis of markers of endothelial dysfunction in mice aorta following high-fat diet administration
Střelcová, Ivana ; Němečková, Ivana (advisor) ; Vitverová, Barbora (referee)
7 2. ABSTRACT Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Thesis: Western blot analysis of markers of endothelial dysfunction in mice aorta following high-fat diet administration Supervisor: RNDr. Ivana Němečková, Ph.D. Author: Ivana Střelcová Background: High dietary fat intake is associated with increased risk of developing various metabolic diseases, including increased cardiovascular risk. The aim of this thesis was to investigate whether the administration of the high-fat diet containing 42 % kcal of fat affects expression of selected markers of endothelial dysfunction, the key mechanism in development of cardiovascular complications. Methods: In this thesis were evaluated changes in the expression of endothelial nitric oxide synthase (eNOS), endoglin (ENG), endothelin-1 (ET-1), intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1). An experimental group of male C57BL/6J mice aged 7 months was fed the high-fat diet containing 42% kcal of fat for 12 weeks. The control group was fed a standard laboratory diet. Western blot analysis followed by detection using a chemiluminescent reagent was used to evaluate the observed markers of endothelial dysfunction Results: The results show that there were no...
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Complex morphological, immunohistochemical, molecular genetic and clinical pathological analysis of rare types of ovarian cancers
Galko, Kamila ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Ovarian carcinomas are heterogenous group of diseases comprising various histotypes. Ovarian carcinomas as a whole are associated with the highest lethality among the female genital tumors. Currently, 5 main types of carcinomas are recognized: high grade serous carcinoma, low grade serous carcinoma, endometroid carcinoma, clear cell carcinoma and mucinous carcinoma. Other types, e.g. Brenner tumors, are very rare. Ovarian carcinomas are very diverse group with various precursors, morphology, genetic aberrations, epidemiologic and clinical, features, therapy and prognosis. The most studied and understood is high grade serous carcinoma with high incidence (up to 70 % of ovarian carcinomas). The number of studies dedicated to this tumor is still increasing. Other types of carcinomas are much less frequent, therefore related studies are rarer. Due to the rapid development of targeted therapy, it is necessary to evaluate the tumors not only morphologically but also on immunohistochemical and genetic level. Detection of neoantigens may indicate the mutation load of a tumor, it is also possible to evaluate status of mismatch repair (MMR) proteins and other features leading to chromosomal aberrations. In contrast to the high grade serous carcinoma, as for other types of carcinomas, knowledge about their...
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Functional analysis of endothelial dysfunction in mice aorta following high-fat diet administration
Martínková, Adéla ; Němečková, Ivana (advisor) ; Vitverová, Barbora (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Supervisor: RNDr. Ivana Němečková, Ph.D. Author: Adéla Martínková Title of Thesis: Functional analysis of endothelial dysfunction in mice aorta following high-fat diet administration Background and aim: Endothelial dysfunction is associated with the impairment of vasodilatory response of vessel wall mediated by nitric oxide which is produced by endothelial cells under physiological conditions. Endothelial dysfunction can be induced by high fat diet (HFD) feeding in experimental animals. The aim of this diploma thesis was to determine the effect of HFD on the functional parameters of aorta, especially the effect of the vasodilatory factor acetylcholine on endothelial cells of the thoracic part of the mouse aorta. Methods: The 7 months old C57BL/6J male mice were fed high fat diet containing 42 % kcal fat for 12 weeks to induce endothelial dysfunction in the aorta. The control group was fed a standard laboratory diet during the whole experiment. Functional parameters of aorta were assessed with wire myograph 620M. Results: At the end of the experiment, the weight of the animals was statistically significantly lower in the HFD group compared to the control group of mice fed the standard...
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