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Nanoparticles formed by complexes of copolymers with low-molar-mass compounds
Vojtová, Jana
This thesis is focused on mixed systems of various copolymers (double hydrophilic block and gradient polyelectrolytes, hydrophobic graft copolymers) and low-molar-mass compounds (sodium dodecyl sulfate as a representative of a ionic surfactant or superparamagnetic iron oxides in the form of nanocrystals). The electrostatic and hydrophobic interactions in the studied systems in aqueous solutions leading to aggregation behavior and to the formation of co-assembled nanoparticles were investigated by combination of scattering and microscopy techniques, including light, X-ray and neutron scattering, electron microscopy and atomic force microscopy. Powered by TCPDF (www.tcpdf.org)
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Impact of inclusive environment for pupils attitudes towards persons with disability
Zilcher, Ladislav
This dissertation is conceived as a theoretical - empirical study, which is focused on modifications in attitudes of fourth and fifth grade pupils towards people with disabilities. The research is based on three possible influences for attitudes, (1) the effect of classmate with disability on pupils' attitudes towards people with disability, (2) teacher's approach to the issue, their inclusive teaching competences and the effect on pupils' attitudes and (3) if school and its inclusive setting (which is represented by schools with "Fair school" certificate) has any influence on pupils' attitudes towards persons with disabilities. We try to explain one elemental empirical question by dealing with these three particular research issues. Does inclusive environment have positive influence on fourth and fifth grade pupils towards people with disabilities? We decided to choose a quasiexperimental design of this study to answer this question. Two validated empirical instruments were used in the research, they were administrated on a sample that consists of 1948 pupils from fourth and fifth grades and (their) 102 teachers. The acquired data were processed by appropriate statistical methods. Our outcomes partly confirmed the assumption that inclusive environment may have an impact on pupils' attitude towards...
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Crime relating to the abuse of addictive substances
Pacovský, Josef ; Bohuslav, Lukáš (referee)
Crime relating to the abuse of addictive substances The abuse of addictive substances is an issue of the whole society, which in current globalized world, considering continuous development of drug scene, still remains topical. The abuse of addictive substances, as a significant criminogenic need, relates to all kinds of different criminal offences, both those committed by addicts and those committed against them. This includes for example property crimes, crimes against life, person and health, or endangerment. Because of such wide scope of crimes related to the abuse of addictive substances, the focus of my work is on substantive regulation of so-called drug offences, which is stated in the articles 283 - 287 of the Criminal Code. The purpose of this thesis is to analyse constituent elements of the particular drug offences, coherent case-law, as well as some questionable issues related with this kind of criminal offences. It is for instance, the issue of determining the term "greater than the small amount" and regulation of making cannabis accessible for medical use. Higher consideration is given to the questions of so called growshops and the issues of the distribution of drug addiction. The thesis is divided into general part and special part. In the general part, there is a definition of some...
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Dreams and reality in texts of authors of the Prague Linguistic Circle and the Czech Surrealist Group
Turinská, Ivona ; Vojvodík, Josef (advisor) ; Wiendl, Jan (referee)
The rigorous thesis deals with the relationship between members of The Prague Linguistic Circle and the Czech Surrealist Group in the time period between the world wars with the emphasis on the later thirties. The thesis analyzes especially the relation of literary-scientific discourse of the Czech structuralism represented by the theoretical studies of Jan Mukařovský and Roman Jakobson to artistic repertoire of texts, art works, norms and political-cultural views of the Czech surrealism. The introductory part describes the mutual co-operation and personal relationships among members of both groups; the second part of the dissertation shows the presented problems in the historical context of the crisis of the thirties. The framework of my dissertation is composed of the following paradigmatic topics: reality, objectivity, torso, dream and the subconsciousness. The last part of the paper compares surrealism and the atmosphere of the thirties with the mannerism and the period after The Thirty Years' War. The analysis is focused on reciprocal reception of the surrealistic and the structuralistic esthetical conceptions in the thirties.
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New flame retardants in the environment
Ezechiáš, Martin
In the recent years, many research articles focused on endocrine disrupting compounds in the environment. Some of these compounds are listed in a group named brominated flame retardants. However, only few articles investigated endocrine activity of several "new" brominated flame retardants. These chemicals such as 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE) or bis(2-ethylhexyl) tetrabromophthalate (TBPH) are newly used due to ban of some previously most produced brominated flame retardant mixes. In this study, we used two recombinant yeast screens to measure estrogenic, androgenic, antiestrognic and antiandrogenic activities of some alternative brominated flame retardants. We also used ligninolytic fungi to investigate biodegradation of these compounds. Our results suggest, that 2,4,6-tribromophenol (TBP) may be a new environmental endocrine disruptor. This substance showed antiestrogenic and antiandrogenic activity in our tests. 1,2-bis(2,4,6- tribromophenoxy)ethane (BTBPE) had certain antagonistic activity too. In the biodegradation experiment, only three compounds showed significant degradation during the test period. No biodegradation have been observed for other compounds. In this study, we applied gas chromatography with mass spectrometry to analyze these "new" flame retardants. Method for gas...
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Possibilities of flow cytometry in analysis of cellular response to genotoxic stress
Havelek, Radim ; Řezáčová, Martina (advisor) ; Bílková, Zuzana (referee) ; Štětina, Rudolf (referee)
Possibilities of flow cytometry in analysis of cellular response to genotoxic stress Flow cytometry is a modern tool for interrogating the immunophenotype and functional characteristics of cells. It is a technique of quantitative single cell analysis that works by sensing optical properties of cells in a flow stream with laser beam. Analysis and differentiation of the cells is based on size, granularity, and whether the cell is carrying fluorescent molecules in the form of either conjugated antibodies or dyes. The cells may be stained with fluorescent dyes, incubated with fluorogenic substrate or labelled with fluorochrome-linked antibodies specific for molecules either on the surface or in the intracellular components of the cell. Flow cytometers are multiparameter, recording several measurements on each cell. Therefore, it is possible to identify a homogeneous subpopulation within a heterogeneous population of cells. When cells are exposed to genotoxic agent, they respond quantitatively and qualitatively according to the absorbed dose and the cell type. To cope with the resulting damage to cellular DNA, the temporary cell-cycle checkpoints and DNA damage response mechanisms are activated to allow more time for effective repair. However, if these mechanisms fail or the damage is irreparable, then...
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Investigation of defects in thin metallic films
Hruška, Petr ; Čížek, Jakub (advisor)
In the present work Mg films prepared by RF magnetron sputtering were studied. Variable energy positron annihilation spectroscopy (VEPAS) was employed for investigation of defects in the Mg films. VEPAS characterization was combined with scanning electron microscopy and X-ray diffraction in order to determine grain size, phase composition and texture. The effect of different deposition rate and deposition temperature, annealing, various substrates and film thickness on the structure and amount of defects present in the Mg films was examined. Defect studies by VEPAS showed that positrons in studied Mg films are trapped at misfit dislocations and at vacancy-like defects in grain boundaries and their density can be reduced by the deposition at elevated temperature. 1
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Genome reprogramming during the first cell cycle of embryonic development
Barnetová, Irena
Genome reprogramming during the first cell cycle of embryonic development I. Barnetova, dissertation abstract The sperm head contains highly compacted genome. This compaction is mediated by protamines. Sperm protamines are replaced by cytoplasmic histones after the sperm entry into the oocyte. Beside the proteins replacement, also some epigenetic remodeling occurs. One of the most studied epigenetic remodeling in early zygotes is DNA demethylation. This phenomenon was observed in some mammals (mouse, rat, monkey) but not in some other mammals (sheep) and what is more, in some of them quite inconsistent data were published (pig, human, goat, rabbit). In our work we were mostly concentrated on porcine zygotes and attempted to explain the reasons of inconsistency in observed data. Three factors were evaluated in our work - the technique of embryo production, sperm factors, and the oocyte quality. In the first part of the study (the technique of embryo production) we compared the zygotes produced by conventional in vitro fertilization and zygotes produced by intracytoplasmic sperm injection. The epigenetic remodeling was evaluated by immunolabeling. There were no differences between zygotes produced by the two mentioned techniques. The paternal genome was not demethylated in any of zygotes. The labeling with...
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Reductive Evolution of Mitochondria - Related Organelles in Anaerobic Protist
Rada, Petr
Charles University in Prague, Faculty of Science Department of Parasitology Ph.D. study program: Parasitology Abstract of the Ph.D. Thesis Reductive Evolution of Mitochondria - Related Organelles in Anaerobic Protist Petr Rada Supervisor: Prof. RNDr. Jan Tachezy,Ph.D. Advisor: Doc. RNDr. Ivan Hrdý, Ph.D. Prague, 2011 ABSTRACT Trichomonas vaginalis and Giardia intestinalis are parasitic protists of the Excavata group. Both contain anaerobic forms of mitochondria called hydrogenosomes (Trichomonas) and mitosomes (Giardia). Hydrogenosomes produce hydrogen and ATP by substarte level phosphorylation and mitosomes represent the highly-reduced form of mitochondria that do not participate in cellular energy metabolism and ATP generation. Both types of organelles lost the majority of mitochondrial pathways and their genomes during the mitochondrion to hydrogenosome transition. Consequently, hydrogenosomes and mitosomes facilitate translocation of nuclearly encoded proteins into the matrix of the organelle as well as exchange of metabolites and ions across their membranes. Little is known about the membrane machineries required for the biogenesis of the organelle and metabolite exchange and the limited knowledge of mitosomal proteomes has been mostly gained from genomic analysis and localization studies of a few...
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