Papers

Papers 26,973 records found  beginprevious26954 - 26963next  jump to record: Search took 0.01 seconds. 
Vztah mezi modulární strukturou serin/threonin proteinkinasy eukaryontního typu StkP z Streptococcus pneumoniae a její funkcí
Pallová, Petra ; Přenosilová, Lenka ; Nováková, Linda ; Branny, Pavel
In order to determine a cellular localization of protein kinase StkP we prepared its epitope-labelled form expressed in S. pneumoniae. The full-length form of StkP was detected with monoclonal antibody in the membrane fraction. In vitro autophosphorylation assays confirmed this finding and phosphorylated product of corresponding mass was detected in the membranes. These results clearly showed that StkP is a membrane-associated protein.
Identifikace genů a proteinů, regulovaných Ser/Thr proteinkinasou Streptococcus pneumoniae, mikročipovou metodou a analýzou 2-D gelů
Přenosilová, Lenka ; Nováková, Linda ; Branny, Pavel
To identify the genes whose expression is controlled by protein kinase StkP we examined the transcription profile of DstkP mutant by DNA microarray technology. The transcript analysis revealed the StkP-dependent expression of many potential target loci. Out of 2,300 genes evaluated, 49 were found to be specifically and strongly upregulated in the DstkP strain, whereas 48 were downregulated. The data obtained from transcription profile were further extended by proteomic studies and mass spectrometry.
Ligninolytické enzymy rodu Trametes
Tomšovský, Michal ; Homolka, Ladislav
Eighty-four strains belonging to four species of the genus Trametes Fr. (T. versicolor, T. hirsuta, T. ochracea and T. cervina) collected in different localities and on different substrates were screened for laccase production and eight of them with their monokaryotic derivates were then examined in more detail for their ability to produce also other lignin-modifying enzymes (manganese peroxidase and lignin peroxidase). Production variability was relatively high and no significant correlation was found between the origin of the strains (locality, substrate) and the enzyme production. Dikaryons of all three species (but not of all their strains) exhibited LiP activity, which was not detected in the respective monokaryons.
Je EF-TU, jako dobře známý bakteriální molekulární přepínač, stále hádankou pro strukturní biology?
Holub, Martin ; Weiser, Jaroslav
We purified EF-Tu from two closely related strains of Streptomyces aureofaciens and used them in comparative studies in order to better understand the structural and functional features of their aggregation.
Rpg1p/eIF3a se spojuje s mitochondriemi v Saccharomyces cerevisiae
Hašek, Jiří ; Janatová, Ivana ; Špryngar, Martin ; Wolinski, H. ; Kohlwein, S. D.
RPG1/TIF32, the essential gene of Saccharomyces cerevisiae, encodes eIF3a subunit of the translation initiation factor 3. We show here that both temperature sensitive mutant of Rpg1 strongly differ in their distribution at permissive temperature, distribution of the Rpg1-1-GFP fusion is cytosolic, the fusion protein Rpg1-2-GFP was accumulated in large cytosolic aggregates. Using the life image analysis with confocal laser scanning microscope we found that these granules could be inherited to daughter cells and obviously associate with mitochondria.
Metodika navrhování řasových fotobioreaktorů: matematické modelování hydrodynamického míchání a předpověď parametrů světelného režimu
Papáček, Š. ; Rálek, P. ; Kopecký, Jiří ; Masojídek, Jiří ; Štys, Dalibor ; Petera, K.
Computational fluid dynamics (CFD) and process simulation are important tools for the design and optimisation of biochemical processes. In this contribution, the photobioreactor design methodology based on CFD is proposed. A widely used CFD package (Fluent 6.1, by Fluent Inc.) performs a numerical approximation of microalgae trajectory (pathline) inside tubular photobioreactor. Consequently, prediction of the level of hydrodynamic mixing and corresponding light regime inside the photobioreactor with effective use of strong light by high-density algal culture is possible. In the first approach, the algal suspension is considered as a single-phase incompressible viscous Newtonian fluid, which fills the whole section of the photobioreactor photic zone. Coupling the cell trajectories with the corresponding light conditions, the light histories of single cells are obtained. These records contain complete information about light regime in photobioreactor. Computer simulations for various photobioreactor design parameters and operating conditions allow to establish the quantitative relation between variables representing design and operating parameters and light regime parameters. Thus the problem of optimal PBR design could be resolved.
Dlouhodobé přežívání kolonií Saccharomyces cerevisiae: úloha transkripčního faktoru Sok2p
Váchová, Libuše ; Devaux, F. ; Kučerová, Helena ; Řičicová, M. ; Palková, Z.
Yeast colonies produce ammonia as a gaseous signal. Concomitantly, they lower stress factors and change the expression of genes encoding membrane proteins that transport ammonia (Ato proteins).
Synthetic Concepts a priori
Materna, Pavel
The rational core of Kant's claim that there are synthetic judgments a priori can be formulated as follows: some a priori (first of all mathematical) concepts cannot effectively determine their object.
Benchmarking in Science and Research
Filáček, Adolf
The contribution discusses selected methodology for benchmarking RTD organisations in Central and Eastern Europe, including the questionnaire used for verifying benchmarking indicators.
Kant and the Mainstream Ideology of the European Integration
Bednář, Miloslav
Kant's notion of republican globalization in incompatible with the federalist mainstream ideology of the current European Union.

Papers : 26,973 records found   beginprevious26954 - 26963next  jump to record:
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