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Rezistence genes against fungi deseases in cereals and methods for their detection
Stuchlíková, Šárka ; Dumalasová, Veronika (advisor) ; Schwarzerová, Kateřina (referee)
The paper deals with the possibilities of detection fungal disease resistance genes in the original European cereals, such as wheat, barley, rye and oats. It provides an overview of the most important resistance genes for breeding. The significance of the resistance genes is evaluated on the basis of the harmfulness of the individual cereal fungal diseases, as well as the importance of the individual cereal species. It describes the possibilities of detecting resistance genes using molecular markers and compares various types of molecular markers. It seeks answers to the question of where the breeding on resistance and therefore the use of molecular markers brings the greatest benefit.
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Development of molecular selection markers for detection of fertility restorer for CMS Shaan 2A
KARBANOVÁ, Aneta
This bachelor thesis deals with the development of molecular selection markers for the detection of fertility restorers for CMS Shaan 2A in rape by molecular techniques. The thesis describes molecular techniques such as DNA isolation techniques, polymerase chain reaction and primer design techniques for the detection of fertility restorers. Specific PCR primers for the Pentatricopeptide repeat (PPR) superfamily protein gene and the Tetratricopeptide repeat (TPR) -like superfamily protein gene sequence amplification have been designed and aplicons have been sequenced. On the basis of the sequence differences between plants with fertility restorer fenotype and CMS fenotype the technique for fertility restorer detection was designed.
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Molekulární markery pro detekci genetické variability přírodních populací forenzně významných druhů bzučivkovitých (Calliphoridae, Diptera)
KLOJDOVÁ, Martina
This thesis was focused on suitability of selected molecular markers for detection of genetic variability of four species from the family Calliphoridae important in the forensic entomology. A set of eight markers, both mitochondrial (COI, ND6, CytB, CR) and nuclear (RP S12, RP S13, RP L12, PB2), were applied on samples from natural populations of representative species (Calliphora vicina, Lucilia caesar, Lucilia sericata, Phormia regina) most common in the Czech Republic. Level of detected variability was evaluated and compared, both with respect to the particular species as well as their geographic origin.
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Dispersal of clonal aquatic plants
Rydlo, Jan ; Fér, Tomáš (advisor) ; Hroudová, Zdenka (referee)
The aim of this Bc. thesis is to summarize the knowledge on clonal dispersal of aquatic plants and to compare the possibilities and measure of clonal dispersal in selected most-studied species. In the first part of the study, ways of clonal propagation of water plants by various types of vegetative diaspores and various means of transport (by water, animals) are described. Ways and possibilities of dispersal of aquatic clonal plants in various water systems (river systems, stagnant waters) and to various distances are described as well. Finally, anthropogenic effects on clonal dispersal of water plants, which can also play an important role, are discussed. These effects could stay behind many invasive events on the Earth represented by rapid clonal reproduction and dispersal of some species of aquatic macrophytes in their non-native area. The next part of this thesis deals with methods to study the dispersal of aquatic clonal plants and assesses their relevance for various spatial scales. These methods and results of case studies are presented for the most often studied species of water macrophytes. Results of these studies are summarised to demonstrate the real efficiency and distances of clonal dispersal of aquatic plants. Powered by TCPDF (www.tcpdf.org)
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Genetic polymorphism in populations of Meligethes aeneus with different resistance to insecticides
WALTEROVÁ, Lucie
Thesis deals with pollen beetle populations with various degrees of resistance to pyrethroids. Samples were collected in 13 locations in Czech Republice in 2016. DNA of beetles was isolated by CTAB PVP and Chelex 100. After that, molecular methods based on ISSR markers (Inter-simple sequence repeats) were used to distinguish between different pollen beetle populations. Different populations were described based on microsatellites and the results were processed by the PCA analysis (Principal component analysis). 5 ISSR primers were tried and one result of the primers was reproducible. On the base of matrix genetic distance were samples classified to clusters according to genetic similar by PCA analysis. In the next step author evaluated relationship of geografical distance and different ISSR profile. In the end of the work, author tried to amplificate gene encoding voltage-sensitive sodium channels.
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Dispersion of freshwater gastropods
Buďová, Jana ; Černý, Martin (advisor) ; Juřičková, Lucie (referee)
Freshwater gastropods (Mollusca: Gastropoda) belong to two tradional taxonomic groups: prosobranchs (Prosobranchia) and pulmonates (Pulmonata). Most of these molluscs are characterized by low vagility. Therefore they usually rely on passive dispersal. Their dispersal vectors are mostly birds and water. Animals can transport snails both externally and internally. Direct methods (capture.mark.recapture, radio - tracking) and genetic methods could be used to study dispersal. For genetic studies can be used many type of molecular markers, but the recently most popular are microsatellites. According to recent studies, the dispersal of freshwater gastropods is probably not as frequent and wide - spread as assumed before. Key words: freshwater snails, dispersal, dispersal vectors, capture - mark - recapture, molecular markers, population genetics
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PCR-based detection of hidden carriers of cataracts in dogs
FARKOVÁ, Barbora
The hereditary cataract is one of the most common eye disease in dogs. The expansion of this disease in the Staffordshire bullterrier breed has been so massive that in the Czech Republic was introduced the rule of mandatory testing of at least one of a breeding pair. This is a degenerative disease of the lens causing total blindness of the affected animal within three years. Since some time ago there are no more dogs affected by the disease in the Czech Republic, there are however still hidden carriers which need to be discovered to the complete extinction of the disease in the genome. The goal of this study was to test simple ways of collecting biological samples, try them in practice and to verify whether they are suitable for the DNA isolation and also to test an alternative method of molecular detection of this disease. In total there have been 23 buccal swabs collected from male and female Staffordshire Bullterrier examples. The detection of the hidden carriers of the hereditary cataract was carried out by PCR analysis with specific primers. The obtained amplicons were detected by both gel and chip electrophoresis and by using fragment analysis. This detection of the carriers was based on the presence of two amplicons (heterozygotes). I came to conclusion that to detect hidden carriers it is neccessary to use the fragment analysis because of the difference of only one base in the reference section of DNA. Neither gel nor chip electrophoresis does provide sufficiently high resolution and it is not possible to detect two fragments that differ only by one bp. As the most appropriate sampling method I have chosen the buccal smear by cytological brush followed by isolating the DNA by Chelex with purification of the sample subsequently.
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Confirmation of \kur{Beauveria caledonica} occurence in Šumava National park by molecular markers
BINDER, Richard
Biological plant protection against insect pests is an important alternative to chemical protection. One of the most important group used in the biological plant protection against insect pests are the entomopathogenic fungi. Entomopathogenic fungi are microscopic fungi that are able to induce a primary disease to insect pests. It is a very heterogeneous group of species. Worldwide there were isolated and described more than 750 species of entomopathogenic fungi. Genus Beauveria is considered one of the most important genera of entomopathogenic fungi. In the Czech Republic there has been confirmed species B. bassiana, B. brongniartii and now, on the basis of this work, B. caledonica. This study is aimed to confirm the occurrence of B. caledonica in National Park Šumava. To confirm this occurrence, I used analyzes based on the methods of molecular markers. Molecular markers are an indispensable part of science in the field of mycology, for example the strain characterization, population genetics, detection and identification of fungi, phylogenetic studies and evolutionary biology. For this study there were used sequence analysis of ITS, EF1- and LSU regions. The output data of these analyzes were used to create phylogenetic trees. The result of my thesis is taxonomical classification of studied isolates on species level.
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