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Production of extracellular polysaccharides by extremophilic prokaryotes
Bystrická, Klaudia ; Smilek, Jiří (referee) ; Obruča, Stanislav (advisor)
Predložená diplomová práca sa zaoberá štúdiom biotechnologickej produkcie extracelulárnych polysacharidov (EPS) vybraných termofilných a halofilných mikroorganizmov. Po primárnom screeningu vybraných kultúr bola experimentálna časť tejto práce ďalej venovaná halofilnému archea Haloferax mediterranei. Cieľom tejto práce bola produkcia a charakterizácia polymérov a posúdenie biotechnologického potenciálu použitých extremofilných kultúr ako producentov EPS. Počas kultivácie H. mediterranei boli použité 3 základné média, ktoré boli následne optimalizované v prospech produkcie EPS. Získané precipitáty boli podrobené analýze pomocou GPC, FTIR a UHPLC-UV-ESI-MS/MS, na základe čoho bolo možné bližšie charakterizovať získaný produkt. Tieto experimenty dokázali, že so stúpajúcou koncentráciou NaCl v médiu, klesá výťažok polysacharidov a ich produkciu nepodporujú ani vysoké koncentrácie MgSO4 a KCl v médiu. Hlavnou komponentou polysacharidov vyprodukovaných H. mediterranei, je manóza, no v závislosti od použitého média môže obsahovať aj glukózu, galaktózu, ribózu alebo N-acetylglukosamin.
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Comparison of specific expression in bacterial and yeast biofilms
Kicko, Peter ; Palková, Zdena (advisor) ; Lichá, Irena (referee)
The development and maintenance of biofilm is a complex process that is based on a change in genetic expression. The biofilm formation is observed in some prokaryotic and eukaryotic cells. During its formation, cell aggregation occurs, extracellular matrix is created and we observe the formation of metabolically differentiable cells, often with increased resistance to antimicrobial drugs. This work focuses on important steps leading to biofilm formation associated with specific gene expression and highlights the similar and different processes between bacterial and yeast cells. The work begins by comparison of cell signalling, it continues by comparing the expression of the adhesive proteins and extracellular enzymes, synthesis of exopolysaccharides, formation of extracellular nucleic acid, and in the last chapter we focused on the formation of persistors. The aim of this work is to connect the acquired information and to contribute to the understanding the complexity of this process. Key words: biofilm, signalling, adhesins, exopolysaccharides, extracellular nucleic acid, persistor
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Comparison of specific expression in bacterial and yeast biofilms
Kicko, Peter ; Palková, Zdena (advisor) ; Lichá, Irena (referee)
The development and maintenance of biofilm is a complex process that is based on a change in genetic expression. The biofilm formation is observed in some prokaryotic and eukaryotic cells. During its formation, cell aggregation occurs, extracellular matrix is created and we observe the formation of metabolically differentiable cells, often with increased resistance to antimicrobial drugs. This work focuses on important steps leading to biofilm formation associated with specific gene expression and highlights the similar and different processes between bacterial and yeast cells. The work begins by comparison of cell signalling, it continues by comparing the expression of the adhesive proteins and extracellular enzymes, synthesis of exopolysaccharides, formation of extracellular nucleic acid, and in the last chapter we focused on the formation of persistors. The aim of this work is to connect the acquired information and to contribute to the understanding the complexity of this process. Key words: biofilm, signalling, adhesins, exopolysaccharides, extracellular nucleic acid, persistor
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