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DNA extraction from plant tissues for polymerase chain reaction analysis
Trojánek, Zdeněk ; RNDr.Roman Matyášek, CSc. (referee) ; Rittich, Bohuslav (advisor)
Extraction of nucleic acids is an important step for all molecular biological studies. The process of isolation of plant DNA is complicated due to the presence of polyphenols, polysaccharides and other metabolites. They can be co-isolated with DNA and act as PCR inhibitors. The aim of this study was to compare CTAB extraction procedure, Qiagen DNA easy kit, direct homogenization, carboxyl-functionalised magnetic non-porous HEMA based microspheres and combination of the above mentioned methods for DNA isolation from different plants. The DNA was evaluated regarding concentration, purity and amplification in PCR. All methods provided DNA that could be used in downstream PCR applications. However, there were differences regarding yield, purity, labour intensiveness and cost. Combination of direct homogenization and magnetic microspheres coated by carboxyl groups was isolated DNA from various plants and plant foods in a quality suitable for convectional PCR, real time PCR and restriction analysis. This method is fast, simple and does not require work with harmful substances.
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Kinds of transportation and impact on the enviroment
Trojánek, Zdeněk ; Mišík, Ondrej (referee) ; Prinz, František (advisor)
The bachelor thesis deals with transport and its impact on our surroundings, but also on our health. The main part of the work presents the characteristics of the mode of transport in terms of emissions and noise production. The resulting emissions are analyzed and the individual components are described in terms of their impact on nature and human health. The next part deals with possible ways to reduce emissions and noise and the different modes of transport are compared with each other.
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Cycle Product Management Study in the Enterprise
Dobešová, Lenka ; Trojánek, Zdeněk (referee) ; Jurová, Marie (advisor)
This thesis is focused on sustainable development of a company using PLM systém in the environment of an industrial company, that manufactures components for the automotive industry. In the theoretical part the life cycle of the product (PLM) is described as well as its demands and benefits. This is followed by an analysis of current IT environment, which contains basis for adjustments of the current system. Thank to them is possible to reduce narrow places of the slim production of the company, avoid wasting time and material, and above all to achieve sustainable development of the company while using current technologies.
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Identification and characterization of selected species of lactic acid bacteria
Trojánek, Zdeněk ; Špano,, Miroslav (referee) ; Rittich, Bohuslav (advisor)
Among the important representative of lactic acid bacteria are gram-positive bacteria of the genus Bifidobacterium. They are constituent part of ordinary intestinal flora, which help keep the balance and prevent colonization of intestinal by pathogens. Bifidobacteria are used in various dairy products for their prebiotic effect. Molecular biological methods are used for rapid and accurate identification of bacteria of genus Bifidobacterium. In our case, we have focused on DNA isolation and application of the polymerase chain reaction (PCR) for identification of bacteria of genus Bifidobacterium. Statistical evaluation of fingerprints obtained by rep-PCR with (GTG)5 primer was carrid out.
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Kinds of transportation and impact on the enviroment
Trojánek, Zdeněk ; Mišík, Ondrej (referee) ; Prinz, František (advisor)
The bachelor thesis deals with transport and its impact on our surroundings, but also on our health. The main part of the work presents the characteristics of the mode of transport in terms of emissions and noise production. The resulting emissions are analyzed and the individual components are described in terms of their impact on nature and human health. The next part deals with possible ways to reduce emissions and noise and the different modes of transport are compared with each other.
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|
|
Cycle Product Management Study in the Enterprise
Dobešová, Lenka ; Trojánek, Zdeněk (referee) ; Jurová, Marie (advisor)
This thesis is focused on sustainable development of a company using PLM systém in the environment of an industrial company, that manufactures components for the automotive industry. In the theoretical part the life cycle of the product (PLM) is described as well as its demands and benefits. This is followed by an analysis of current IT environment, which contains basis for adjustments of the current system. Thank to them is possible to reduce narrow places of the slim production of the company, avoid wasting time and material, and above all to achieve sustainable development of the company while using current technologies.
|
|
|
DNA extraction from plant tissues for polymerase chain reaction analysis
Trojánek, Zdeněk ; RNDr.Roman Matyášek, CSc. (referee) ; Rittich, Bohuslav (advisor)
Extraction of nucleic acids is an important step for all molecular biological studies. The process of isolation of plant DNA is complicated due to the presence of polyphenols, polysaccharides and other metabolites. They can be co-isolated with DNA and act as PCR inhibitors. The aim of this study was to compare CTAB extraction procedure, Qiagen DNA easy kit, direct homogenization, carboxyl-functionalised magnetic non-porous HEMA based microspheres and combination of the above mentioned methods for DNA isolation from different plants. The DNA was evaluated regarding concentration, purity and amplification in PCR. All methods provided DNA that could be used in downstream PCR applications. However, there were differences regarding yield, purity, labour intensiveness and cost. Combination of direct homogenization and magnetic microspheres coated by carboxyl groups was isolated DNA from various plants and plant foods in a quality suitable for convectional PCR, real time PCR and restriction analysis. This method is fast, simple and does not require work with harmful substances.
|
|
|
Identification and characterization of selected species of lactic acid bacteria
Trojánek, Zdeněk ; Špano,, Miroslav (referee) ; Rittich, Bohuslav (advisor)
Among the important representative of lactic acid bacteria are gram-positive bacteria of the genus Bifidobacterium. They are constituent part of ordinary intestinal flora, which help keep the balance and prevent colonization of intestinal by pathogens. Bifidobacteria are used in various dairy products for their prebiotic effect. Molecular biological methods are used for rapid and accurate identification of bacteria of genus Bifidobacterium. In our case, we have focused on DNA isolation and application of the polymerase chain reaction (PCR) for identification of bacteria of genus Bifidobacterium. Statistical evaluation of fingerprints obtained by rep-PCR with (GTG)5 primer was carrid out.
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