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Výšovice bypass - location study
Tichý, Václav ; Matuszková, Radka (referee) ; Radimský, Michal (advisor)
The aim of this bachelor thesis is to prepare a research study of a bypass of the Výšovice village. Výšovice is located in the Region of Olomouc, 7 km south of Prostějov. The bypass is designed as S7,5/90 and connects to the existing road II/433. Three options are proposed for the bypass, options are subsequently compared and one of them is elaborated in more detail. The construction of the bypass will reduce the traffic intensity in the village.
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The role of microRNA in hypoxic pulmonary hypertension
Tichý, Václav ; Hampl, Václav (advisor) ; Kolář, David (referee)
Pulmonary Arterial hypertension (PAH) is a devastating progressive disease that significantly decreases quality of life and has the average survival rate of only few years. One of the significant initiators of PAH is chronic hypoxia. After more than six decades of research that was initiated in 1946 by von Euler and Liljestrand, a new group of potential regulators of this pathology was discovered, that became heavily studied in the last five years. They are highly conserved molecules belonging to non-coding RNA. These 19-23 nucleotides long microRNA (miRNA) act as negative regulators of expression on various proteins. Many of them regulate traditional signalling pathways of hypoxic PAH (HIF-1, BMPR2) and miRNA is in turn regulated by other signalizations. Together, that creates an interconnected network of direct and indirect interactions and feedback loops, that we need to study in order to understand hypoxic PAH. This thesis summarizes findings about important miRNA molecules from the last few years and elucidates part of these regulatory mechanisms on several miRNA molecules (miR-17-92, miR-21, miR-210, miR-204 a miR143/145).
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Succinate dehydrogenase as a hypoxia sensor in pulmonary circulation
Tichý, Václav ; Hampl, Václav (advisor) ; Žurmanová, Jitka (referee)
Hypoxic pulmonary vasoconstriction (HPV) is a local physiological mechanism in lungs that optimalises blood oxygenation during alveolar hypoxia. Arterioles in the affected region increase flow resistance which redirects blood to better ventilated parts of the lung. During global hypoxia - e.g. in high altitude or in chronic pulmonary illnessess - this mechanism doesn't work, as the blood cannot be redirected elsewhere. The pressure in pulmonary artery rises which leads to right heart hypertrophy and ultimately to cor pulmonale. This mechanism has been studied for decades, but specific signalling pathways still lack full description and therapeutical solutions are not available. This thesis offers description of selected properties of pulmonary circulation and patophysiological context of pulmonary hypertension, introduces the reader to HPV localization and signalization, and discusses its most important steps from decreased oxygen availability to vessel constriction. The practical part of this work explores Succinate dehydrogenase (SDH) - complex coupling Kreb's cycle to electron transport chain - as a primary detection site of hypoxia in pulmonary artery smooth muscle cells. We decided to test this hypothesis in isolated rat lungs by measuring if malonate (SDH inhibitor) causes vasoconstriction as...
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The role of microRNA in hypoxic pulmonary hypertension
Tichý, Václav ; Hampl, Václav (advisor) ; Kolář, David (referee)
Pulmonary Arterial hypertension (PAH) is a devastating progressive disease that significantly decreases quality of life and has the average survival rate of only few years. One of the significant initiators of PAH is chronic hypoxia. After more than six decades of research that was initiated in 1946 by von Euler and Liljestrand, a new group of potential regulators of this pathology was discovered, that became heavily studied in the last five years. They are highly conserved molecules belonging to non-coding RNA. These 19-23 nucleotides long microRNA (miRNA) act as negative regulators of expression on various proteins. Many of them regulate traditional signalling pathways of hypoxic PAH (HIF-1, BMPR2) and miRNA is in turn regulated by other signalizations. Together, that creates an interconnected network of direct and indirect interactions and feedback loops, that we need to study in order to understand hypoxic PAH. This thesis summarizes findings about important miRNA molecules from the last few years and elucidates part of these regulatory mechanisms on several miRNA molecules (miR-17-92, miR-21, miR-210, miR-204 a miR143/145).
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