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Application of UHPLC with various types of detection in the field of environmental and food analysis.
Rejman, David ; Nováková, Lucie (advisor) ; Jáč, Pavel (referee)
The use of UHPLC with various types of detection in the field of environmental and food analysis Abstract The aim of this work was to inform about possible use of UHPLC instrumentation in connection with various types of detection when chromatographic separating of substances in the field of environmental and food analysis is performed. The next aim was to search and study a number of articles referring to UHPLC methodology, which are at present available in the internet databases ScienceDirect and Web of Science. On the basis of the information from the articles there is a graphic summary in this work relating to the number of found articles and the groups of analysed substances within years. In the introduction of the work van Deemter's, Gidding's and Knox's theories about the efficiency of the chromatographic system are mentioned. These theories are substantial for understanding the principle of chromatographic separation. Following chapters deal with the problems of the development of chromatography in the last decades, especially concerning the length and type of analytical columns, the size of filling particles or the temperature and pressure in the chromatographic system. Now thanks to the development, the columns dimensions are 50 x 1 mm and are filled with particles less than 2 μm in diameter and...
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Application of UHPLC with various types of detection in the field of environmental and food analysis.
Rejman, David ; Jáč, Pavel (referee) ; Nováková, Lucie (advisor)
The use of UHPLC with various types of detection in the field of environmental and food analysis Abstract The aim of this work was to inform about possible use of UHPLC instrumentation in connection with various types of detection when chromatographic separating of substances in the field of environmental and food analysis is performed. The next aim was to search and study a number of articles referring to UHPLC methodology, which are at present available in the internet databases ScienceDirect and Web of Science. On the basis of the information from the articles there is a graphic summary in this work relating to the number of found articles and the groups of analysed substances within years. In the introduction of the work van Deemter's, Gidding's and Knox's theories about the efficiency of the chromatographic system are mentioned. These theories are substantial for understanding the principle of chromatographic separation. Following chapters deal with the problems of the development of chromatography in the last decades, especially concerning the length and type of analytical columns, the size of filling particles or the temperature and pressure in the chromatographic system. Now thanks to the development, the columns dimensions are 50 x 1 mm and are filled with particles less than 2 μm in diameter and...
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