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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Preparation of HEK293 cell line expressing auxin transporter PIN7 and testing of inhibitors of auxin transport
 Petermannová, Romana ; Vaněk, Ondřej (advisor) ; Moserová, Michaela (referee)
Auxin is one of the most important plant hormones, which provides development of a plant. PIN1 and PIN7 proteins belong to the PIN family of transporters which is among the most important auxin efflux carriers. This thesis deals with the of AtPIN1 and AtPIN7 auxin efflux carriers (from Arabidopsis thaliana) in human embryonic kidney 293 cell line. Biological activity of these proteins was tested by using radiolabeled auxins accumulation. Further inhibitors of auxin transport have been tested - NPA, CHPAA and BFA.
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Production of auxin transporter PIN1 in HEK293 cell line
 Petermannová, Romana ; Vaněk, Ondřej (advisor) ; Hýsková, Veronika (referee)
PIN1 protein belongs to a PIN family of transporters. This group of transporters is significantly involved in auxin transports outside of the cell. The auxin transport among plant cells gives an important role in plant growth, gravitropism, etc. The principle of auxin transport by this carrier protein is still not explored. This work deals with the production of PIN1 carrier protein by recombinant expression in human embryonic kidney 293 cell line. Production in these cells was checked by the accumulation of radioactive auxin and by the immunofluorescence.
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