|
| |
|
|
Autonomous system for maintaining the biophysical conditions required for culturing biological cultures in vitro
Matějka, Roman ; Rosina, Jozef (advisor) ; Pantoflíček, Tomáš (referee) ; Kvašňák, Eugen (referee)
Cardiovascular disease is one of the leading causes of death worldwide. Surgical techniques, including bridging, replacing, or plasticizing damaged tissue, need suitable grafts for these operations. The ideal source is autologously obtained grafts, which, however, require another invasive approach, and there are not enough of them, or they do not meet the optimal anatomical form. Allogeneic grafts from cadaveric donors are not immediately available for acute interventions. Synthetic replacements show good results in large arteries, but restenosis occurs in small diameter vessels and often require further reoperation. The use of tissue engineering methods to modify these replacements by cell colonization may provide their better patency. Furthermore, decellularized xenogeneic tissues are promising matrices for the development of tissue-engineered cardiovascular grafts. Due decellularization the immunogenic complex is minimized while maintaining a suitable building structure. In combination with recolonization with suitable cells using culture bioreactors, new functional cardiovascular replacements can be created. Endothelialization of the inner lumen is a crucial element in the formation of cardiovascular replacements. The effect of shear stress simulated in the bioreactor has a positive effect on...
|
|
|
Liver regeneration after partial hepatectomy in rats suffering from non-alcoholic fatty liver disease
Garnol, Tomáš ; Červinková, Zuzana (advisor) ; Pantoflíček, Tomáš (referee) ; Šiller, Jiří (referee)
1. SUMMARY Liver regeneration after partial hepatectomy in rats suffering from non-alcoholic fatty liver disease Non-alcoholic fatty liver disease (NAFLD) is currently the most frequent chronic liver disease in economically developed countries with prevalence of about 30 %. Liver resection from different reasons is nowadays common surgical procedure. Successful recovery and renewal of liver functions depend on regenerative capacity of liver remnant. The course of liver regeneration could be profoundly influenced by concomitant liver pathological processes including NAFLD. The aim of this work has been study of early phase of liver regeneration after partial hepatectomy (PHx) in rats with nutritionally induced simple steatosis. At the beginning we introduced a model of NAFLD by a high-fat diet giving to Wistar or Sprague-Dawley rats for 3 and 6 weeks, resp. This regimen induced simple steatosis without signs of inflammation, necrosis or fibrotic changes in both strains. In the second part of our study we followed if liver regeneration after PHx in rats with NAFLD is altered. Liver regeneration was assessed by bromodeoxyuridin incorporation into DNA of hepatocytes. Regeneration of liver with simple steatosis induced by 2/3 PHx was not significantly influenced in comparison to non-steatotic liver. The only...
|
|
|
Bioartificial liver in the treatment of experimental acute hepatic failure in a large laboratory animal
Pantoflíček, Tomáš ; Šmejkal, Karel (advisor) ; Král, Vladimír (referee) ; Třeška, Vladislav (referee)
Bioartificial liver in the treatment of experimental acute hepatic failure in a large laboratory animal Aim: To evaluate bioartificial liver (BAL) in the treatment of acute liver failure in the experiment. Methods: 10 minipigs weight 25 - 30 kilograms with experimental acute liver failure (ALF) by surgical devascularization with portocaval by-pass were treated by BAL. The biochemical and hemodynamical parameters of ALF including intracranial pressure (ICP) were measured. The results were compared to control group without BAL treatment. Results: To compare plasma bilirubin there was statistically significant difference between experimental and control groups in 6th and 9th hours to favourite BAL group of minipigs (20,86 vs. 10,60, 22.29 vs. 11,75) and in the 12th hour (37,4 vs. 12,88). The value of ICP in both groups was not significant. Conclusion: The functionality of BAL on our own surgical model of ALF was proven. Initial 85 % viability of fresh isolated porcine hepatocytes and their survival in the time of BAL treatment was reached. Bilirubin plasma concentration has significantly changed in comparison in both groups. ICP values were not changed significantly. Key words: acute liver failure - minipig - bioartificial liver - BAL - Bioreactor - hepatocytes - intracranial pressure
|
|
| |
|
| |
guest ::
