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Model of cerebral focal cortical ischemia and its parametrization
Svoboda, Jan ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
Title of work: Model of cerebral focal cortical ischemia and its parametrization Work objectives: The aim of this diploma thesis was to apply modified model of focal cortical brain ischemia induced by phototrombosis and subsequently determinate its parameters. Methods: Intravenous application of photosensitive Rose Bengal dye was followed by continual illumination of green laser beam over the left sensorimotocortex for 10 minutes. Following illumination, the dye is activated and produces singlet oxygen that damages components of endothelial cell membranes, with subsequent platelet aggregation and thrombin formation, which eventually determines the interruption of local blood flow. This approach, initially proposed by Rosenblum and El-Sabban in 1977, was later improved by Watson in 1985 in rat brain. For histological evaluation of ischemic brain damage, animals were overdosed with urethane and transcardially perfused. Results: Histological examination of brains showed significant ischemic damage in all experimental animals. Lesion was located in left hemisphere and penetrated thought the grey matter in various extents. Size of lesion, its localization and depth has shown only a small variability in the individual groups. Noticeable differences were found right after comparing experimental groups....
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Modelling of changes of the intracranial pressure (ICP) during physiological and pathological processes
Vaněk, Petr ; Otáhal, Jakub (advisor) ; Suchomel, Petr (referee) ; Sameš, Martin (referee)
The more we know about the circulation of CSF through ventricle system, brain parenchyma, subarachnoideal space and the development of intracranial pressure (ICP), the more we understand different pathologies and pathological processes of the central cervous system. The knowledge about the basic hydrodynamic charecteristics of craniospinal system is a key factor for understanding a wide range of pathological situations affecting CNS, these are for example intracranial hypertension, normal pressure hydrocephalus and syringomyelia. In the presented paper, we are presenting a newly developed measuring system Visionbrain that enables us to gain the biological data and consequently to analyse them. At the same time, the results of such measurements were used to specify the compertmental model of CSF circulation. The model devides craniospinal system into five divisions - two intracranial and three spinal. Such model is the third generation of this model and, compared to the older version, it includes two intracranial veins. Thanks to this stucture, the model enables us simultaneous modeling of heart and respiratory pulsations of CSF. Compared to the other published models, it also includes defined structure of spinal part. The model is derived using laws of conservation and it encompasses pressure...
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Influence of crank arm length on change of muscle tone of musculus triceps surae
Stránský, Vojtěch ; Šifta, Petr (advisor) ; Otáhal, Jakub (referee)
Title: Influence of crank arm length on change of muscle tone of musculus triceps surae Objectives: The aim of this thesis is to conclude the theoretical knowledge of this issue. The experiment is to uncover via the objective results, if the change of the crank arm length can influence the muscle tone of m.triceps surae and additionaly how much. Methods: The measurement for this thesis was processed at Charles University at the Faculty of Physical Activity and Sports, measuring 8 volunteers. Via myotonometry, the biomechanic properties (stiffness and viscosity) of m.triceps surae were measured before and after the workout on a bicycle ergometer. These steps were repeated twice within a week with different crank arm length (170mm and 175mm). On the basis of the reaction of soft tissues on deformation from the tip of the myotonometer, the graphs are depicted, containing the hysteresis curve. We evaluate there two phenomenons - steepness and the content of the curves. They indicate biomechanic properties of soft tissues, resp. also the muscle tissue. Results: The measurements from the 1st and 2nd week were compared. The results of 8 probands show that cycling as a physical activity changes stiffness and viscosity of m. triceps surae and has therefore an influence on the muscle tone. Considering larger...
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Consequences of experimentaly induced perinatal cerebral ischemic lesion
Bahníková, Eva ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
1 Abstract Title: Consequences of experimentally induced perinatal cerebral ischemic lesion. Aim: This thesis aims to present the issues and analyze current knowledge in the field of perinatal brain damage, particularly perinatal cerebral ischemic lesions as the most common brain infarction in children. Basic characteristics of the disease, syndromology, pathophysiological mechanisms and risk factors are emphasized. Following the theoretical background the thesis analyzes current trends as well as the limitations of the diagnostics and therapy. The aim is to highlight the need for early diagnosis and emphasize the potential of preventive treatment strategies. The practical part follows the theoretical background and expands the topic on the analysis of motor and behavioral consequences of experimentally induced perinatal ischemic stroke. Method: Cerebral focal ischemia was induced experimentally by photothrombotic method to seven days old rats. Rose Bengal intravenously followed by continual illumination of the senzorimotor cortex using a green laser beam for 10 minutes was induced platelet aggregation and subsequent thrombosis. Eight animals at age of two months were evaluated for 7 days via observational cages PhenoTyper. Results: The theoretical part collected and processed theoretical data on the...
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Acute neuroinflammatory reaction after focal cerebral ischemia
Ambrož, Ondřej ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
Title: Acute neuroinflammatory reaction after focal cerebral ischemia Aim: The aim of this thesis is to evaluate neuroinflammatory response after focal cerebral cortical ischemia. Also, familiarizing with the method of displaying damage of blood brain barriers, neurons and the possibility of detection of microglia cells as a marker of acute neuroinflammatory processes. Methodology: This is an experimental study. We brought about cortical cerebral ischemia in rats using an application of photosensitive dye "bengal red," and a green laser. Two animals were were given the additional application of "Evans blue" in order to visualize the defects of the blood brain barrier. The animals were returned to their cage for the time needed before they were induced terminal anesthesia. This was followed by the process of brain perfusion, slicing the brain in sections 50 µm thick and then applied these sections onto slides. Sections with applied EB were immediately analyzed under the microscope. Sections to illustrate neuronal death were immunohistochemically stained via the Nissl method. Sections visualizing microglial activity were stained using CD11b antibody. Results: Following the induction of focal ischemia there occured brain tissue damage. In the vicinity of lesion there is degeneration of neurons and...
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The influence of bicycle saddle position on muscle tone of the triceps surae muscle
Králíčková, Iva ; Šifta, Petr (advisor) ; Otáhal, Jakub (referee)
Title: The influence of bicycle saddle position on muscle tone of the triceps surae muscle Objectives: The aim of this thesis is to find out what is the effect of changing the bicycle saddle position on muscle tone of triceps surae muscle. And whether the higher seat position will also increase muscle tone of the calf muscle. Methods: The thesis is divided into the theoretical part, where there is the information from the scientific literature on the subject and a practical part. The theoretical part includes anatomical descriptions and rheological properties of skeletal muscles, muscle tension and its regulation. Part of it is also the biomechanics of cycling, bike geometry and the options of bicycle seat setting and also the risk implications of the incorrect bicycle seat position. The practical part was prepared as a research by measuring of muscle tone by the myotonometer. For measurement was used the triceps surae muscle of the seven probands on the dominant lower limb. The measurement were twice two weeks apart with the different bicycle seat height. Results: The measurement results confirmed that change of bicycle saddle position has an influence on the muscle tone and that the higher saddle position will also increase muscle tone of the calf muscle. Keywords: bicycle saddle position, saddle...
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Stretching and the Maximal Muscle Strength
Šádek, Petr ; Konopková, Renata (advisor) ; Otáhal, Jakub (referee)
Title: The effect of stretching on maximal muscle performance Objectives: Due to the number of studies, concerning the effect of the muscle stretch on sport performance and their heterogeneity in the term of used parameters, tests and even performing the stretch exercises themselves, it would be desirable, thanks to the importance of this subject, to consolide and evaluate up-to-date knowledge. The goal of this diploma thesis is to compare and assess available literature concerning the effect of muscle stretch during warmup on the sport performance and, ideally, to define the best type of the stretching itself. Method: The diploma thesis is composed as a literature review - an integrative review with exactly defined criteria for the use of available studies. Result : Even with the nonuniformity of parameters used in available studies there is a consensus in most of the used sources, which met the criteria of inclusion in this research, which (the consensus) determinates the dynamic stretching as the form most appropriate for enhancing the following sport performance. Although studies, proving this result, are in their findings quite unanimous, a precise definition of prefered exercises for peforming the dynamic stretching and of its parameters is still missing. Key words: stretching, dynamic,...
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Golf swing in subjects with amputation of lower extremity
Richtrová, Michaela ; Otáhal, Jakub (advisor) ; Šifta, Petr (referee)
Author: Bc. Michaela Richtrová Tittle: Golf swing in subjects with amputation of lower extremity Objectives: The study aims at evaluation of the technique of golf swing used by patients with a different type and level of lower extremity amputation as compared to able-bodied golfers. Another objective is determination of the relation between individual phases of the golf swing and weight transfer, using different golf clubs, as compared to able-bodied golfers. Method: By its topic the work is a pilot study. The research has been divided into three descriptive case studies. The experimental group included two amateur golfers and one professional golfer. The control group was represented by an able-bodied golfer. Evaluation of kinematic parameters (trajectory of shoulders, hips, knee joints) was based on the Qualysis Motion Capture System. Evaluation of dynamic parameters (weight transfer) in the course of golf swing was made using two dynamometric Kistler platforms. Results: The measuring has demonstrated that in terms of both kinematics and dynamics, the mode of movement of a patient with a trans-tibial amputation during a golf swing corresponds to that of an able-bodied golfer. The same results were achieved by the patient also with the use of another type of golf club. A change, both in terms of...
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The role of nitric oxide in the patophysiology of neurodegenerative diseases
Matušková, Hana ; Otáhal, Jakub (advisor) ; Alánová, Petra (referee)
The bachelor thesis deals with the importance of nitric oxide in the development of neurodegenerative diseases. Nitric oxide is a biological active gas which affects, among other things, many functions of the central nervous system. Its activity is dependent on the concentration in small amounts has a positive effect, while overproduction contributes significantly to the development of neurodegenerative diseases. In my work, I deal with the negative influence of nitric oxide in the development of epileptic seizures, Parkinson's disease, Alzheimer's disease and Huntington's disease. In conclusion, I mentioned the possibility of determining the amount of nitric oxide concentration. Keywords Nitric oxide, ROS, RNS, neurodegenerative diseases, epileptic seizures, Parkinson's disease, Alzheimer's disease, Huntington's disease
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Recruitment curve of H reflex in diagnostics of pseudoradicular syndrom
Vocilka, Jindřich ; Otáhal, Jakub (advisor) ; Šifta, Petr (referee)
Title: Recruitment curve of H-reflex in diagnostics of non-radicular syndroms Objectives: The main objective of this work is to verify the conductivity of nerve structures examination recruitment curves H-reflex and M-wave in the EMG examination in probands with non-radicular syndrome L5/S1. Methods: We tested 12 probands clinical trial diagnosed with non-radicular syndrome L5/S1. The test sample was compared with an equally large group of control probands. H-reflex of m.soleus was examined by bipolar stimulation in the fossa poplitea of tibial nerve. The results of an electromyographic examination was evaluated to asymptomatic and symptomatic side. Amplitude of H-reflex and M-wave, slope of recruitment curve, peaks of recruitment curves for the H-reflex and M-wave and the ratio Hmax / Mmax were evaluated and statistically compared. Results: Results of non-radicular syndrome L5/S1showed no significant change in the H-reflex. Due to this result we can suggests that there is no damage in nerve fibres in patients with non-radicular syndrome. Keywords: non-radicular syndrome, H-reflex, M-wave, recruitment curve
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