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Molecular cloning of YinP gene from Leishmania major using two red fluorescent pXG-mCherry plasmids. Valuable tools for gene expression location.
Musilová, Kateřina ; Wsól, Vladimír (advisor) ; Štambergová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kateřina Musilová Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Tutor: Prof. Paul Nguewa, Ph.D. Title of diploma thesis: Molecular cloning of YinP gene from Leishmania major using two red fluorescent pXG-mCherry plasmids. Valuable tools for gene expression location. In the 21st century, leishmaniasis remains a major health problem in numerous de- veloping countries. Around 2 million cases of leishmaniasis are reported every year and estimated mortality is over 20,000 deaths annually. Antileishmanial drugs are often unaf- fordable for affected people and display severe toxic side effects. Potent human vaccines are not available. This, together with increasing resistance, is a reason why new effective, safe, and affordable medicines are greatly needed. Leishmaniasis is caused by Leishmania species. These parasites are transmitted by phlebotomine sand flies, which also provide to the leishmania environment necessary for their development into infective forms. The process of transformation into a stage in- fective for vertebrate hosts is called metacyclogenesis. Nowadays, genes, enzymes, and proteins possibly exhibiting a function in the metacyclogenesis are extensively examined. One of the...
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Stress-strain analysis of lower limb prosthetic
Musilová, Kateřina ; Fuis, Vladimír (referee) ; Návrat, Tomáš (advisor)
This master thesis deals with lower-limb prosthesis. The aim of the first part of this work is stress-strain analysis of trans-tibial prosthesis, which is understand without prosthetic socket and the foam prosthetic feet. Analysis is made using finite element method in ANSYS Workbench 12.0 software. For the purpose of setting up the computational model it is necessary to make few partial models. Model of geometry of the prosthesis is made in SolidWorks 2009. Based on the outputs of stress-strain analysis and the results evaluation, the critical component is chosen and this one is evaluated according to limite state for fatigue. The algorithm of evaluation of named component in the state of high cycle fatigue is discussed in the second part of this thesis.
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Scientific technology park Telč
Musilová, Kateřina ; Suchánek,, Petr (referee) ; Nový, Alois (advisor) ; Donaťáková, Dagmar (advisor)
The subject of bachelor thesis is a design of a building of a Scientific Technology Park with laboratories for Energoklastr in Telč. Energoklastr is competency oriented cluster focusing on co-operation of small and medium size businesses, research organizations, universities and public sector in field of applied research and transfer of technologies. It significantly contributes to development of innovations, applied research and transfer of technologies in field of lowering energy demand. Energoklastr especially focuses its activity on technology lowering emission levels and sustainability of energetics in fields like a production of electric devices, aerial industry, car industry, IT and construction. Building site for meant for the construction of VTP with laboratories of effective use of biomass in energetics is to be found in cadastral area of Telč. It is located in north-west part of town, in neighborhood of existing industrial and agricultural buildings. The property is adjacent to a first class road number 23 (Třebíč – Jindřichův Hradec), that is one of the main connection to highway D1. VTP building with the laboratory is designed like a two story wooden structure with a flat roof and no cellar. The building is trapezoidal shaped, which is adjusted to a shape of the property. The building is divided to laboratory part and administrative part according to a story. There is a suspension stainless steel staircase designed in a lobby, which is discussed in part D – Architectonical detail.
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Filling the needs of selected clients living in homes for the elderly with an emphasis on sexuality
MUSILOVÁ, Kateřina
This thesis deals with the filling of selected customer. Leeds for the elderly with an emphasis on sexuality. I evaluated primarily its fulfillment, mening, and the wiew of social workers on the subjekt. In the theoretical part I focused on aging, age, health ganges, health ganges that affesct sexual life and the very elderly sexuality. I also tried find signifiant determinants that affect social workers views on sexuality seniors and also on the way to the fulfillment of its. Professional in care homos for the elderly. In the research I examined the communication of caring in connection with the intimate life of seniors. The work is focused on the analysis of the results obtained exploratory investigation.
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Appraisal of MS KART s.r.o.
Musilová, Kateřina ; Mařík, Miloš (advisor) ; Mareška, Zdeněk (referee)
The subject of this diploma thesis is the appraisal of the MS KART s.r.o., as of 1. 1. 2011 for the internal purposes of the ordering party, which is the appraised company. MS KART s.r.o. operates as a producer within the carting industry. The thesis is based on four main parts. Financial analysis is aimed at examining the company's financial health. Strategic analysis defines and evaluates the relevant market in which the company operates and it also quantifies the potential of the company to succeed. Executing both analyses provides the answer about whether the company fulfills the going concern requirement or not. The third portion assesses the main figures which determine the value of the company. Based upon these assessments a financial plan is constructed. In the final part, the company is appraised by using two methods - the amortization value method and the liquidation value method. Their results represent the final interval of the appraisal of the company. The appraisal is calculated in terms of equity appraisal.
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