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National Repository of Grey Literature

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	Plasmide DNA isolation from bacteria and transfection to HEK293 cell line Měsíčková, Klára ; Fohlerová, Zdenka (referee) ; Svoboda, Ondřej (advisor) The isolation of plasmid DNA is an important and often used method in microbiology. The isolation itself is preceded by preparation of bacterial competent cells and by amplification of the plasmids. In this stage, plasmids CHR2, ASAP1, ASAP-3, ASAP-5 and Kir2.1. are first amplified in E.Coli bacteria of the DH5 strain and then isolated through the method of phenol-chloroform extraction. Gel electrophoresis and transfection to cellular line HEK293 are used for determining the correctness of the isolation. Detailed record
	Electrophysiological characterization of Kir2.1 membrane channel Měsíčková, Klára ; Chmelíková, Larisa (referee) ; Svoboda, Ondřej (advisor) The topic of this thesis is electrophysiological characterization of Kir2.1 membrane channel. Inward rectifier potassium channel Kir2.1 is located in muscular, heart and nerve cells and its dysfunction causes various diseases. Practical part of this stage is focused on cultivation of the HEK293T cell line that is used to transfection of the plasmid Kir2.1 and subsequent measurement of the ionic current through the electrophysiological method patch-clamp in whole-cell mode. Detailed record
	Analysis of the characteristics of QRS complex in normal and high-frequency electrocardiogram recording Měsíčková, Klára ; Ronzhina, Marina (referee) ; Kozumplík, Jiří (advisor) This work introduces and evaluates methods for normal and high-frequency electrocardiogram depending on the potential clinical use for the evaluation of abnormalities in cardiac ischemia. The first part is devoted to the creation and scanning cardiac activity. Subsequently are described in the text abnormality ST segment and QRS complex in the low-and high-frequency electrocardiogram. In the second part of the statistical processing of data of healthy subjects and patiens and compared the results for different frequency bands. Detailed record
	Electrophysiological characterization of Kir2.1 membrane channel Měsíčková, Klára ; Chmelíková, Larisa (referee) ; Svoboda, Ondřej (advisor) The topic of this thesis is electrophysiological characterization of Kir2.1 membrane channel. Inward rectifier potassium channel Kir2.1 is located in muscular, heart and nerve cells and its dysfunction causes various diseases. Practical part of this stage is focused on cultivation of the HEK293T cell line that is used to transfection of the plasmid Kir2.1 and subsequent measurement of the ionic current through the electrophysiological method patch-clamp in whole-cell mode. Detailed record
	Plasmide DNA isolation from bacteria and transfection to HEK293 cell line Měsíčková, Klára ; Fohlerová, Zdenka (referee) ; Svoboda, Ondřej (advisor) The isolation of plasmid DNA is an important and often used method in microbiology. The isolation itself is preceded by preparation of bacterial competent cells and by amplification of the plasmids. In this stage, plasmids CHR2, ASAP1, ASAP-3, ASAP-5 and Kir2.1. are first amplified in E.Coli bacteria of the DH5 strain and then isolated through the method of phenol-chloroform extraction. Gel electrophoresis and transfection to cellular line HEK293 are used for determining the correctness of the isolation. Detailed record
	Analysis of the characteristics of QRS complex in normal and high-frequency electrocardiogram recording Měsíčková, Klára ; Ronzhina, Marina (referee) ; Kozumplík, Jiří (advisor) This work introduces and evaluates methods for normal and high-frequency electrocardiogram depending on the potential clinical use for the evaluation of abnormalities in cardiac ischemia. The first part is devoted to the creation and scanning cardiac activity. Subsequently are described in the text abnormality ST segment and QRS complex in the low-and high-frequency electrocardiogram. In the second part of the statistical processing of data of healthy subjects and patiens and compared the results for different frequency bands. Detailed record

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1	Měsíčková, Kristýna

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