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Early craniofacial and dental morphogenesis in bichir, P. senegalus
Kráľovič, Martin ; Černý, Robert (advisor) ; Buchtová, Marcela (referee)
Polypterid fishes are considered the basal-most group of extant actinopterygians and thus may serve as a direct link for understanding the evolution of the first bony fishes. Their embryonic and larval material, however, is extremely scarce what makes it difficult to study their developmental patterns and processes. This work of mine is based on several successful breedings of the bichir Polypterus senegalus that allowed me to analyse development of some 70 specimens at key developmental stages. I have focused my attention to the early morphogenesis of teeth and dentition on several locations within a developmental context of oropharyngeal cavity. Oropharyngeal cavity develops quite differently when compared to other vertebrate species: it arises by means of a schizocoeliclike split of epithelial layers and, moreover, the earliest mouth cavity seems to be strongly constrained by the presence of cement (attachment) glands. Mouth opening proceeds by development of new, enigmatic structures that I have entitled the epithelial bridges (Kralovic et al., 2009) and that might represent the ectoderm-endoderm border often judged to be essential for tooth initiation and patterning. I have described a pronounced delay in initiation of pharyngeal teeth when compared to the oral teeth, probably as a direct consequence...
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Functionalized nanofiber system for accelerated regeneration of internal tissues
Kráľovič, Martin ; Amler, Evžen (advisor) ; Gášková, Dana (referee) ; Chvojka, Jiří (referee)
Functionalized nanofiber system for accelerated regeneration of internal tissues Abstract Functionalization can adjust the properties of nanofibers prepared by electrospinning to best replace the missing extracellular matrix in the healing wound. In the rabbit intestinal anastomoses, functionalization of nanofibers by cryogenic fractionation has proven to be crucial. While the use of functionalized PVA and chitosan nanofiber membranes led to intestinal strictures and severe inflammation, further functionalization of PVA and chitosan nanofibers by their cryogenic fractionation led to the possibility of reducing the polymer dose, which significantly reduced the inflammatory response and significantly increased the biocompatibility of the material used. The application of fractionated nanofibers increased the fraction of microvessels, fibroblasts and collagen, which resulted in an increase in the biomechanical strength of healed anastomoses. The functionalization of the polycaprolactone nanofibers by special adhesion to the polypropylene mesh made it possible to use them as a support for the healing of the fascia in the abdominal wall of pigs. Functionalization of PVA nanofibers with glyoxal led to their increased stability in the aqueous environment. By applying a membrane of PVA functionalized nanofibers to...
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Early craniofacial and dental morphogenesis in bichir, P. senegalus
Kráľovič, Martin ; Černý, Robert (advisor)
Polypterid fishes are considered the basal-most group of extant actinopterygians and thus may serve as a direct link for understanding the evolution of the first bony fishes. Their embryonic and larval material, however, is extremely scarce what makes it difficult to study their developmental patterns and processes. This work of mine is based on several successful breedings of the bichir Polypterus senegalus that allowed me to analyse development of some 70 specimens at key developmental stages. I have focused my attention to the early morphogenesis of teeth and dentition on several locations within a developmental context of oropharyngeal cavity. Oropharyngeal cavity develops quite differently when compared to other vertebrate species: it arises by means of a schizocoelic- like split of epithelial layers and, moreover, the earliest mouth cavity seems to be strongly constrained by the presence of cement (attachment) glands. Mouth opening proceeds by development of new, enigmatic structures that I have entitled the epithelial bridges (Kralovic et al., 2009) and that might represent the ectoderm-endoderm border often judged to be essential for tooth initiation and patterning. I have described a pronounced delay in initiation of pharyngeal teeth when compared to the oral teeth, probably as a direct...
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Early craniofacial and dental morphogenesis in bichir, P. senegalus
Kráľovič, Martin ; Černý, Robert (advisor)
Polypterid fishes are considered the basal-most group of extant actinopterygians and thus may serve as a direct link for understanding the evolution of the first bony fishes. Their embryonic and larval material, however, is extremely scarce what makes it difficult to study their developmental patterns and processes. This work of mine is based on several successful breedings of the bichir Polypterus senegalus that allowed me to analyse development of some 70 specimens at key developmental stages. I have focused my attention to the early morphogenesis of teeth and dentition on several locations within a developmental context of oropharyngeal cavity. Oropharyngeal cavity develops quite differently when compared to other vertebrate species: it arises by means of a schizocoelic- like split of epithelial layers and, moreover, the earliest mouth cavity seems to be strongly constrained by the presence of cement (attachment) glands. Mouth opening proceeds by development of new, enigmatic structures that I have entitled the epithelial bridges (Kralovic et al., 2009) and that might represent the ectoderm-endoderm border often judged to be essential for tooth initiation and patterning. I have described a pronounced delay in initiation of pharyngeal teeth when compared to the oral teeth, probably as a direct...
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Early craniofacial and dental morphogenesis in bichir, P. senegalus
Kráľovič, Martin ; Buchtová, Marcela (referee) ; Černý, Robert (advisor)
Polypterid fishes are considered the basal-most group of extant actinopterygians and thus may serve as a direct link for understanding the evolution of the first bony fishes. Their embryonic and larval material, however, is extremely scarce what makes it difficult to study their developmental patterns and processes. This work of mine is based on several successful breedings of the bichir Polypterus senegalus that allowed me to analyse development of some 70 specimens at key developmental stages. I have focused my attention to the early morphogenesis of teeth and dentition on several locations within a developmental context of oropharyngeal cavity. Oropharyngeal cavity develops quite differently when compared to other vertebrate species: it arises by means of a schizocoeliclike split of epithelial layers and, moreover, the earliest mouth cavity seems to be strongly constrained by the presence of cement (attachment) glands. Mouth opening proceeds by development of new, enigmatic structures that I have entitled the epithelial bridges (Kralovic et al., 2009) and that might represent the ectoderm-endoderm border often judged to be essential for tooth initiation and patterning. I have described a pronounced delay in initiation of pharyngeal teeth when compared to the oral teeth, probably as a direct consequence...
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