|
|
The effect of polyketide antibiotics on signalling and functional activity of human monocytic cell line
Kopecká, Kristýna ; Stříž, Ilja (advisor) ; Kovářová, Jaromíra (referee)
Anti-inflammatory cytokines have an important role in the development of inflammatory reactions. If an acute inflammation turns into chronical it is very often a pathological phenomenon. Chronicle inflammations accompany a whole number of serious diseases with an unclear prognosis, such as some of the autoimmune diseases. Usually, the cause of these diseases is not quite clear and the treatment is mainly symptomatic with an effort to suppress the immunity system. For this purpose we use various immunosuppressant drugs, and biological treatment is used, too. Another possibility is to use bioactive secondary metabolites produced by various microorganisms. In this group there are for example macrolides antibiotics, and a big potential is also seen in the recently discovered polyketides. The objective of this work is to test the newly acquired secondary metabolites that were isolated in the Laboratory of Molecular Biology of Actinomycetes at the Czech Academy of Sciences. Tested were manumycin A, manumycin B, colabomycin E, asukamycin A, asukamycin D, β-rubromycin, deoxynybomycin. As comparative substances were used the macrolides antibiotics clarithromycin and azithromycin dehydrate, all of them commercial pharmaceuticals. These substances were tested on the monocytic line THP-1. Cells were stimulated...
|
|
|
The effect of polyketide antibiotics on signalling and functional activity of human monocytic cell line
Kopecká, Kristýna ; Stříž, Ilja (advisor) ; Kovářová, Jaromíra (referee)
Anti-inflammatory cytokines have an important role in the development of inflammatory reactions. If an acute inflammation turns into chronical it is very often a pathological phenomenon. Chronicle inflammations accompany a whole number of serious diseases with an unclear prognosis, such as some of the autoimmune diseases. Usually, the cause of these diseases is not quite clear and the treatment is mainly symptomatic with an effort to suppress the immunity system. For this purpose we use various immunosuppressant drugs, and biological treatment is used, too. Another possibility is to use bioactive secondary metabolites produced by various microorganisms. In this group there are for example macrolides antibiotics, and a big potential is also seen in the recently discovered polyketides. The objective of this work is to test the newly acquired secondary metabolites that were isolated in the Laboratory of Molecular Biology of Actinomycetes at the Czech Academy of Sciences. Tested were manumycin A, manumycin B, colabomycin E, asukamycin A, asukamycin D, β-rubromycin, deoxynybomycin. As comparative substances were used the macrolides antibiotics clarithromycin and azithromycin dehydrate, all of them commercial pharmaceuticals. These substances were tested on the monocytic line THP-1. Cells were stimulated...
|
|
|
The study of the influence of vitamin E analogues on malignant mesothelioma
Kovářová, Jaromíra ; Neužil, Jiří (advisor) ; Kozubík, Alois (referee) ; Vítek, Libor (referee)
Cancer is a leading cause of death in the western world and is increasing in frequency world-wide. Although diagnosis, treatment and therapeutic approaches to cancer have improved, many types of cancer are still lethal due to the lack of radical treatment. One of the fatal neoplastic disease types with poor prognosis is represented by malignant mesothelioma (MM). MM is characterised by very high mortality rate and limited therapeutic options. The etiology of the disease is mainly associated with exposure to asbestos fibres. The incidence of MM is increasing in many countries. The search for novel molecular targets, anti-cancer strategies and drugs, which would considerably improve the treatment is of great importance. Certain new drugs, especially those with specific molecular targets, show high selectivity in their action to cancer cells, and have considerably increased the cure rate in some types of cancer. Mitochondria have recently emerged as a very promising target for anti-cancer agents. A group of compounds with anti-cancer activity that induce apoptosis by way of mitochondrial destabilisation, termed 'mitocans', have been a recent focus of research. Several mitocans have been shown to selectively induce apoptosis in cancer cells and suppress the growth of many types of carcinomas in...
|
guest ::
RSS feed.