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Role of the Erv14 protein in the maintenance of alkali-metal-cation homeostasis in the yeast Saccharomyces cerevisiae
Hrášková, Michaela ; Papoušková, Klára (advisor) ; Vopálenská, Irena (referee)
The Erv14 protein works as a cargo receptor in the COPII vesicles. Many proteins, including Na+, K+/H+ antiporter Nha1, which participates in the maintenance of cell alkali-metal-cation homeostasis, need Erv14 for their trafficking from the ER. When Erv14 is missing, the Nha1 antiporter is partially retained in the ER and its overall transport activity is affected. Although Erv14 interacts with the antiporter through Nha1's transmembrane domains, a shortened version of Nha1 lacking its long hydrophilic C-terminus does not require Erv14 for its efficient trafficking to plasma membrane. This thesis contributes to the understanding of the role of the Erv14 protein in the maintenance of alkali-metal-cation homeostasis in S. cerevisiae cells. Two S. cerevisiae strains lacking ERV14 gene were prepared and the effect of this deletion on the growth and salt tolerance of cells was studied. Using heterologous expression of NHA antiporters from various yeast species with variable lengths of their hydrophilic C-termini we studied localisation and function of these antiporters in S. cerevisiae cells in the presence and in the absence of Erv14 protein. Our results suggested that the length of the antiporter's C-terminus might play a role in its requirement of Erv14 presence for its trafficking through the...
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The role of Erv14 protein in cells of the yeast Saccharomyces cerevisiae
Hrášková, Michaela ; Papoušková, Klára (advisor) ; Macůrková, Marie (referee)
The aim of this bachelor thesis is to summarize all published findings about the role of the Erv14 protein in the cells of Saccharomyces cerevisiae. The Erv14 protein works as a cargo receptor in the endoplasmic reticulum (ER), allowing an incorporation of selected trans-membrane proteins to the COPII vesicles, by which their transport to the Golgi apparatus is allowed. Many proteins were identified in the cells of S. cerevisiae, their transport from the ER depends on the presence of the Erv14 protein. In cells lacking Erv14, these proteins are retained in the ER and are not able to fulfil their functions. One of those proteins is for example Axl2, necessary for axial budding, Sma2, which ensures the standard formation of the prospore membrane during sporulation, the catalytic subunit of the chitin synthase III, Chs3, important for chitin synthesis, and plasma membrane Na+ , K+ /H+ antiporter Nha1 participating in the maintenance of cell alkali-metal-cation homeostasis. The importance of the Erv14 protein's function is proven by the fact that its homologs were also found in other organisms, such as animals, including human, as well as in plants. Keywords: Erv14, cargo receptor, COPII vesicles, transport, Saccharomyces cerevisiae
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