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Study of Biomolecular Interactions with Surface Plasmon Resonance Biosensors
Šípová, Hana ; Homola, Jiří (advisor) ; Houska, Milan (referee) ; Skládal, Petr (referee)
Surface plasmon resonance (SPR) biosensors represent one of the most advanced sensing technologies for real-time studies of biomolecular interactions. In this thesis, methods for functionalization of SPR substrates were optimized and studied via spectroscopic methods. Effects related to the SPR sensor microfluidic interface on the measured biomolecular interactions were analyzed, and furthermore, means to decrease mass-transport limitations were proposed. Several SPR-based assays regarding the detection of nucleic acids were developed, which allow for the detection of physiologically relevant concentrations of nucleic acids as well as point mutations in a nucleic acid sequence. Assays for the determination of the enzymatic activity of HIV integrase and ribonuclease H were developed. These assays can be employed for the design and synthesis of molecules that function either as antiviral drugs or as gene-regulating agents.
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The logic of Charles S. Peirce
Houška, Martin ; Kolman, Vojtěch (advisor) ; Peregrin, Jaroslav (referee)
Tato práce se pokouší zmapovat hlavní přínosy Charlese Sanderse Peirce v logice. Hlavní pozornost zde bude věnována Peircově teorii existenčních grafů, které jsou, mezi ostatními významnými Peircovými objevy, často opomínány. V centrální pasáži o existenčních grafech se pokusíme dostatečně jasně vysvětlit principy fungování těchto grafů a dále ukážeme některé jejich významné vlastnosti. Protože se tato práce věnuje téměř výhradně jen objevům Charlese Sanderse Peirce považuji za vhodné, hned na úvod uvést několik základních informací o jeho životě. Charles Sanders Peirce (18391914), syn Benjamina Peirce (profesora matematiky na Harvardské univerzitě), je světově uznáván jako logik a filosof, ovšem věnoval se i ekonomii, právu a sociálním vědám1. Peirce je ve filosofii znám jako čelní představitel pragmatismu a je mnohými považován za vůbec největšího amerického logika. Množství jeho publikací se pohybuje kolem 12 000 tištěných stran a jeho zatím známé a nevydané manuskripty se počítají na 80 000 ručně popsaných stran. Ač byl Peirce za svého života nedoceňován, jeho práce ovlivnila mnoho generací myslitelů po něm, jmenujme například Schrödera a Peana. Peirce sám stavěl na myšlenkovém odkazu Immanuela Kanta, dále na pracích logiků a matematiků jako byli například Leonhard Euler nebo William z Ockhamu. Charles...
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Simulation of collagen solution flow in rectangular capillary
Kysela, Bohuš ; Skočilas, J. ; Zitny, R. ; Stancl, J. ; Houska, M. ; Landfeld, A.
The viscoelastic properties of foods and polymers can be evaluated from flow of the material in capillary with specified dimension and shape. The extrusion rheometer equipped by capillary with rectangular cross-section was used for determination of the rheological behaviour of water collagen solution. The measurements of the axial profiles in longitudinal direction of the total stresses at capillary wall were performed for various shear rates. The linear viscoelastic model of Oldroyd B type: White-Metzner model was used for simulation of fluid flow in OpenFOAM software package. The simulations describe the effect of relaxation time on wall total stress in convergent-divergent capillary.
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Study of Biomolecular Interactions with Surface Plasmon Resonance Biosensors
Šípová, Hana ; Homola, Jiří (advisor) ; Houska, Milan (referee) ; Skládal, Petr (referee)
Surface plasmon resonance (SPR) biosensors represent one of the most advanced sensing technologies for real-time studies of biomolecular interactions. In this thesis, methods for functionalization of SPR substrates were optimized and studied via spectroscopic methods. Effects related to the SPR sensor microfluidic interface on the measured biomolecular interactions were analyzed, and furthermore, means to decrease mass-transport limitations were proposed. Several SPR-based assays regarding the detection of nucleic acids were developed, which allow for the detection of physiologically relevant concentrations of nucleic acids as well as point mutations in a nucleic acid sequence. Assays for the determination of the enzymatic activity of HIV integrase and ribonuclease H were developed. These assays can be employed for the design and synthesis of molecules that function either as antiviral drugs or as gene-regulating agents.
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Return of Investments and the Impact of Regulations on Poker Market
Lovicar, Adam ; Houška, Milan (advisor) ; Hlavatý, Robert (referee)
This bachelor work deals with analyzing of card game Poker, specifically its version
Texas hold´em no limit. The theoretical part shows basic rules, mathematical and statistical
indicators applied in the poker.This part defines the most important poker terms and concepts
and especially helps to understand the practical part. Firstly the practical part deals with
affiliation regulations and the impact on the economic situation of the player, secondly it
deals with state regulations and the impact on the poker market. Playing field was divided
into two groups, then was detected frequency of games against these groups into two periods.
It was further examinated, if exists a difference between the game against professional and
recrenational players, verified by statistical hypothesis testing. Economical indicator EV and
ROI was used for following part to compare different results of the player PokerPro for each
of the periods. In the last section of the practical part was created a prediction, which
examinated the impact of the changes in the gambling law on the taxes, which should be
payed to the state budget. The prediction was made on particular situation.
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The logic of Charles S. Peirce
Houška, Martin ; Peregrin, Jaroslav (referee) ; Kolman, Vojtěch (advisor)
Tato práce se pokouší zmapovat hlavní přínosy Charlese Sanderse Peirce v logice. Hlavní pozornost zde bude věnována Peircově teorii existenčních grafů, které jsou, mezi ostatními významnými Peircovými objevy, často opomínány. V centrální pasáži o existenčních grafech se pokusíme dostatečně jasně vysvětlit principy fungování těchto grafů a dále ukážeme některé jejich významné vlastnosti. Protože se tato práce věnuje téměř výhradně jen objevům Charlese Sanderse Peirce považuji za vhodné, hned na úvod uvést několik základních informací o jeho životě. Charles Sanders Peirce (18391914), syn Benjamina Peirce (profesora matematiky na Harvardské univerzitě), je světově uznáván jako logik a filosof, ovšem věnoval se i ekonomii, právu a sociálním vědám1. Peirce je ve filosofii znám jako čelní představitel pragmatismu a je mnohými považován za vůbec největšího amerického logika. Množství jeho publikací se pohybuje kolem 12 000 tištěných stran a jeho zatím známé a nevydané manuskripty se počítají na 80 000 ručně popsaných stran. Ač byl Peirce za svého života nedoceňován, jeho práce ovlivnila mnoho generací myslitelů po něm, jmenujme například Schrödera a Peana. Peirce sám stavěl na myšlenkovém odkazu Immanuela Kanta, dále na pracích logiků a matematiků jako byli například Leonhard Euler nebo William z Ockhamu. Charles...
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Application of decision-making models in modernization of weapon equipment of the hunting association Borovy
Mraček, Martin ; Houška, Milan (advisor) ; Martin, Martin (referee)
The aim of this thesis on the application of decision-making models in renewing the arsenal of Borovy Gamekeepers' Organisation (MS Borovy, z.s.) is to select, using a method of multi-criteria decision analysis, a suitable hunting weapon that will best comply with the conditions under which the Borovy Gamekeepers' Organisation (a registered society) exercises its right to hunt and manage game and wildlife. The theoretical part of this paper focuses on weapons, in addition to describing models of multi-criteria decision-making. The second part of the thesis addresses the tradition of hunting and game management as well as various hunting methods, and introduces the Borovy Gamekeepers' Organisation, its hunting grounds and various game species occurring in that area. Subsequently, the author of this paper discusses the selection of a compromise option for a hunting weapon, using a suitable method of multi-criteria decision analysis. Based on the outcome of this selection, a recommendation is made on a suitable hunting weapon for members of the Borovy Gamekeepers' Organisation and the surrounding hunting grounds.
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Routes planning in Úslava microregion
Kůst, Václav ; Houška, Milan (advisor) ; Jana, Jana (referee)
Czech Republic has one of the densest and highest quality marked hiking trails in Europe, covering completely the whole country. But these trails are not planned with respect to the minority of the population with some kind of handicap. This is a group of families with small children who prefer hiking or biking, for families with strollers and disabled persons in wheelchairs. As the author of this thesis is a councilor of the village Jarov and also a councilor of this village in the Mikroregion Úslava organization he decided to plan a short circular hiking trails with respect to these neglected tourist groups in order to expand tourism in the area of Mikroregion Úslava. These routes may also partly overlap with marked hiking trails (due to their density) and are planned with practical experiences of the author. The results of the thesis will be incorporated into the information brochures of Mikroregion Úslava.
The thesis is divided into theoretical and practical part. Theoretical part describes the methods for solving the travelling salesman problem and provides general information on the concept of micro-regions - voluntary unions of the municipalities. The practical part describes Mikroregion Úslava, the points of interest database creation procedure and planning sightseeing routes for families with children on foot, by bicycle or in strollers. There is also planned a route for disabled persons in wheelchairs in Blovice, which the author of the thesis eventually eliminated from the reasons described.
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