|
|
Comparison of in vitro methods for the study of cytotoxicity
Eliášová, Pavla ; Maixnerová, Jana (advisor) ; Bárta, Pavel (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Pavla Eliášová Supervisor: RNDr. Jana Maixnerová, Ph.D. Title of diploma thesis: Comparison of in vitro methods for the study of cytotoxicity People are exposed to a growing number of toxic substances from the environment. Endocrine disrupting chemicals (EDCs) are a broad category of molecules that are thought to cause adverse effects on the endocrine system by interfering with the synthesis, transport, degradation or action of endogenous ligands. One of the aims of this thesis was to determine the in vitro toxicity of 17 selected endocrine disruptors on the human hepatocellular carcinoma HepG2 cell line. Cell viability was determined using the CellTiter96® AQueous One Solution Cell Proliferation Assay colorimetric method, the principle of which is the reduction of MTS to the colored product formazan by mitochondria in viable cells. The cytotoxic potential of the compounds was expressed by using the toxicological parameter IC50, which was measured in three time intervals (6, 12 and 24 hours). For 14 substances: Atrazine, DHEP, Bisphenol A, Carbofuran, 3-hydroxycarbofuran, Cypermethrin, DDE, DES, MEHP, PCB 118, PCB 153, PFOA, PFOS, Propiconazole, IC50 > 100 µM (respectively > 250 µM) was...
|
|
|
Preparation of hierarchical zeolites for fine chemical synthesis
Veselý, Ondřej ; Eliášová, Pavla (advisor) ; Přech, Jan (referee)
Preparation of hierarchical zeolites for fine chemical synthesis Author: Bc. Ondřej Veselý Supervisor: Mgr. Pavla Eliášová, Ph.D. Prague, 2019 Abstract (in english): The thesis is focused on synthesis of hierarchical (micro-mesoporous) zeolites by several different methods and their application in catalytic reactions. Performance of hierarchical materials prepared by different approaches has been investigated, as well as the effect of framework topology and type of acidity on the outcome of the reactions. The work was elaborated in the Department of Physical and Macromolecular Chemistry of Charles University under the supervision of Mgr. Pavla Eliášová, Ph. D. The work is divided into three parts. In the first part several methods of preparation of hierarchical zeolites have been investigated and compared in catalytic reactions. Desilication, selective removal of silicon from the framework, is a post-synthetic method that can be used to introduce additional mesoporosity into a zeolite. The process leads to formation of mesopores by introducing defects into the zeolite structure. The resulting mesopore size is very broad. To partially control the pore size, alkylammonium cations may be added to the solution to protect the crystal surface. The desilication was performed on MTW zeolite which contains...
|
|
|
Preparation of hierarchical zeolites for fine chemical synthesis
Veselý, Ondřej ; Eliášová, Pavla (advisor) ; Přech, Jan (referee)
Preparation of hierarchical zeolites for fine chemical synthesis Author: Bc. Ondřej Veselý Supervisor: Mgr. Pavla Eliášová, Ph.D. Prague, 2019 Abstract (in english): The thesis is focused on synthesis of hierarchical (micro-mesoporous) zeolites by several different methods and their application in catalytic reactions. Performance of hierarchical materials prepared by different approaches has been investigated, as well as the effect of framework topology and type of acidity on the outcome of the reactions. The work was elaborated in the Department of Physical and Macromolecular Chemistry of Charles University under the supervision of Mgr. Pavla Eliášová, Ph. D. The work is divided into three parts. In the first part several methods of preparation of hierarchical zeolites have been investigated and compared in catalytic reactions. Desilication, selective removal of silicon from the framework, is a post-synthetic method that can be used to introduce additional mesoporosity into a zeolite. The process leads to formation of mesopores by introducing defects into the zeolite structure. The resulting mesopore size is very broad. To partially control the pore size, alkylammonium cations may be added to the solution to protect the crystal surface. The desilication was performed on MTW zeolite which contains...
|
|
| |
|
|
Synthesis, characterization and catalytic application of novel zeolites
Eliášová, Pavla ; Čejka, Jiří (advisor) ; Štěpnička, Petr (referee) ; Mintova, Svetlana (referee)
The PhD thesis concerns the synthesis of novel zeolite materials, investigation of their properties and their possible use in catalytic application. The work was focused on the two- dimensional zeolites. The thesis was worked out at the Department of Synthesis and Catalysis at J. Heyrovský Institute of Physical Chemistry, AS CR. Germanosilicate UTL (Si/Ge molar ratio 4.0-6.5) was found to undergo unique structural changes in the neutral or acid environment leading to transformation of its three-dimensional framework into two-dimensional layered material denoted IPC-1P. The UTL degradation, so called top-down synthesis, was enabled due to a presence of double-four-units (D4Rs), which can be seen as supporting units/pillars between the rigid layers. The preferential location of Ge in D4Rs makes the units an ideal target for their selective degradation. The interlayer space in lamellar IPC-1P was modified by swelling with long-organic chain surfactant (material IPC-1SW). To keep the interlayer space permanently expanded (up to 3.3 nm) the silica amorphous pillars were subsequently introduced (material IPC-1PI). The integrity of the layers and their preserved UTL character was confirmed in all members of IPC-1 family by HRTEM and electron diffraction measuring. The layers of IPC-1P were condensed back...
|
guest ::
RSS feed.