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National Repository of Grey Literature

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	Optimalization of nested-PCR for the detection of candidal DNA in biological samples. Kvasničková, Erika ; Buchta, Vladimír (advisor) ; Bolehovská, Radka (referee) Detailed record
	Possibilities of UCP2 (uncoupling protein 2) induction in rat hepatocytes in in vivo conditions Bolehovská, Radka ; Červinková, Zuzana (advisor) ; Kalous, Martin (referee) ; Novotný, Dalibor (referee) Possibilities of UCP2 (uncoupling protein 2) induction in rat hepatocytes in in vivo conditions Introduction Uncoupling protein 2, discovered in 1997, is the first described homologue of uncoupling protein 1. Uncoupling proteins increase the permeability of inner mitochondrial membrane for protons, decrease the efficiency of energy conversion, inhibit the ATP synthesis and stimulate energy release in form of heat. Uncoupling proteins also increase the substrate oxidation and reduce production of reactive oxygen species in mitochondria. Objective, material and method The aim of this study was to establish and optimised the quntitative real-time PCR for detection of UCP2 mRNA expression kinetics in rat liver tissue. The present study was conducted to assess the effects of acute and chronic treatment with triiodothyronine and the effect of partial hepatectomy on liver uncoupling protein 2 mRNA levels in male Wistar rats. Results Intraperitoneal injection of one dose of triiodothyronine (200 μg/kg rat body weight) increased mRNA expression of uncoupling protein 2 in liver tissue almost 2-fold (P < 0.01 vs. control group) in rats 12 hours after T3 administration. Concentrations of total triiodothyronine and free triiodothyronine in serum were increased 122-fold and 77-fold (p < 0.001), respectively.... Detailed record
	Optimalization of nested-PCR for the detection of candidal DNA in biological samples. Kvasničková, Erika ; Buchta, Vladimír (advisor) ; Bolehovská, Radka (referee) Detailed record

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