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Determination of hyaluronan by electromigration methods
Bártová, Adéla ; Mravcová, Ludmila (referee) ; Řezáčová, Veronika (advisor)
This thesis is focused on description hyaluronic acid, characterization of its behaviour in water solutions, its chemical-physical properties and its using in different industrial sectors. The next part deals with possibilities of its analysis. The following section of the thesis is focused on electromigration techniques with special attention dedicated to capillary electrophoretic methods, especially capillary zone electrophoresis. The aim of this thesis is optimization of the method of determination of hyaluronic acid in water solutions by capillary zone electrophoresis.
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Analysis of volatile organic compounds produced by monocytes during sepsis
Bártová, Adéla ; Řezáčová, Veronika (referee) ; Mravcová, Ludmila (advisor)
This thesis is focused on the possibility of analysis of volatile organic compounds produced by monocytes during sepsis. Method of comprehensive two-dimensional gas chromatography with mass spectrometric detection was chosen for this purpose. Content of the first part was the optimization of the method of two-dimensional gas chromatography for the determination of volatile organic compounds. In this part were gradually adjusted parameters of the gas chromatography method to achieve the maximum efficiency. Further were adjusted conditions of samples preparation. Content of the second part was the usage of already optimized method for the analysis of the samples set of monocytes. Samples were subjected to the action of different inhibitors of the immune system and stimulators simulating bacterial or yeast infection. Based on this analysis were identified some compounds, which are produced by monocytes under condition simulating the infection.
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Psychic burden of a kindergarten teacher during the SARS-CoV-2 coronavirus pandemic
BÁRTOVÁ, Adéla
The bachelor's thesis focuses on the issue of psychological stress in job of kindergarten teachers. More specifically, it discusses the burden during the SARS-CoV-2 coronavirus pandemic. The aim of the work is to ascertain how the SARS-CoV-2 pandemic affected the interviewed kindergarten teachers.The theoretical part presents the topic of stress and burden in connection with the profession of kindergarten teacher. The thesis is also aimed to burnout syndrome and its prevention. The empirical part centeres to the analysis of data obtained through qualitative research. The questions of the semistructured interview with the five kindergarten teachers concerned mainly the experience of the situation, the changes due to the pandemic and the burnout syndrome. Final results showed that the situation was more difficult for kindergarten teachers, paradoxically they did not show any symptoms of increased mental stress.
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Enzymatic oxidations of alcohols in biosynthesis of bumblebee pheromones
Bártová, Adéla ; Valterová, Irena (advisor) ; Bosáková, Zuzana (referee)
Secretion of cephalic labial gland of Buff-tailed bumblebee males (Bombus terrestris) contains a mixture of terpene alcohols, aliphatic alcohols, esters and alkanes with small amount of aldehydes potentially biosynthetized of (S)-2,3-dihydrofarnesol and geranylcitronellol (major alcoholic compounds). This secretion acts as a marking and luring pheromone during patrolling. This study is focused on oxidation of terpene alcohols using enzymes of cephalic labial gland of a bumblebee. In vitro incubations were accomplished with homogenate of cephalic labial gland and a various substrates: E,E-farnesol, geraniol, 2,3-dihydrofarnesol, hexadecanol. Conditions of incubations and following analysis using gas chromatography were optimized. Two products were detected of farnesol and geraniol incubations, which then later were identified using chemical oxidation with pyridinium-chlorochromate, measurement of NMR specters and MS specters, as two isomers of 2,3-bond. Hexadecanol was not oxidized.
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Influencers and their perceptions of authenticity and credibility on social media
Bártová, Adéla ; Vochocová, Lenka (advisor) ; Reifová, Irena (referee)
This diploma thesis is devoted to the phenomenon of lifestyle influencers and the factors of authenticity and credibility. The work aims to bring the view of the community itself on the issue of the already mentioned factors in the field of social media and at the same time to find out whether there are specific strategies for their achievement. In addition, the work also aims to explore the role of authenticity and credibility for commercial entities, specifically for marketing experts. In the introduction, I explain the concept of influencers, the associated influencer marketing, and what factors can affect the success of influencers. In the second chapter, I focus on social media, how their arrival has affected the media sphere, and clarify the positives and negatives that are associated with their existence. I also pay attention to authenticity and credibility, two fundamental factors for this thesis. I explain their meaning, I present the indicators that have been defined so far for their detection and I also approach the specific strategies that are used by influencers. The practical part of the work is based on a qualitative method of semi-structured interviews with six selected lifestyle influencers and four marketing experts. Data analysis provides insight into the environment of influencers, from...
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Analysis of volatile organic compounds produced by monocytes during sepsis
Bártová, Adéla ; Řezáčová, Veronika (referee) ; Mravcová, Ludmila (advisor)
This thesis is focused on the possibility of analysis of volatile organic compounds produced by monocytes during sepsis. Method of comprehensive two-dimensional gas chromatography with mass spectrometric detection was chosen for this purpose. Content of the first part was the optimization of the method of two-dimensional gas chromatography for the determination of volatile organic compounds. In this part were gradually adjusted parameters of the gas chromatography method to achieve the maximum efficiency. Further were adjusted conditions of samples preparation. Content of the second part was the usage of already optimized method for the analysis of the samples set of monocytes. Samples were subjected to the action of different inhibitors of the immune system and stimulators simulating bacterial or yeast infection. Based on this analysis were identified some compounds, which are produced by monocytes under condition simulating the infection.
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Biosynthesis of the bumblebee male pheromones and its hormonal regulation.
Bártová, Adéla ; Valterová, Irena (advisor) ; Smrček, Stanislav (referee)
Bumblebees are important pollinators, commercially used in large-scale plant growing in greenhouses. Their males produce marking pheromones for mating, which attract young bumblebee queens. These pheromones are often a complicated mixture of chemicals, which is produced in the male labial gland, and the mixture itself is specific for each bumblebee species. The regulation of bumblebee sexual pheromone biosynthesis is largely unknown, and this Master's thesis is focused on the analysis of the mechanisms which lead to the regulation of the Bombus terrestris male pheromone's fat-acid and terpenes biosynthesis, specifically on stereospecific enzymatic reduction of double bond of farnesol. This thesis studies the influence of potential neurohormones on a specific enzymatic mixture, which is involved in the pheromone biosynthesis. Methods used in this project include biochemical, analytic and molecular-biology methods.
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Enzymatic oxidations of alcohols in biosynthesis of bumblebee pheromones
Bártová, Adéla ; Valterová, Irena (advisor) ; Bosáková, Zuzana (referee)
Secretion of cephalic labial gland of Buff-tailed bumblebee males (Bombus terrestris) contains a mixture of terpene alcohols, aliphatic alcohols, esters and alkanes with small amount of aldehydes potentially biosynthetized of (S)-2,3-dihydrofarnesol and geranylcitronellol (major alcoholic compounds). This secretion acts as a marking and luring pheromone during patrolling. This study is focused on oxidation of terpene alcohols using enzymes of cephalic labial gland of a bumblebee. In vitro incubations were accomplished with homogenate of cephalic labial gland and a various substrates: E,E-farnesol, geraniol, 2,3-dihydrofarnesol, hexadecanol. Conditions of incubations and following analysis using gas chromatography were optimized. Two products were detected of farnesol and geraniol incubations, which then later were identified using chemical oxidation with pyridinium-chlorochromate, measurement of NMR specters and MS specters, as two isomers of 2,3-bond. Hexadecanol was not oxidized.
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Determination of hyaluronan by electromigration methods
Bártová, Adéla ; Mravcová, Ludmila (referee) ; Řezáčová, Veronika (advisor)
This thesis is focused on description hyaluronic acid, characterization of its behaviour in water solutions, its chemical-physical properties and its using in different industrial sectors. The next part deals with possibilities of its analysis. The following section of the thesis is focused on electromigration techniques with special attention dedicated to capillary electrophoretic methods, especially capillary zone electrophoresis. The aim of this thesis is optimization of the method of determination of hyaluronic acid in water solutions by capillary zone electrophoresis.
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Case study of physiotherapy treatment of a patient with the diagnosis of Multiple Sclerosis
BÁRTOVÁ, Adéla
The thema of this bachelor thesis is "Case study of physiotherapy treatment of a patient with the diagnosis of Multiple Sclerosis". The severity of multiple sclerosis incrementaly increases as the incidency of this disease - globaly and in the Czech Republic as well - rapidly grows year by year. Multiple sclerosis -as an autoaggressive chronic disease - is still incurable, however, plenty of changes in the diagnosis and the therapy have emerged during last years. This illness afflicts mostly persons during the productive period of their lifes. The patient is often without clinical symptoms during the first few years, which, until recently, misled to an idea that there is no urgent need to apply a long-term medical treatment. Today it is evident that only early executed diagnosis and early started therapy afterwards can prevent from the permanent neurologic impairment of the patient. Besides the pharmacological treatment, there is also physiotherapy which helps patients to live an active and valuable life. The point of this paper is to illustrate a complex view of this problem. The first, theoretical part deals with multiple sclerosis as a whole, with the goal to summarize the latest knowledge and findings about this disease. It describes the potential causes of the origin of the disease, its occurrence, particular symptoms, development of the disease, different types of the disease and the possible diagnosis together with the differential diagnosis. The ending of the theoretical part informs about the therapy comprising the pharmacological treatment and the complications it may bear, and the rehabilitation treatment summarizing therapy of all symptoms, which can appear. Nowadays, it is possible to find different approaches in the rehabilitation of multiple sclerosis. But according to many physiotherapy studies those methodologies, which are based on the neurophysiological fundamentals, were already very positively aplied on the patients. It is an objective of the practical part of my thesis to introduce one of these methodologies. The impact of an aplication of the individual physiotherapy, which utilizes the neurophysiological findings, can be evaluated by comparing the results from the entering and exit. This work is conceived as a case study in the form of casuistry. Qualitative research based on the data collection was chosen to verify the efficiency of the aplied physiotherapy. Data was obtained by interviews, observation, analyses of medical records and kinesiology testing. An entering and exit examination, comprised from the set of tests, were performed after the six months therapy to assess changes in the clinical conditions of the patient. These tests allows the therapist to comprehensively evaluate the effect of the rehabilitation on the patients with the central disabilities, particularly on the patients with multiple sclerosis. Tests were selected regarding the clinical signs of the disease. There were examinations done to test the balance, dysmetria, dysdiadochokinesia, scales of postural reactions, walking, muscle strength and spasticity. The test of grabbing ability was done to examinate the light motor skills and goniometry was aplied to determine the joint's range of mobility. The results of the research proven, that the individual physiotherapy employing findings in neurophysiology together with aerobic therapy and other techniques appropriately selected with the respect to the actual complications of the patient has a positive effect on the overall condition of the patient. This bachelor thesis can serve as a theoretical basis for the teaching needs or as an informational source for all, who are interested in the multiple sclerosis disease issue. It can also be an inspiration for physiotherapists on how to proceed in the rehabilitation of patients with multiple sclerosis.
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