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Biotechnological production of sophorolipids
Šimšová, Veronika ; Sedláček, Petr (referee) ; Obruča, Stanislav (advisor)
This diploma thesis deals with the microbial production of sophorolipids by the Starmerella genus yeasts. The theoretical part of the thesis includes general characteristics of biosurfactants with the focus on sophorolipids. There are described the options of biotechnological production of sophorolipids and fields of their possible applications. Furthermore, the theoretical part deals with the describtion of Starmerella bombicola yeast, which is often used for biotechnological production of sophorolipids. Six strains of the Starmerella genus were cultured in the basic medium suitable for sophorolipid produsction. The amount of produced sophorolipids was tested by the emulsification capacity assay, solubilization of crystalline anthracene assay, measuring the surface tension by the Du-Noüy-Ring method and determination of the sophorolipid concentration by extraction with ethyl acetate. The purity of the extracted sophorolipids was inspected by the Fourier Transform infrared spectrosopy (FT-IR) Based on the results, the Starmerella bombicola CBS 6009 and the Starmerella anomalae CBS 14178 strains were studied in greater detail. They were cultured in several production media of different composition and the effect of the individual components on the ability of the sophorolipid production was monitored. Based on the results, it was evaluated that the composition of the medium has a great influence on the production ability of the strains. The highest production rate of sophorolipids was achieved in the basic production medium and so was in the medium containing molasses and Indian waste oil. The cultivation mode has great effect on the sophotolipid production rate. It has been found that when cultured in a bioreactor, the strains produced sophorolipids in a larger amount and of a different quality than in the shaker flasks.
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Bioactive substances contained in elderberry pomace
Šimšová, Veronika ; Diviš, Pavel (referee) ; Vespalcová, Milena (advisor)
This bachelor thesis deals with the determination of selected chemical characteristics in the elderberry juice, compote and in the extracts of elderberry marc. The theoretical part describes the botanical characteristics of elderberry (Sambucus nigra L.) including its occurrence, the possibility of utilization of individual parts of the plant in the food industry and in the healing therapies and chemical composition of the berries. The biggest attention is given to the bioactive compounds contained in these fruits. The second chapter deals with the dry matter and the methods of its determination. The last chapter of the theoretical part describes vitamin C, its importance for the human organism and manual methods for determination of vitamin C. Among the selected chemical characteristics, set out in the experimental part, was the determination of reducing sugars by gravimetric method and Berthrand method, titratable acids, total anthocyanins by pH-differential method, total phenolic compounds by the Folin-Ciocalteu method and determination of the dry matter by drying and refractometric method. Analyzes have proven that extracts made of elderberry marc under suitable conditions have, in most cases, chemical characteristics comparable to the elderberry juice and compote. However, extracts are much better source of anthocyanic and phenolic compounds than juice and compote.
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Biotechnological production of sophorolipids
Šimšová, Veronika ; Sedláček, Petr (referee) ; Obruča, Stanislav (advisor)
This diploma thesis deals with the microbial production of sophorolipids by the Starmerella genus yeasts. The theoretical part of the thesis includes general characteristics of biosurfactants with the focus on sophorolipids. There are described the options of biotechnological production of sophorolipids and fields of their possible applications. Furthermore, the theoretical part deals with the describtion of Starmerella bombicola yeast, which is often used for biotechnological production of sophorolipids. Six strains of the Starmerella genus were cultured in the basic medium suitable for sophorolipid produsction. The amount of produced sophorolipids was tested by the emulsification capacity assay, solubilization of crystalline anthracene assay, measuring the surface tension by the Du-Noüy-Ring method and determination of the sophorolipid concentration by extraction with ethyl acetate. The purity of the extracted sophorolipids was inspected by the Fourier Transform infrared spectrosopy (FT-IR) Based on the results, the Starmerella bombicola CBS 6009 and the Starmerella anomalae CBS 14178 strains were studied in greater detail. They were cultured in several production media of different composition and the effect of the individual components on the ability of the sophorolipid production was monitored. Based on the results, it was evaluated that the composition of the medium has a great influence on the production ability of the strains. The highest production rate of sophorolipids was achieved in the basic production medium and so was in the medium containing molasses and Indian waste oil. The cultivation mode has great effect on the sophotolipid production rate. It has been found that when cultured in a bioreactor, the strains produced sophorolipids in a larger amount and of a different quality than in the shaker flasks.
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Bioactive substances contained in elderberry pomace
Šimšová, Veronika ; Diviš, Pavel (referee) ; Vespalcová, Milena (advisor)
This bachelor thesis deals with the determination of selected chemical characteristics in the elderberry juice, compote and in the extracts of elderberry marc. The theoretical part describes the botanical characteristics of elderberry (Sambucus nigra L.) including its occurrence, the possibility of utilization of individual parts of the plant in the food industry and in the healing therapies and chemical composition of the berries. The biggest attention is given to the bioactive compounds contained in these fruits. The second chapter deals with the dry matter and the methods of its determination. The last chapter of the theoretical part describes vitamin C, its importance for the human organism and manual methods for determination of vitamin C. Among the selected chemical characteristics, set out in the experimental part, was the determination of reducing sugars by gravimetric method and Berthrand method, titratable acids, total anthocyanins by pH-differential method, total phenolic compounds by the Folin-Ciocalteu method and determination of the dry matter by drying and refractometric method. Analyzes have proven that extracts made of elderberry marc under suitable conditions have, in most cases, chemical characteristics comparable to the elderberry juice and compote. However, extracts are much better source of anthocyanic and phenolic compounds than juice and compote.
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