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Synthesis of Lead and Lead Oxide Nanoparticles for Inhalation Experiments
Moravec, Pavel ; Smolík, Jiří ; Ondráček, Jakub ; Vodička, Petr ; Fajgar, Radek
From the three tested methods of NP's generation the evaporation/condensation of metallic Pb seems to be the most suitable for long lasting inhalation experiments due to its simplicity, unambiguous mechanism of NP′s formation and well defined composition. Nevertheless, NP′s can change their composition, at least on the surface layer, during the time-lag between their formation and inhalation. Thermal decomposition of PbTHD2 can produce sufficiently high concentration of NP′s but there is some uncertainty about their composition due to presence of OC in NP′s. Oxidation of PbTHD2 produces carbon free NP′s, but the number concentration of NP′s is somewhat low.
Plný tet: SKMBT_C22013101814595 -  PDF
Plný text: content.csg - PDF
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Generation of Pb/PbOx Nanoparticles for Inhalation Experiments
Moravec, Pavel ; Smolík, Jiří ; Ondráček, Jakub ; Vodička, Petr ; Fajgar, Radek
Health aspects of nanoparticles (NP′s) on living beings are considered to be important. However, the real data of the impact of NP′s inhalation are rather rare. A study of allocation of MnOx nanoparticles in organs of laboratory animals in inhalation chamber was presented by Večeřa and Mikuška (2012). For these experiments a source of continual generation of nanoparticles in duration of days or even weeks and in appropriate concentration is necessary. In this work a study of continual generation of Pb/PbOx nanoparticles in hot wall tube reactor are presented.
Plný tet: SKMBT_C22013091912130 - PDF
Plný text: content.csg - PDF
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Inhalace nanočástic MnxOy
Mikuška, Pavel ; Večeřa, Zbyněk ; Dočekal, Bohumil
Příspěvek se zabývá inhalací nanočástic MnxOy malými experimentálními zvířaty a studiem alokace Mn v orgánech exponovaných myší. Obsah Mn v orgánech exponovaných myší byl porovnán s obsahem Mn v orgánech kontrolního vzorku myší, které inhalaci nanočásticemi MnxOy nebyly podrobeny.
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Unique exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk ; Mikuška, Pavel ; Moravec, Pavel ; Smolík, Jiří
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs“. The inhalation chamber builds from a glass and stainless steel and makes possible long-term inhalation procedures (up to 60 days, 24 hours/day) with up to four discrete groups of mice population (up to 60 individuals each) or of rats population (up to 20 individuals each) under controlled illumination, temperature, relative humidity, doses (0.5 x 106 - 5 x 106 particles/cm3) and size (10-60 nm) of nano-sized particles which are synthesized via aerosol route in a hot wall tube flow reactor using metal organic precursors.
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Characterization of atmospheric particulate matter by diffusive gradient in thin film (DGT) technique
Gregušová, Michaela ; Dočekal, Bohumil
In this study, characteristics of heavy metal contamination in road dust collected from urban area with heavy traffic in Brno, Czech Republic was investigated. The DGT probes were exposed in aqueous suspensions of particulate matter for different time periods to monitor responses of the sample matter. The road dust and DGT sorption gels were decomposed by microwave assisted extraction and analyzed by inductively coupled plasma mass spectrometry for estimation of trace metals contents. The results revealed that the traffic appears to be responsible for the high levels of Cu, Pb, Ni and Sb and also that DGT technique, as alternative to conventional extraction procedures is a useful tool for characterization of metals mobility.
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První zkušenosti s inhalací nanočástic malými zvířaty
Mikuška, Pavel ; Večeřa, Zbyněk
Příspěvek popisuje inhalační komoru konstruovanou pro dlouhodobé inhalační studie malých zvířat nanočásticemi za přísně kontrolovaných podmínek (tj., teplota, relativní vlhkost, světlo, tlak). Nanočástice byly syntetizovány on-line v trubkovém průtokovém reaktoru z kovových organických prekursorů. Experimenty potvrdily, že inhalační komora je vhodná pro „whole body“ experimenty s malými zvířaty.
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