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Influence of the O-phosphorylation of serine, threonine and tyrosine in proteins on the amidic N-15 chemical shielding anisotropy tensors
Emmer, J. ; Vavrinská, A. ; Sychrovský, Vladimír ; Benda, Ladislav ; Kříž, Z. ; Koča, J. ; Boelens, R. ; Sklenář, V. ; Trantírek, L.
Density functional theory was employed to study the influence of O-phosphorylation of serine, threonine, and tyrosine on the amidic N-15 chemical shielding anisotropy (CSA) tensor in the context of the complex chemical environments of protein structures.
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Sensor based on Förster resonance energy transfer with quantum dot
Lišková, Marcela ; Datinská, Vladimíra ; Klepárník, Karel ; Foret, František
Förster resonance energy transfers (FRET) are based on the nonradiative transfer of energy between a donor and acceptor dye in the distance 1-10 nm. With respect to this, quantum dots (QDs) with a high absorbance can serve as suitable donors of energy in FRET. Here, we present architectures of two sensors based on QD conjugates useful in imaging and diagnostics of cancer. One sensor, based on an oligonucleotide conjugate, can be used for the detection of complementary oligonucletide chains. The other was designed for the detection of enzymes inside cells.
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Characterization of atmospheric particulate matter by diffusive gradient in thin film (DGT) technique
Gregušová, Michaela ; Dočekal, Bohumil
In this study, characteristics of heavy metal contamination in road dust collected from urban area with heavy traffic in Brno, Czech Republic was investigated. The DGT probes were exposed in aqueous suspensions of particulate matter for different time periods to monitor responses of the sample matter. The road dust and DGT sorption gels were decomposed by microwave assisted extraction and analyzed by inductively coupled plasma mass spectrometry for estimation of trace metals contents. The results revealed that the traffic appears to be responsible for the high levels of Cu, Pb, Ni and Sb and also that DGT technique, as alternative to conventional extraction procedures is a useful tool for characterization of metals mobility.
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