National Repository of Grey Literature 13 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
The platelet aggreagability induced by low concentrations of ADP and epinephrine in patients with breast cancer.
Razimová, Jana ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
Background: Thrombosis is an important cause of morbidity and mortality in cancer patients. There are many blank spaces in our knowledge on the pathogenesis of cancer associated thrombosis. The sticky platelet syndrome is an inherited disorder characterized by platelet hyperaggregability with low concentrations of ADP and/or epinephrine. The platelet aggregation with low concentrations of ADP and epinephrine has not yet been investigated in breast cancer patients treated with endocrine therapy. Aims of the study: Primary objective: To analyze the platelet aggregation with low concentrations of ADP and epinephrine in patients with breast cancer treated with endocrine therapy using light transmission aggregometry and compare the results with the results of the platelet aggregation in healthy volunteers. To compare the proportion of patients fulfilling the laboratory criteria for sticky platelet syndrome with the proportion of healthy volunteers fulfilling these criteria. Secondary objectives: 1. To analyze the correlation of aggregation with low concentrations of inductors with the expression of P-selectin on the non stimulated platelets in the group of breast cancer patients treated with endocrine therapy and in the group of healthy volunteers. 2. To compare the expression of P-selectin on the non...
Quality Control in Haematology laboratory
Hadysová, Zuzana ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
Background: In the theoretical part there was my task to carry out a search of internal and external quality control in hematology laboratory. In the experimental part of the work I focused on the description of quality control of laboratory IV. Internal Hematology Clinic of the University Hospital Hradec Kralove. Main findings: Many prevention and control activities serving to detect any errors and failures and to evaluate quality control system are part of quality control in hematology laboratory. Every laboratory (small or large) has developed principles for quality control which must be followed. Conclusions: Quality control is important part of every laboratory, it helps to identify and eliminate analytical and non-analytical errors in the process of obtaining correct laboratory results. It is necessary to constantly work on the improvement because it increases the confidence of patients, medical staff and also laboratory workers in the accuracy of the results issued by the laboratory. Keywords: internal quality control, external quality control, hematology laboratory
Analysis of body fluids on the automatic analyzer XT - 4000i
Tycová, Blanka ; Šatínský, Dalibor (advisor) ; Sadílek, Petr (referee)
ABSRACT Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Blanka Tycová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of rigorous thesis: Analysis of body fluids on the automatic analyzer XT - 4000i This rigorous thesis focuses on the cytologic analysis of body fluids, which are analyzed in hematology laboratory OKH in the Pardubice regional hospital, a.s. These are the cerebrospinal fluid, pleural, pericardial and abdominal fluid, peritoneal dialysate and synovial fluid. From the measured data during the analysis of body fluids has been to focus on quality control and determine precision, truthfulness, linearity measurements, and the stability of peritoneal dialysate in the laboratory (21.7 řC) and cooler temperatures (6.9 řC). Statistical evaluation of the measured data showed that the analyzer provides the valid values. Graphic processing has been shown, that the measurement actually conducted linearly. The experiment confirmed, that greater stability shows a sample of peritoneal dialysate, that has been kept at a cooler temperature (6.9 řC). In this rigorous thesis were performed correlation analysis results of analysis the body fluids in the automatic analyzer XT - 4000i and microscopic method, which used a Fuchs - Rosenthal counting...
Possibilities of Laboratory Diagnostics of Lupus Anticoagulant Antibodies in the Special Hematology Laboratory of the Institute for Clinical and Experimental Medicine in Prague
Rybníčková, Soňa ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
Rybníčková Soňa THE POSSIBILITY OF ASSESSMENT OF LUPUS ANTICOAGULANT ANTIBODIES IN HEMATOLOGY LABORATORY IKEM IN PRAGUE Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Laboratory Technician Background Diagnosis of the Lupus Anticoagulant is very complicated due to a missing completely reliable screening test and whenever the presence of prolonged clotting times on a routine plasma test is detected, the other potential causes besides LA is needed to exclude. The aim of the thesis is focused on a possibilities of testing the Lupus Anticoagulant in our Clinical Haematology Department in the Institute for Clinical and Experimental Medicine inclusive of ambiguity measured results interpretation and a necessity to abide by the conditions of a preanalytic phase. In addition, clinical manifestation and laboratory criteria assessment as well as a possible interferences in Lupus Anticoagulant testing are also discussed. Methods The theoretical part is focused on a basic mechanism of haemostasis, on an effect of antiphospholipid antibodies, the diagnostic kriteria inclusive of a treatment guidelines. The practical part is focused on importance of the preanalytical phase, principally on necessity of a double centrifugation tube method (to obtain platelet pure plasma). More over is...
Comparison of basic coagulation methods - APTT, PT, FBG by mechanical and optical principle
Hricová, Júlia ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
In my thesis, the basic coagulation methods APTT, PT and fibrinogen were compared with the use of an optical and impedance principle. The coagulation analyser Sysmex CA 7000 was used for measuring coagulation tests using an optical method and the coagulation analyser AMAX Destiny Plus measured the formation of clots using an impedance method. In a theoretical part of my thesis, I described the process of formation of clots as well as the detection principles of the two analysers mentioned. I mainly focused on possible interferences which can influence the values of coagulation tests. I described how these interferences could develop, the mechanism of their influence on measured parameters and how their effects could be removed. In a practical part of my thesis, I described a preanalytic part of blood taking, the coagulation analysers and a principle of their measurement, an internal quality control and the reference values for these tests. For the coagulation tests APTT, PT and fibrinogen were used patient's normal, haemolytic, lipemic and icteric plasma. T-test done in Microsoft Excel was used to assess similarities or differences between the optical and measured impedance results. To see the differences between methods, graphs of mean values of each method were created. For the conclusion of my...
Implementation of Antiplatelet Therapy Monitoring on the Analyzer Multiplate
Trenčanská, Šárka ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
This study describes the implementation of antiplatelet therapy monitoring on the analyzer Multiplate that works on the principle of impedance aggregometry. We used to determine four tests - ASPItest used for monitoring acetyl salicylic acid (ASA) therapy, ADPtest and ADPtestHS used to monitoring thienopyridines treatment and TRAPtest , which is used to monitoring treatment with GPIIb/IIIa inhibitors. The aim of this study was to verify the accuracy of this method for individual tests, to determine its own reference limits and compare them with literature data and on a group of cardiac patients receiving dual antiplatelet therapy (aspirin 100 mg per day and clopidogrel 75 mg per day) to assess any resistance to these drugs. Accuracy of the method we verified the group of 10 blood donors without any treatment. Each sample was repeatedly measured 5 times and from the measured values we calculated coefficients of variation. Our values of coefficients of variation from the values given by the manufacturer (CV 6 - 10 %) did not differ significantly (ASPItest: CV 8.7 %, ADPtest: CV 8.8 %, ADPtestHS: CV 13.3 % and TRAPtest: CV 12.1 %). We also verified on a group of 32 blood donors without any treatment reference ranges AUC for single tests, which were compared with reference limits from the literature....
Modern Trends in Sample Preparation of Biological Material for Analysis of Selected Biologically Active Compounds
Sadílek, Petr ; Solich, Petr (advisor) ; Nobilis, Milan (referee) ; Barták, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Petr Sadílek, M.Sc. Supervisor: Prof. RNDr. Petr Solich, CSc. Co-supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Doctoral Thesis: Modern Trends in Sample Preparation of Biological Material for Analysis of Selected Biologically Active Compounds This doctoral thesis deals with the determination of selected drugs, their metabolites and other biologically active substances in biological materials. This area of instrumental analysis turns out to be very attractive recently. The reason for this is the need to monitor and optimise treatment and the goal is to achieve the most effective therapy with minimum side effects of drugs for every individual; there are also toxicological reasons. The thesis concentrates on the entire analytical phase of laboratory screening, i.e. not just on development and validation of chromatographical methods of analysis, but also (and especially) on preparation of biological samples prior to the actual analysis. This phase is crucial for analytes assessment, since it determines not only correct results of the laboratory examination, but also influences the lifespan of laboratory equipment, especially chromatographic columns. These days mostly traditional...
The importance of the determination of activated partial thromboplastin time and partial thromboplastin sensitivity compared to the lupus anticoagulant, deficiency of coagulation factors and heparin.
Drábková, Dagmar ; Fátorová, Ilona (advisor) ; Sadílek, Petr (referee)
The importance of the determination of activated partial thromboplastin time and partial thromboplastin sensitivity compared to the lupus anticoagulant, deficiency of coagulation factors and heparin Dagmar Drábková Abstract Activated partial thromboplastin time (aPTT) is coagulation test which detect the disturbances of hemostasis and it is the most common test for monitoring unfractioned heparin therapy. In parcial tromboplastin are present activators of contact phase and phospholipids which can significantly affect the sensitivity of reagents to coagulation factors, lupus anticoagulans and heparin. The objective of this study was firstly to compare the sensitivity of parcial tromboplastins with different origins of fosfolipids and various activators to lupus anticoagulans, to decreased and increased levels of coagulation plasmatic factor VIII and sensitivity to standard heparin. Secondly, according to measured data to determine whether any of the selected reagents is suitable for monitoring heparin while being able to detect deficit of coagulation factors and presence of inhibitor. Determination of aPTT was performed with five different reagents which had various phospholipids and activators. The measurements of aPPT were performed on automatic analyzer BCSXP. From the measured data we have found that...

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